Detection of DNA of Plant Pathogenic Mycoplasmalike Organisms by a Polymerase Chain Reaction that Amplifies a Sequence of the 16S rRNA Gene

Ahrens, U.; Seemüller, E.

doi:10.1094/phyto-82-828

Cited by 508 publications

(290 citation statements)

References 0 publications

Supporting

Mentioning

278

Contrasting

Unclassified

Order By: Relevance

“…Phloem was removed with a knife from the bark. DNA extraction and PCR amplification were done according to Ahrens & Seemüller (1992). For the DNA amplification phytoplasma universal primer pairs (U5/U3, amplifying a fragment of about 880 bp in length) (Seemüller et al, 1994) and more specific pome-fruit phytoplasma primer pairs (fPD/rO1, amplifying a fragment of about 930 bp in length) (Lorenz et al, 1995) were used.…”

Section: Detection and Characterization Of The Pathogenmentioning

confidence: 99%

Pear decline in Spain

Avinent

Llácer

Almacellas³

et al. 1997

Plant Pathology

View full text Add to dashboard Cite

Pear decline in Spain, associated with the presence of phytoplasmas in sieve tubes, was studied. Samples of healthy and diseased pear trees were tested to confirm the presence of the pathogen. The polymerase chain reaction (PCR) technique was used, with universal and specific primers. Specimens of Cacopsylla pyri were also analysed by PCR. Phytoplasmas were detected in 79% of trees with premature reddening, in 67% of trees with weakness and necrotic spots (cv. Limonera) and in 20% of trees without symptoms. The pathogen was also detected in the psyllids, indicating that C. pyri could be the vector of the disease in Spain.

show abstract

Section: Detection and Characterization Of The Pathogenmentioning

confidence: 99%

Pear decline in Spain

Avinent

Llácer

Almacellas³

et al. 1997

Plant Pathology

View full text Add to dashboard Cite

show abstract

“…The development of universal PCR primer pairs designed to amplify all known phytoplasma 16S rDNA has facilitated this approach significantly (Ahrens & Seemu$ ller, 1992 ;Deng & Hiruki, 1991 ;Gundersen & Lee, 1996 ;Namba et al, 1993a). Moreover, nucleotide sequence analyses of the amplified 16S rDNA fragments have made phylogenetic analysis of phytoplasma isolates possible, and the information obtained has been used as the basis for delineating phytoplasmas into several groups (Kuske & Kirkpatrick, 1992 ;Schneider et al, 1995 ;Seemu$ ller et al, 1998 ;White et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

'Candidatus Phytoplasma castaneae', a novel phytoplasma taxon associated with chestnut witches' broom disease.

Jung¹,

Sawayanagi²,

Kakizawa³

et al. 2002

International Journal of Systematic and Evolutionary Microbiology

View full text Add to dashboard Cite

“…For PCR, total DNA was isolated from 1g of fresh plant material (leaf midribs, buds and stems) following the phytoplasma-enrichment procedure of Ahrens and Seemüller (1992) and stored at -20°C until processing. Samples from both affected and symptomless plants were analysed for phytoplasma infection by two PCR methods: Nested PCR and real-time PCR.…”

Section: Nested Pcr and Real-time Pcrmentioning

confidence: 99%

First identification of the pathogen causing tumor malformations in evergreen oaks in Spain

et al. 2014

View full text Add to dashboard Cite

Aim of study: In recent years an increase in pests and diseases associated with truffle plantations has been detected in Spain. The appearance of tumor malformations in trunks and branches of Quercus ilex L. must be highlighted. These bumps have expanded dramatically since the increase in the number and density of truffle plantations. This pathology is not only found in plantations, but also in forests, and in trees of all ages.Area of study: the eastern mountains and the truffle plantations of the Iberian Peninsula. Material and methods:Positive results were obtained by using two types of PCR: Real-Time PCR and nested-PCR. They were carried out with primers that amplified 16S ribosomal gene sequences that are common to all known phytoplasmas.Main result: The disease manifests itself as an irregular thickening in branches of any age and in the trunk that results in the woody tissue cracking open, forming wounds. The affected branches usually undergo necrosis and in case of affecting the trunk, the tree will eventually die. After an extensive literature review and several failed attempts to isolate fungal and bacterial species from these tumors and wounds, the disease-causing organism has been identified as a Candidatus Phytoplasma.Research highlights: The appearance of this disease may endanger the profitability of an a priori profitable crop. Due to the intrinsic characteristics of the organism, and knowing that no phytosanitary treatment is able to control phytoplasmas, future works should be directed towards identifying the transmitter in order to control the disease.

show abstract

Detection of DNA of Plant Pathogenic Mycoplasmalike Organisms by a Polymerase Chain Reaction that Amplifies a Sequence of the 16S rRNA Gene

Cited by 508 publications

References 0 publications

Pear decline in Spain

Pear decline in Spain

'Candidatus Phytoplasma castaneae', a novel phytoplasma taxon associated with chestnut witches' broom disease.

First identification of the pathogen causing tumor malformations in evergreen oaks in Spain

Contact Info

Product

Resources

About