Detection of Dopamine and Serotonin by Competitive Enzyme-Linked Immunosorbent Assay

남궁, 수민; 최, 정수; 박, 지향; Yang, Minji; 이, 민우; 김, 성욱

doi:10.15324/kjcls.2017.49.3.220

Cited by 19 publications

(8 citation statements)

References 12 publications

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“…Namkung et al established an indirect competitive immunoassay to detect dopamine 46 . This method was effective for the detection of dopamine in urine, but was not sensitive enough at low concentrations (<0.1 μM).…”

Section: Antibody‐based Biosensorsmentioning

confidence: 99%

Biosensors and sensors for dopamine detection

Liu

2020

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196

122

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Dopamine is a key catecholamine neurotransmitter and it has critical roles in the function of the human central nervous system. Abnormal release of dopamine is related to neurological diseases and depression. Therefore, it is necessary to monitor dopamine levels in vivo and in real time to understand its physiological roles. In this review, we discuss dopamine detection focusing on the molecular recognition methods including enzymes, antibodies, and aptamers, as well as new advances based on nanomaterials and molecularly imprinted polymers (MIPs). A large fraction of these sensors rely on electrochemical detection to fulfill the requirement of fast, in situ, and in vivo detection with a high spatial and temporal resolution. These methods need to overcome interferences from molecules with a similar redox potential. In addition, fluorescent and colorimetric sensors based on aptamers are also quite popular, and care needs to be taken to validate specific dopamine binding. Combining aptamers or MIPs with electrochemistry promises to achieve rapid detection and increased selectivity. In this article, we pay more attention to the molecular recognition mechanism and critically review the sensor designs. In the end, some future directions are discussed.

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Section: Antibody‐based Biosensorsmentioning

confidence: 99%

Biosensors and sensors for dopamine detection

Liu

2020

VIEW

196

122

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“…Namkung et al proposed a competitive indirect method for DA detection, immunoassay based [ 64 ]. The ELISA transducer offered the highest sensitivity to dopamine concentration, with optical absorbance of 0.91, when anti-dopamine Ab was diluted 6000 times.…”

Section: Evolution Of the Dopamine Biosensors After Receptor Elementmentioning

confidence: 99%

From Enzymatic Dopamine Biosensors to OECT Biosensors of Dopamine

Ravariu

2023

Biosensors

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Neurotransmitters are an important category of substances used inside the nervous system, whose detection with biosensors has been seriously addressed in the last decades. Dopamine, a neurotransmitter from the catecholamine family, was recently discovered to have implications for cardiac arrest or muscle contractions. In addition to having many other neuro-psychiatric implications, dopamine can be detected in blood, urine, and sweat. This review highlights the importance of biosensors as influential tools for dopamine recognition. The first part of this article is related to an introduction to biosensors for neurotransmitters, with a focus on dopamine. The regular methods in their detection are expensive and require high expertise personnel. A major direction of evolution of these biosensors has expanded with the integration of active biological materials suitable for molecular recognition near electronic devices. Secondly, for dopamine in particular, the miniaturized biosensors offer excellent sensitivity and specificity and offer cheaper detection than conventional spectrometry, while their linear detection ranges from the last years fall exactly on the clinical intervals. Thirdly, the applications of novel nanomaterials and biomaterials to these biosensors are discussed. Older generations, metabolism-based or enzymatic biosensors, could not detect concentrations below the micro-molar range. But new generations of biosensors combine aptamer receptors and organic electrochemical transistors, OECTs, as transducers. They have pushed the detection limit to the pico-molar and even femto-molar ranges, which fully correspond to the usual ranges of clinical detection of human dopamine in body humors that cover 0.1 ÷ 10 nM. In addition, if ten years ago the use of natural dopamine receptors on cell membranes seemed impossible for biosensors, the actual technology allows co-integrate transistors and vesicles with natural receptors of dopamine, like G protein-coupled receptors. The technology is still complicated, but the uni-molecular detection selectivity is promising.

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“…Therefore, the development of techniques for the accurate and sensitive detection of dopamine is of significant interest in both clinical and research settings not only to regulate drug treatment, but also to facilitate the early diagnosis of diseases. Until now, various analytical tools have been developed and employed for dopamine assay including biochemical techniques-such as enzyme linked immuno-sorbet assay ELISA [10]; high-performance liquid chromatography-HPLC or reverse-phase HPLC [11,12]; spectrometric and spectrophotometric methods [13,14]; and capillary electrophoresis [15]. Since it was proved that dopamine electro-oxidation at conventional electrodes can be easily achieved [16]; the electrochemical approach gains a lot of popularity compared to the traditional detection methods, since electrochemical techniques are straightforward to use, generate a rapid response with minimal expense and high selectivity, and do not necessitate bulky machinery or highly trained personnel [17].…”

Section: Introductionmentioning

confidence: 99%

Enhancement of Dopamine Electrochemical Detection with Manganese Doped Crystalline Copper Oxide

et al. 2023

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Manganese doped crystalline copper oxide (CuO:Mn) and undoped CuO were prepared at room temperature by the hydrothermal method. The complete physico-chemical characterization of the materials was performed using X-ray diffraction (XRD), transmission/scanning electron microscopy (TEM/SEM), and X-ray photoelectron spectroscopy (XPS). Furthermore, their analytical applicability was tested in electrochemical experiments for a dopamine assay. According to the morphological investigation, the materials had a flat structure with nearly straight edges. The XRD analysis proved the formation of the CuO phase with good crystallinity, while the Mn doping was determined by XPS to be around 1 at.%. Under optimized conditions, at pH 5.0, the CuO:Mn modified electrode (CuO:Mn/SPE) showed a high signal for dopamine oxidation, with a linear response in the 0.1–1 µM and 1–100 µM ranges and a low limit of detection of 30.3 nM. Five times higher sensitivity for manganese doped copper oxide in comparison with the undoped sample was achieved. The applicability of the developed CuO:Mn/SPE electrode was also tested in a commercially available pharmaceutical drug with good results, suggesting that the developed sensor has promising biomedical application potential.

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Detection of Dopamine and Serotonin by Competitive Enzyme-Linked Immunosorbent Assay

Cited by 19 publications

References 12 publications

Biosensors and sensors for dopamine detection

Biosensors and sensors for dopamine detection

From Enzymatic Dopamine Biosensors to OECT Biosensors of Dopamine

Enhancement of Dopamine Electrochemical Detection with Manganese Doped Crystalline Copper Oxide

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