Detection of DNA alteration in abnormal phenotype of broiler chicken male by random amplified polymorphic DNA (RAPD)

Abstract

RAPD technique was used in this study to detect DNA band variations between both normal and abnormal male of broiler chicken based on RAPD marker. DNA polymorphisms between normal and mutant birds were detected using fifteen oligonucleiotide primers. Using these primers, DNA band loss ranged from 25 to 75%. Data demonstrated that RAPD marker could detect DNA alterations.

Molecular Investigation of Genetic Polymorphisms in Type 2 Diabetic Patients Using Random Amplified Polymorphic DNA (RAPD-PCR)

“…Random amplified polymorphic DNA (RAPD) is a PCR based technique in which random DNA fragments are amplified by single short arbitrary oligonucleotide sequences (5). It is a rapid and applicable to any organism without prior information of the nucleotide sequence presenting only qualitative results (6). RAPD analysis can detect rearrangements, additions or deletions of DNA and ploidy changes in cells by visualizing banding shifts, missing bands or the appearance of new bands in a DNA gel electrophoresis (7).…”