Detection of diphtheria toxin antibodies in human sera in New Zealand by ELISA

Lau, R. C. H.

doi:10.1017/s0022172400066171

Cited by 7 publications

(3 citation statements)

References 6 publications

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“…This is particularly so when the Schick test, which is used today to distinguish the 'immune' from the 'non-immune', cannot be correlated with antibody response [17]. Although the distribution of antibody levels to DT in various populations is well documented [8, [18][19][20], there is little information on the antibody responses to individual fragments and defined epitopes. Apart from observations on a synthetic oligopeptide of the loop of fragment B (amino acids 188-201) which has been used successfully as a vaccine against lethal DT intoxication in guinea-pigs [15,16], very little is known about SPs derived from fragment A. This paper describes a survey carried out on several pools of adult human sera for antibodies to DT, and fragments A and B.…”

Section: Introductionmentioning

confidence: 99%

Human antibody response to fragments A and B of diphtheria toxin and a synthetic peptide of amino acid residues 141–157 of fragment A

Perera

Corbel

1990

Epidemiol. Infect.

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SUMMARYExamination of a selection of serum samples from adults from two regions of England showed that 50 % of men in the 16-24 years and over 55 years age groups had high titres of antibody to diphtheria toxin (DT). In contrast, only 11 % of women aged 16 to over 55 years had high titres of antibody to DT. All human antisera with high anti-DT titres reacted with a synthetic peptide (SP) corresponding to the amino acids 141-157 of DT fragment A, with sera from men aged 35 to over 55 years showing the highest titres. High antibody titres to fragment A paralleled those to SP in both sexes. Titres of antibody to DT fragment B were highest in individuals with high titres to DT. In sera from both sexes immunoglobulin G1 was the predominant subclass reactive with all three antigens. However, both IgG1 and IgG4 and to a lesser extent IgG2 and IgG3 were present in immunoglobulin concentrates.

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Section: Introductionmentioning

confidence: 99%

Human antibody response to fragments A and B of diphtheria toxin and a synthetic peptide of amino acid residues 141–157 of fragment A

Perera

Corbel

1990

Epidemiol. Infect.

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“…However, as they are time consuming, cumbersome, expensive, and require large numbers of laboratory animals, they are being replaced by different serological methods (25, 31). ELISA is such an alternative and practical method and has been widely used for measuring diphtheria antibody levels in human and animal sera due to its simplicity, ease of automation, and objective interpretation (32–34). It has been reported that ELISA correlates well with in vivo and in vitro toxin neutralization tests and immune status of humans (29, 34).…”

Section: Discussionmentioning

confidence: 99%

Effect of monophosphoryl lipid A on antibody response to diphtheria toxin and its subunits

Çağlar

Aybay

Ataoglu

2005

APMIS

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Monophosphoryl lipid A (MPL) was evaluated for its ability to enhance the antibody response to diphtheria toxin and its fragment A and fragment B subunits. BALB/c mice were immunized subcutaneously with 1 Lf of diphtheria toxoid in the presence of 25 microg of MPL on days 0 and 14. Two weeks after the second immunization, sera were obtained from the mice and analysed for antibody response to diphtheria toxin and its subunits. A new ELISA method, developed in our laboratory, was used to measure antibody levels against the toxin, fragment A, and fragment B. It was observed that MPL significantly enhanced antibody responses to diphtheria toxin and its subunits. However, there was no statistical difference between anti-A and anti-B responses. The results indicated that MPL seems to be a potential candidate as an adjuvant for future diphtheria vaccine formulation.

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“…Its introduction has played a major role in reducing the incidence of tetanus an(l diphtheria to insignificance (Lau, 1986(Lau, , 1987a even though major outbreaks of pertussis have been reported in this country (Campbell Begg, 1984;Canterbury Hospital Board, 1987).…”

Section: Introductionmentioning

confidence: 99%

Antibody level of New Zealand children immunized with the triple vaccine DTP (diphtheria-tetanus-pertussis)

Lau¹

1988

Epidemiol. Infect.

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SUMMARYEnzyme-linked immunosorbent assay (ELISA) tests were used to measure IgG antibody levels in 2638 New Zealand children who had been immunized with the triple vaccine DTP. The percentage of children immune to diphtheria decreased with age. The percentage of children immune to tetanus varied from 67-1 to 550 %. The percentage of children with measurable antibody to pertussis increased with age. The mean percentages of children with measurable antibody or immunity to one or more DTP components were 34-2 % (with 3 components), 34 4 % (2 components), and 78 1 % (1 component). It appears the immunization strategy for diphtheria and tetanus is satisfactory for herd immunity in New Zealand children. However, the current pertussis strategy may not be providing adequate immunity to 5-year-olds in this country.

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Detection of diphtheria toxin antibodies in human sera in New Zealand by ELISA

Cited by 7 publications

References 6 publications

Human antibody response to fragments A and B of diphtheria toxin and a synthetic peptide of amino acid residues 141–157 of fragment A

Human antibody response to fragments A and B of diphtheria toxin and a synthetic peptide of amino acid residues 141–157 of fragment A

Effect of monophosphoryl lipid A on antibody response to diphtheria toxin and its subunits

Antibody level of New Zealand children immunized with the triple vaccine DTP (diphtheria-tetanus-pertussis)

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