Detection of DNA Damage Induced by Cerium Dioxide Nanoparticles: From Models to Molecular Mechanism Activated

Souza, Tiago Alves Jorge de; Rocha, Thiago Lopes; Franchi, Leonardo Pereira

doi:10.1007/978-3-319-72041-8_13

Cited by 7 publications

(2 citation statements)

References 84 publications

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“…Although some authors have reported the genotoxic effects of CeO 2 NPs, there is no general agreement about this point [35]. It has been proposed that the apparent discordant reported data are the result of both the pro-oxidant and anti-oxidant role of CeO 2 NPs.…”

Section: Resultsmentioning

confidence: 99%

The Comet Assay as a Tool to Detect the Genotoxic Potential of Nanomaterials

et al. 2019

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The interesting physicochemical characteristics of nanomaterials (NMs) has brought about their increasing use and, consequently, their increasing presence in the environment. As emergent contaminants, there is an urgent need for new data about their potential side-effects on human health. Among their potential effects, the potential for DNA damage is of paramount relevance. Thus, in the context of the EU project NANoREG, the establishment of common robust protocols for detecting genotoxicity of NMs became an important aim. One of the developed protocols refers to the use of the comet assay, as a tool to detect the induction of DNA strand breaks. In this study, eight different NMs—TiO2NP (2), SiO2NP (2), ZnONP, CeO2NP, AgNP, and multi-walled carbon nanotubes (MWCNT)—were tested using two different human lung epithelial cell lines (A549 and BEAS-2B). The comet assay was carried out with and without the use of the formamidopyrimidine glycosylase (FPG) enzyme to detect the induction of oxidatively damaged DNA bases. As a high throughput approach, we have used GelBond films (GBF) instead of glass slides, allowing the fitting of 48 microgels on the same GBF. The results confirmed the suitability of the comet assay as a powerful tool to detect the genotoxic potential of NMs. Specifically, our results indicate that most of the selected nanomaterials showed mild to significant genotoxic effects, at least in the A549 cell line, reflecting the relevance of the cell line used to determine the genotoxic ability of a defined NM.

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Section: Resultsmentioning

confidence: 99%

The Comet Assay as a Tool to Detect the Genotoxic Potential of Nanomaterials

et al. 2019

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“…Oxidative DNA damage may cause the potential genotoxicity of the engineered Cerium nanoparticles, Nanoceria (cerium dioxide nanoparticles). Additionally, a change in ROS generation under changing pH conditions might determine the pro-or antioxidative activities of nanoceria [153]. For example, catalase and SOD mimetic activity reduce oxidative stress depending on their size at a neutral pH, whereas oxidase and peroxidase, activated at an acidic pH, may cause toxic effects and limit their usability [154,155].…”

Section: Acellular Dermal Matricesmentioning

confidence: 99%

Shielding Burn Patients from Oxidative Stress: The Therapeutic Potential of Antioxidant Membranes

Haller,

Sander,

Nischwitz

et al. 2023

Preprint

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Although a slight imbalance between oxidative and antioxidative mediators is part of normal physiology that enables cell aging and the removal of dead cells, burns disturb this equilibrium locally and systemically. Topical burn dressings may attenuate local and systemic oxidative stress and positively influence the post-burn clinical course. This review integrated knowledge regarding the impact of burn dressings on oxidative stress. Using keywords and in-text searches, literature was identified from PubMed, Google Scholar, and Google articles, and studies on local or topical applications of wound dressings and associated oxidative stress were selected. As im-balances between oxidative mediators and antioxidative agents significantly contribute to organ dysfunction and healing disturbance, we investigated oxidative stress on organs, metabolic changes, clinical results, and oxidative parameters influenced by applied dressings. We found positive local and systemic effects of external burn dressings in laboratory and animal tests; however, such studies were rare in humans. Nevertheless, we identified successful cases of semi-occlusive, occlusive, and biologically active dressings that reduce oxidative stress in hu-man burns. In particular, we highlight promising clinical and laboratory results from lac-tate-releasing dressings. Our review provides an invaluable resource for future development and clinical applications of burn dressings.

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