Detection of DNA adducts in basal and non-basal cells of the hamster trachea exposed to benzo(a)pyrene in organ culture.

Abstract

DNA adducts were quantified in hamster tracheas exposed to benzo(a)pyrene (BP) in organ culture, in basal as well as in non-basal cells, by in situ detection with an adduct-specific rabbit antiserum (W2/01) and with a mouse monoclonal antibody against human cytokeratins 5 and 8 (RCK102) to identify hamster trachea basal cells. Recognition by W2/01 of the adduct of (+)-anti-7,8-dihydmxy-9,lO-epoxide of BP (BP diolepoxide; BPDE) to deoxyguanosine (dG) was checked on human white blood ells (WBCs) exposed to BP together with 3-methylcholanthrene (3MC)-induced rat-liver miaosomes. By comparison with the adduct levels determined by 32P post-labeling, a lower detection limit of about 1 adduct per lo6 nucleotides could be deduced. Next, tracheal rings were exposed to BP (40 pM) in organ culture for 2 days, then