Detection of Echinococcus granulosus antigen by a quantum dot/porous silicon optical biosensor

Li, Yanyu; Jia, Zhenhong; Lv, Guodong; Wen, Hao; Li, Peng; Zhang, Hong Yan; Wang, Jiajia

doi:10.1364/boe.8.003458

Cited by 25 publications

(22 citation statements)

References 28 publications

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“…Here, the LOD of the immunosensors were 0.3 and 0.4 pg.mL −1 for the antibody and antigen immunosensors, respectively indicating very good sensitivity of this method. It is worth noting that the LOD of our antigen immunosensor was comparable with the reported optical sensor and faster than other immunosensors as shown in Table . However, the label‐free electrochemical sensor shown herein offers lower cost and capability of miniaturization which is essential for the point‐of‐care diagnostic applications.…”

Section: Resultsmentioning

confidence: 99%

“…To the best of our knowledge, only two optical‐based immunosensors have been reported for the detection of hydatid antigen. A quantum dot/porous silicon optical biosensor, based on fluorescence intensity, has been used for the detection of E. granulosus antigen (Egp38) . Hydatid antigen detection was also reported using the near‐infrared transmission angular spectra of porous silicon microcavity biosensor .…”

Section: Introductionmentioning

confidence: 99%

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Voltammetric Label‐free Immunosensors for the Diagnosis of Cystic Echinococcosis

2020

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Cystic echinococcosis (CE) or hydatid disease is a parasitic infection caused by Echinococcus granulosus. Early serodiagnosis and continuous monitoring of the disease is very important for medical treatment. Here, we report the detecting of both echinococcus antigen and antibody for the diagnosis of hydatid disease using square wave voltammetry (SWV)‐based immunosensors. The gold electrodes were functionalized using cysteamine/phenylene diisothiocyanate linkers and used for the immunosensors fabrication. The hydatid antigen and antibody immunosensors were constructed by the immobilization of either purified rabbit polyclonal antibody or recombinant antigen B (AgB), respectively on the functionalized gold electrodes surfaces. The detection in both cases was achieved by following the change in the SWV reduction peak current of the ferro/ferricyanide redox couple upon antibody or antigen binding. These immunosensors enabled the detection of echinococcus antigen and antibody within a concentration range of 1 pg.mL−1 to 1 μg.mL−1 with detection limits of 0.4 pg.mL−1 and 0.3 pg.mL−1, respectively. A preliminary application of the developed immunosensor was performed in spiked serum sample showing good recovery percentages ranging from 102 to 110 % for both hydatid antibody and antigen detection. This easy‐to‐use, sensitive, and low cost quantitative method holds great promise for the early diagnosis of hydatid disease and thus, better managements and treatment outcomes.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Voltammetric Label‐free Immunosensors for the Diagnosis of Cystic Echinococcosis

2020

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“…With the cavity mode to confine and enhance fluorescence, the detection limit of porous silicon microcavity biosensors has been greatly improved [85]. In addition, the fluorescent enhancement was also observed on porous silicon Bragg reflector, which in combined with quantum dots was also utilized in the detection of parasitic disease [86]. In spite of the fact that porous silicon functions as a medium for signal enhancement rather than a signal transducer in these cases, the hybrid concept might boost future bio-applications of porous silicon.…”

Section: Other Advances In Biosensingmentioning

confidence: 99%

Optical Biosensing and Bioimaging With Porous Silicon and Silicon Quantum Dots (Invited Review)

Guan¹

2017

PIER

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“…Assays based on detecting Echinococcus-derived circulating antigens were also developed and reported to have high specificity. Unfortunately, their sensitivity was relatively low [17,[19][20][21][22][23][24]. Consequently, they are barely used in clinic.…”

Section: Introductionmentioning

confidence: 99%

Targeted Sequencing of Genomic Repeat Regions Detects Circulating Cell-free Echinococcus DNA

Wan

Peng

et al. 2020

PLoS Negl Trop Dis

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Background Echinococcosis is a chronic zoonosis caused by tapeworms of the genus Echinococcus. Treatment of the disease is often expensive and complicated, sometimes requiring extensive surgery. Ultrasonographic imaging is currently the main technique for diagnosis, while immunological analysis provides additional information. Confirmation still needs pathological analysis. However, these diagnostic techniques generally detect infection in late stages of the disease. An accurate, early and non-invasive molecular diagnostic method is still unavailable. Methodology/Principal findings We sequenced the cell-free DNA (cfDNA) from plasma of echinococcosis patients and confirmed the presence of Echinococcus DNA. To improve detection sensitivity, we developed a method based on targeted next-generation sequencing of repeat regions. Simulation experiments demonstrate that the targeted sequencing is sensitive enough to detect as little as 0.1% of an Echinococcus genome in 1 mL of plasma. Results obtained using patient plasma shows that the Area Under the Curve (AUC) of the method is 0.862, with a detection sensitivity of 62.50% and specificity of 100%, corresponding to a Youden-index of 0.625. Conclusions/Significance This study provides evidence that hydatid cysts release cfDNA fragments into patient plasma. Using the repeat region targeted sequencing method, highly specific detection of Echinococcus infection was achieved. This study paves a new avenue for potential noninvasive screening and diagnosis of echinococcosis.

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Detection of Echinococcus granulosus antigen by a quantum dot/porous silicon optical biosensor

Cited by 25 publications

References 28 publications

Voltammetric Label‐free Immunosensors for the Diagnosis of Cystic Echinococcosis

Voltammetric Label‐free Immunosensors for the Diagnosis of Cystic Echinococcosis

Optical Biosensing and Bioimaging With Porous Silicon and Silicon Quantum Dots (Invited Review)

Targeted Sequencing of Genomic Repeat Regions Detects Circulating Cell-free Echinococcus DNA

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