Detection of drug-resistance genes using single bronchoscopy biopsy specimens

Trussardi‐Régnier, Aurélie; Millot, Jean-Marc; Gorisse, Marie-Claude; C, Delvincourt; Prévost, Alain

doi:10.3892/or.18.3.703

Cited by 3 publications

(4 citation statements)

References 24 publications

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“…In addition, some studies showed that over-expression of P-gp is associated with MDR in different types of cancer, leading to poor patient prognosis [46][47][48]. We also demonstrated that increased expression of P-gp in lung tumor tissues ( Figure 4) compared with adjacent non-tumor tissues, consistent with previous reports [49][50][51]. It is worth noting that some studies have correlated P-gp overexpression with negative survival outcomes, including treatment failure and relapse [52][53][54].…”

Section: Discussionsupporting

confidence: 90%

Expression pattern of estrogen receptor β and its correlation with multidrug resistance in non-small cell lung cancer

Huang

Liu

et al. 2019

neo

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The aim of this work was to determine the expression of the ERβ (estrogen receptor β) and multidrug resistance, namely MDR1 (P-glycoprotein, P-gp), in 152 samples of non-small cell lung cancer. The expression pattern of ERβ and MDR1 were assessed by the quantitative reverse-transcriptase polymerase chain reaction (qRT-PCR) and immunohistochemistry. We also analyzed the correlation between ERβ and MDR1 with clinical and pathological data. The co-expression pattern of ERβ and individual MDR1 proteins was assessed by correspondence analysis and chi-squared tests. In the present study, we found that patients with tumor stage I-II showed higher ERβ mRNA expression levels and decreased expression of ERβ protein with increasing tumor grade, which is opposite to MDR1 expression. In addition, an opposite co-expression pattern of ERβ and individual MDR1 proteins was also observed. In conclusion, the results can be used to better understand the expression control of MDR1 and may allow for the establishment of new cancer chemistry strategies that will control P-gp expression in NSCLC.

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Section: Discussionsupporting

confidence: 90%

Expression pattern of estrogen receptor β and its correlation with multidrug resistance in non-small cell lung cancer

Huang

Liu

et al. 2019

neo

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“…However, radiation in MDR cells has also been demonstrated to mediate loss of extrachromosomally amplified MDR1 genes, concomitant with a reduction in P-gp levels (25). Fractionated irradiation led to the restoration of drug sensitivity by the downregulation of P-gp in MDR cells (26).…”

Section: Discussionmentioning

confidence: 99%

Effect of fractionated irradiation on the expression of multidrug resistance genes in the CNE1 human nasopharyngeal carcinoma cell line

Yang

Sui

et al. 2012

Molecular Medicine Reports

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Nasopharyngeal carcinoma (NPC) often develops drug resistance following radiotherapy. The molecular basis of radiotherapy-related multidrug resistance (MDR) remains unclear. In the present study, we investigated the effect of fractionated irradiation on the expression of the MDR-1 gene and the MDR-associated protein P-glycoprotein (P-gp) in CNE1 human NPC cells. CNE1 cells were treated with fractionated X-rays. Drug resistance was determined by MTT assay. The expression levels of MDR-1 and P-gp were analyzed by RT-PCR and western blot analysis, respectively. Differential expression was analyzed by gene chips. The results revealed that low levels of mRNA expression of MDR1 were present in non-irradiated CNE1 cells. Compared with the control, the expression of MDR1 mRNA was gradually increased following fractionated irradiation. On day 21, the expression of MDR1 mRNA was increased 1.59- and 2.19-fold, compared with the control, by treatment with 10 and 20 Gy, respectively. We observed decreased MDR1 expression following treatment with 10 and 20 Gy irradiation on days 28 and 35, compared with day 21. On days 21, 28 and 35, expression was increased 1.37-, 1.40- and 1.15-fold by treatment with 20 Gy compared with 10 Gy. Expression of MDR1 was significantly upregulated by treatment with 50 Gy irradiation compared with the control on days 78 and 106. P-gp expression was consistent with that of MDR1 mRNA expression. The sensitivity of CNE1 cells to cisplatin was reduced following irradiation compared with the control. A total of 26 genes were significantly upregulated and 8 genes were significantly downregulated compared with the control. Results of the present study have shown that MDR1 and P-gp are upregulated in CNE1 cells following irradiation. Multiple genes were involved in the mechanism of radiation-induced drug resistance.

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“…Biopsies from progressing cancer showed higher MDR1, MRP1 and LRP gene expression. These findings have revealed the high gene expression of MDR1 and MRP1 in relapsed diseases 53 .…”

Section: Mdr1 In Lung Tumorsmentioning

confidence: 88%

“…In the investigation by Trussardi-Regnier et al, expression of three major resistance genes MDR1, MRP1 and LRP was simultaneously investigated in small cell lung cancer, non-small cell lung cancer and metastasis 53 . Single biopsies of bronchoscopy from 73 patients were performed to investigate expression of these three resistance genes by reverse transcriptase-polymerase chain reaction.…”

Section: Mdr1 In Lung Tumorsmentioning

confidence: 99%