Detection of DNA oligonucleotides with base mutations by terahertz spectroscopy and microstructures

Tang, Mingjie; Zhang, Mingkun; Yan, Shihan; Xia, Liangping; Yang, Zhongbo; Du, Chunlei; Cui, Hong‐Liang; Wei, Dongshan

doi:10.1371/journal.pone.0191515

Cited by 33 publications

(19 citation statements)

References 48 publications

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“…From Figure 3a, we can see that the absorption coefficient of the Gly23 solution increases with the increase of the frequency and there is no prominent fingerprint signal; observations which are in good agreement with the THz spectra of most biomolecular solutions [32,43,44,45]. In Figure 3b, it can be seen that the absorption coefficient of the Gly23 solution decreases with the increase of the degree of confinement at different frequencies.…”

Section: Resultssupporting

confidence: 74%

Calculated Terahertz Spectra of Glycine Oligopeptide Solutions Confined in Carbon Nanotubes

et al. 2019

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To reduce the intense terahertz (THz) wave absorption of water and increase the signal-to-noise ratio, the THz spectroscopy detection of biomolecules usually operates using the nanofluidic channel technologies in practice. The effects of confinement due to the existence of nanofluidic channels on the conformation and dynamics of biomolecules are well known. However, studies of confinement effects on the THz spectra of biomolecules are still not clear. In this work, extensive all-atom molecular dynamics simulations are performed to investigate the THz spectra of the glycine oligopeptide solutions in free and confined environments. THz spectra of the oligopeptide solutions confined in carbon nanotubes (CNTs) with different radii are calculated and compared. Results indicate that with the increase of the degree of confinement (the reverse of the radius of CNT), the THz absorption coefficient decreases monotonically. By analyzing the diffusion coefficient and dielectric relaxation dynamics, the hydrogen bond life, and the vibration density of the state of the water molecules in free solution and in CNTs, we conclude that the confinement effects on the THz spectra of biomolecule solutions are mainly to slow down the dynamics of water molecules and hence to reduce the THz absorption of the whole solution in confined environments.

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Section: Resultssupporting

confidence: 74%

Calculated Terahertz Spectra of Glycine Oligopeptide Solutions Confined in Carbon Nanotubes

et al. 2019

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“…Consequently, molecules such as amino acids (and urea as a metabolite), nucleosides, nucleotides and nucleic acids are investigated by the THz community. The characteristics of these molecules, such as temperature-dependent absorption spectra [28,48], the dielectric response of molecular solutions such as completely solvated mono-and oligonucleotides [7,51,68,119,146,155,[160][161][162], modifications of the molecular structure and spectral signatures as a function of both the polarization state of incident radiation [52] and the hydration level [94], have been reported.…”

Section: Subject Of Thz Studiesmentioning

confidence: 99%

THz Detection of Biomolecules in Aqueous Environments—Status and Perspectives for Analysis Under Physiological Conditions and Clinical use

Weisenstein

Wigger

Richter

et al. 2021

J Infrared Milli Terahz Waves

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Bioanalytical THz sensing techniques have proven to be an interesting and viable tool for the label-free detection and analysis of biomolecules. However, a major challenge for THz bioanalytics is to perform investigations in the native aqueous environments of the analytes. This review recapitulates the status and future requirements for establishing THz biosensing as a complementary toolbox in the repertoire of standard bioanalytic methods. The potential use in medical research and clinical diagnosis is discussed. Under these considerations, this article presents a comprehensive categorization of biochemically relevant analytes that have been investigated by THz sensing techniques in aqueous media. The detectable concentration levels of ions, carbohydrates, (poly-)nucleotides, active agents, proteins and different biomacromolecules from THz experiments are compared to characteristic physiological concentrations and lower detection limits of state-of-the-art bioanalytical methods. Finally, recent experimental developments and achievements are discussed, which potentially pave the way for THz analysis of biomolecules under clinically relevant conditions.

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“…THz spectroscopy was used for the qualitative and quantitative analysis of various types of nucleic acids. It was shown that when the qualitative composition of the DNA chain is changed by one nucleotide, the spectral properties of the DNA molecule is changed in the THz range that indicates the possibility of detecting DNA point mutations using THz spectroscopy [48]. This potential of THz technology may be extremely useful for intraoperative diagnosis of brain tumors.…”

Section: Determination Of Molecular Characteristics Of Tumorsmentioning

confidence: 99%

Prospects of terahertz technology in diagnosis of human brain tumors – A review

Musina

Никитин

Chernomyrdin

et al. 2020

J-BPE

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Terahertz (THz) waves feature high sensitivity to the content and state of water in biological tissues. Therefore, during the past decades, THz technology has attracted significant attention in biophotonics, including diagnosis of malignant and benign neoplasms with different nosologies and localizations. The pathophysiological features of malignant tumors of the central nervous system determine appearance of several morphological phenomena, such as increased vascularity, edema, necrosis. These phenomena cause water content increase in the studied tissues and, thus, open new ways for the THz technology applications in the intraoperative neurodiagnosis, including delineation of tumor margins. This research area is rather novel and, despite the small amount of accumulated research material, is undoubtedly extremely promising for creation of new diagnostic approaches. In this review, available results in the considered exciting branch of THz technology are summarized, and potential projections of this topic into the future are constructed.

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Detection of DNA oligonucleotides with base mutations by terahertz spectroscopy and microstructures

Cited by 33 publications

References 48 publications

Calculated Terahertz Spectra of Glycine Oligopeptide Solutions Confined in Carbon Nanotubes

Calculated Terahertz Spectra of Glycine Oligopeptide Solutions Confined in Carbon Nanotubes

THz Detection of Biomolecules in Aqueous Environments—Status and Perspectives for Analysis Under Physiological Conditions and Clinical use

Prospects of terahertz technology in diagnosis of human brain tumors – A review

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