Detection of DNA Strand Breaks in the Liver of Boleophthalmus pectinirostris Treated with Benzo(a)pyrene

Feng, Tao; Guo, Xiaoyu; Zheng, Wen-Zhu; Yuan, Dongxing

doi:10.1007/s00128-003-0159-1

Cited by 5 publications

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“…The apparent binding affinities reported here for pyr-TFOs and nonconjugated TFOs (Supporting Information Figure 1) AGRee well with our previous work on similar TFOs that bind to the supF or Aprt TFO duplex target sites (14,19,(39)(40)(41)(42). There are several published reports of the ability of pyrene-based compoundstoinduceDNAstrandbreaksand/oradducts (28)(29)(30)(31)(32)(33)(34)(35)(36)(37)(38). Geacintov et al (38) and Li et al (37) demonstrated photoinduced DNA strand cleavage in samples containing double-stranded oligonucleotides with pyrene adducts.…”

Section: Discussionsupporting

confidence: 89%

“…A promising candidate is pyrene, a polycyclic aromatic hydrocarbon (PAH). Pyrene compounds, such as benzo[ a ]pyrene (BaP) and 1-hydroxypyrene, induce DSBs as well as 8-oxo-7,8-dihydro-2′-deoxyguanosine adduct formation – . Moreover, upon UVA (355 nm) irradiation of double-stranded pyrene-substituted oligonucleotides within duplex DNA, DSBs within ∼5−7 bp of the substituted pyrene are induced at efficiencies of up to 50% , .…”

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Targeted Generation of DNA Strand Breaks Using Pyrene-Conjugated Triplex-Forming Oligonucleotides

et al. 2008

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Gene targeting by triplex-forming oligonucleotides (TFOs) 1 has proven useful for gene modulation in vivo. Photoreactive molecules have been conjugated to TFOs to direct sequence-specific damage in double-stranded DNA. However, the photoproducts are often repaired efficiently in cells. This limitation has led to the search for sequence-specific photoreactive reagents that can produce more genotoxic lesions. Here we demonstrate that photoactivated pyrene-conjugated TFOs (pyr-TFOs) induce DNA strand breaks near the pyrene moiety with remarkably high efficiency, and also produce covalent pyrene-DNA adducts. Free radical scavenging experiments demonstrated a role for singlet oxygen activated by the singlet-excited state of pyrene in the mechanism of pyr-TFO-induced DNA damage. In cultured mammalian cells, the effect of photoactivated pyr-TFO-directed DNA damage was to induce mutations, in the form of deletions, ~7-fold over background levels, at the targeted site. Thus, pyr-TFOs represent a potentially powerful new tool for directing DNA strand breaks to specific chromosomal locations for biotechnological and potential clinical applications.Triplex-forming oligonucleotides (TFOs) ‡ have been employed to modulate gene structure and function, both in vitro and in vivo. When conjugated to DNA damaging agents, TFOs can induce site-directed DNA damage resulting in enhanced mutagenesis and recombination [reviewed in (1-8)]. Reactive molecules that have been conjugated to TFOs include, but are not limited to, 2-amino-6-vinylpurine, haloacetyl amide, aryl nitrogen mustard, N 4 ,N 4 -etheno-5-methyldeoxycytidine, and various psoralen derivatives. All of these induce sitespecific cross-linking and/or alkylation in double-stranded DNA (dsDNA) (9-15). Psoralenlinked TFOs (pso-TFOs), which can induce covalent monoadducts on one strand or cross-links with both strands of duplex DNA with high efficiency and sequence specificity, are the most studied of the photoreactive TFOs. Pso-TFOs can yield site-specific DNA damage with high efficiency and have been used to introduce mutations and inactivate target genes. However, the existence of cellular mechanisms for repairing the pso-TFO photoproducts without introduction of sequence errors has limited their utility. † Funding was provided in part from National Institutes of Health (CA93729 to K.M.V.) and National Institute of Environmental Health Sciences (ES007784). 1 Abbreviations: Aprt, adenine phosphoribosyl transferase; DMF, dimethylformamide; DSB, double-strand break; dsDNA, doublestranded DNA; EMSA, electrophoretic mobility shift assay; HMWC, high molecular weight complex; PAH, polycyclic aromatic hydrocarbon; pso-, psoralen; pyr-, pyrene; strand breaks, SBs; TFO, triplex-forming oligonucleotide. NIH-PA Author ManuscriptNIH-PA Author Manuscript NIH-PA Author ManuscriptRecent efforts by several groups have improved the binding affinity and sequence specificity of TFOs in vivo. For example, Puri and colleagues found that 2'-O-aminoethyl (2'-AE) ribose substitutions within p...

