2021
DOI: 10.1094/pdis-07-20-1446-sr
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Detection of Diverse Maize Chlorotic Mottle Virus Isolates in Maize Seed

Abstract: Maize chlorotic mottle virus (MCMV) has driven the emergence of maize lethal necrosis (MLN) worldwide, where it threatens maize production in areas of East Africa, South America, and Asia. It is thought that MCMV transmission through seed may be important for introduction of the virus in new regions. Identification of infested seed lots is critical for preventing the spread of MCMV through seed. Although methods for detecting MCMV in leaf tissue are available, diagnostic methods for its detection in seed lots … Show more

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Cited by 15 publications
(20 citation statements)
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“…All seed were stored at 4˚C in sealed bags, except for those collected at R2 and R4, which were sealed and stored at -20˚C. MCMV infested and un-infested seed lots used for localization and transmission experiments were described previously [27].…”
Section: Seed Materialsmentioning
confidence: 99%
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“…All seed were stored at 4˚C in sealed bags, except for those collected at R2 and R4, which were sealed and stored at -20˚C. MCMV infested and un-infested seed lots used for localization and transmission experiments were described previously [27].…”
Section: Seed Materialsmentioning
confidence: 99%
“…Pools of poorly growing or deceased plant samples were weighed and extracted in 2 volumes (w/v) GEB. For each sample, two 100 μl aliquots were tested for the presence of MCMV by DAS-ELISA (Agdia, Elkhart, IN, USA) as previously described [27]. Within each plate, MCMV positive controls were separated from other samples by a row of buffer to prevent and monitor possible cross-contamination.…”
Section: Transmission Of MCMV Through Seedmentioning
confidence: 99%
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“…The disease may also be transmitted through infected maize seeds [ 40 ]. Diverse MCMV isolates have been detected in maize seeds [ 41 ]. Therefore, usage of virus-free seeds is important in controlling MLN in the African continent.…”
Section: Transmission Of Mln Pathogensmentioning
confidence: 99%