Detection of Donor DNA After Heart Transplantation: How Far Could It Be Affected by Blood Transfusion and Donor Chimerism?

Hubacek, Jaroslav A.; Vymětalová, Yevheniya; Bohuslavová, Romana; Kocik, M.; Málek, I

doi:10.1016/j.transproceed.2007.01.093

Cited by 7 publications

(4 citation statements)

References 5 publications

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“…Donor-derived DNA has also been detected in cell-free plasma of liver, 36 heart, 37 and kidney transplantation recipients. 38 In the case of renal transplantation, the outcome of the transplantation after an episode of acute rejection is often difficult to predict.…”

Section: Transplantationmentioning

confidence: 99%

Overview of Circulating Nucleic Acids in Plasma/Serum

Butt

Swaminathan

2008

Annals of the New York Academy of Sciences

106

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The existence of circulating nucleic acids in plasma and serum (CNAPS) was first described almost six decades ago. However, the prognostic and diagnostic utility of this circulating DNA/RNA has only really begun to be appreciated in the last decade. Earlier studies concentrated mainly on investigations concerned with fetal medicine and oncology, and significant progress was made in both specialities. More recently the field of enquiry has extended further, and attention has turned to other pathologic states, including trauma, sepsis, myocardial infarction, stroke, transplantation, diabetes mellitus, and hematologic disorders. In some of these studies, mitochondrial as well as genomic DNA and tissue-specific mRNA have been analyzed, either quantitatively or qualitatively or both, and have been shown to be modified in the presence of disease. While there is tremendous potential for CNAPS as a clinical modality, many of the emerging studies seem to be confined to a few dedicated labs. Therefore, additional independent studies are necessary in some cases in order to show reproducibility, which will further consolidate the field. Despite this shortcoming, and with the evidently increasing number of applications for CNAPS, it is highly likely that routine testing, as described, will become reality within the next 5 to 10 years.

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Section: Transplantationmentioning

confidence: 99%

Overview of Circulating Nucleic Acids in Plasma/Serum

Butt

Swaminathan

2008

Annals of the New York Academy of Sciences

106

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“…Nicméně, jak jsme popsali dříve (analýzou uměle připravených směsí produktů PCR, obsahujících různé alely v jiném poměru než 1 : 1), možnost spolehlivé detekce méně koncentrované alely končí přibližně při ředění 1 : 10 až 1 : 50. (16) Podobné výsledky byly revidovány recentně i jinými autory, (17) a zdá se tak, že použití variant VNTR pro diagnostiku Popsali jsme, že v případě analýzy sekvence specifi cké pro chromosom Y je skutečně možné detekovat přítomnost vDNA v případě akutní rejekce orgánu. (16) I tato metoda však má, jak se ukázalo, svá nečekaná úskalí daná přítomností tkáňového chimerismu.…”

Section: I S K U S Eunclassified

“…(16) Podobné výsledky byly revidovány recentně i jinými autory, (17) a zdá se tak, že použití variant VNTR pro diagnostiku Popsali jsme, že v případě analýzy sekvence specifi cké pro chromosom Y je skutečně možné detekovat přítomnost vDNA v případě akutní rejekce orgánu. (16) I tato metoda však má, jak se ukázalo, svá nečekaná úskalí daná přítomností tkáňového chimerismu. (17,18) Chimerismus je stav, kdy v jednom organismu jsou přítomny buňky jiného organismu.…”

Section: I S K U S Eunclassified

Detecting free plasma DNA in transplant medicine. Possibilities and pitfalls

Hubáček¹,

Málek²,

Vymětalová³

et al. 2008

Cor Vasa

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confidence: 99%

Early changes in cell‐free DNA levels in newly transplanted heart transplant patients

Zangwill

Kindel

Ragalie

et al. 2019

Pediatric Transplantation

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DF of cfDNA has been proposed as a stable marker for cellular injury caused by rejection in several organs including the heart. 1,2 An increased DF during episodes of rejection has been reported in both adult and pediatric heart transplant recipients. 3,4 Limited information exists regarding serial changes in DF in the immediate post-transplant phase. De Vlaminck et al 5 reports that DF declines to baseline at 1-week post-heart transplant. Similarly, in a study of DF following liver transplant, baseline levels were reached somewhere between 7 and 10 days post-transplant. 6 Just as DF has value as a specific marker for graft injury, the total amount of circulating

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Detection of Donor DNA After Heart Transplantation: How Far Could It Be Affected by Blood Transfusion and Donor Chimerism?

Cited by 7 publications

References 5 publications

Overview of Circulating Nucleic Acids in Plasma/Serum

Overview of Circulating Nucleic Acids in Plasma/Serum

Detecting free plasma DNA in transplant medicine. Possibilities and pitfalls

Early changes in cell‐free DNA levels in newly transplanted heart transplant patients

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