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Section: Discussionsupporting

confidence: 89%

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confidence: 99%

Targeted Generation of DNA Strand Breaks Using Pyrene-Conjugated Triplex-Forming Oligonucleotides

et al. 2008

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“…Studies have showed that organic pollutants have great toxicological effects on B. pectinirostris, and could even result in the decline of the population resources (Feng et al 2001(Feng et al , 2003. B. pectinirostris mainly inhabits caves.…”

Section: Pcbs Distribution and Toxicity Risksmentioning

confidence: 99%

Distribution and Toxicity Risks of PCBs in the Tidal Flat Ecosystem Near the 37th PCBs Sealed Site on the Coast of Zhejiang Province, China

Zhang

Wang³

et al. 2014

Human and Ecological Risk Assessment: An International Journal

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This study investigated the dioxin-like (DL) PCB concentration and congener distributions in water, sediment, and Boleophthalmus pectinirostris samples collected from a beach near the 37th PCBs sealed site (the hazardous waste storage site was sealed by Zhejiang Province Environmental Monitoring Center) of Zhejiang Province, China, so as to evaluate the harmful effects of PCBs waste sealed sites on the surrounding environment. The results showed that DL-PCBs were in all samples. For all samples, the detection ratio of the mono-ortho congener PCB118 and the non-ortho congener PCB81 reached 100%, and both of their contents correlated well with the total DL-PCBs contents. Levels of low-chloride congeners were higher in environmental samples, while the enrichment of the high-chloride congeners was higher in fish. The content of PCBs in B. pectinirostris was highly related to the sediment environment, thus B. pectinirostris could well indicate environmental PCBs pollution. Considering both of the PCBs content and toxic analysis, even after 4 years of being excavated and cleaned, water, sediment, and fish samples were still at a certain degree of toxicity risks. Some areas were revealed to have a high toxicity risk, suggesting this PCBs sealed site still posed toxicity risks to the surrounding tidal flat ecosystem and might potentially endanger animals and human health.

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Effects of Aroclor 1254 on reproductive organ weight, sperm quality and testicular mitochondria oxidative stress of Boleophthalmus pectinirostris

Sun

2011

Acta Oceanol. Sin.

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Boleophthalmus pectinirostris is an amphibious economic fish and wildly distributed in the southeast coast of China. In this study, Aroclor 1254 was intraperitoneally injected into B. pectinirostris with 1, 2 and 4 µg/(g·d) for 28 d to assay the reproductive organ weight, the sperm quality (sperm concentration and motility), and the testicular mitochondrial testicular mitochondria oxidative stress. The results show that the sperm number and motility in seminal vesicles, the absolute weight of testes and seminal vesicles of B. pectinirostris treated with 2 and 4 µg/(g·d) Aroclor 1254 decreased significantly as compared to the controls (p <0.05), while those treated with 1 µg/(g·d) Aroclor 1254 had no significant effects on these indictors. For the relative weight of reproductive organs, significant reduction (p <0.05) was only observed in the seminal vesicles of B. pectinirostris treated with 4 µg/(g·d). SOD activities and GSH levels in all the Aroclor 1254 treatments were significantly lower than those of the controls (p <0.05). The activities of CAT, GPx, GR and the levels of Vit C also decreased significantly in comparison with the controls (p <0.05) at the higher dose of 2 and 4 µg/(g·d) Aroclor 1254 treatments. In addition, both H 2O2 level and MDA content in testicular mitochondria of B. pectinirostris had a close correlation with Aroclor 1254 dosage, and were significantly higher than the controls (p <0.05). Those indicate that Aroclor 1254 can induce the oxidative stress of testicular mitochondria, and impair the reproductive function of male B. pectinirostris.

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Detection of DNA Strand Breaks in the Liver of Boleophthalmus pectinirostris Treated with Benzo(a)pyrene

Cited by 5 publications

References 0 publications

Targeted Generation of DNA Strand Breaks Using Pyrene-Conjugated Triplex-Forming Oligonucleotides

Targeted Generation of DNA Strand Breaks Using Pyrene-Conjugated Triplex-Forming Oligonucleotides

Distribution and Toxicity Risks of PCBs in the Tidal Flat Ecosystem Near the 37th PCBs Sealed Site on the Coast of Zhejiang Province, China

Effects of Aroclor 1254 on reproductive organ weight, sperm quality and testicular mitochondria oxidative stress of Boleophthalmus pectinirostris

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