Detection of EGFR Mutations Using Bronchial Washing-Derived Extracellular Vesicles in Patients with Non-Small-Cell Lung Carcinoma

Park, Juhee; Lee, Chaeeun; Eom, Jung Seop; Kim, Mi-Hyun; Cho, Yoon‐Kyoung

doi:10.3390/cancers12102822

Cited by 23 publications

(20 citation statements)

References 44 publications

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“…EGFR mutation was seen in exosomes of malignant pleural effusion ( Lee et al, 2018 ), and T790M mutation was also detected in exosomes of bronchoalveolar lavage fluid of patients with non-small cell lung cancer ( Park et al, 2020 ). The exosome gDNA isolated from ascites of ovarian cancer patients reflects the copy number variation of primary tumors ( Crow et al, 2017 ; Giannopoulou et al, 2019 ).…”

Section: Exosome Genomic Dna As a Tool For Colorectal Cancer Liquid B...mentioning

confidence: 99%

Roles of Exosome Genomic DNA in Colorectal Cancer

Wang

2022

Front. Pharmacol.

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Exosomes are extracellular vesicles that mediate cell-to-cell communication. Bioactive substances such as DNA, RNA, lipids, and proteins are present in it, and they play an essential role in the pathogenesis of colorectal cancer (CRC). The role of RNA and protein in exosomes has been extensively studied. Exosome DNA has recently attracted the attention of a great deal of scientists. According to studies, exosome DNA mainly contains genomic DNA (gDNA) and mitochondrial DNA (mtDNA), of which exosome gDNA is widely used in liquid biopsy of CRC. It includes a variety of clinically relevant tumor-specific mutation genes. In addition to liquid biopsy, researchers find that exosome gDNA regulates immune and metabolic functions in CRC, making it an important research object. However, the primary research on exosome gDNA is still limited. Here, we describe the occurrence and composition of exosomes. Summarize the essential characteristics and mode of action of exosome gDNA. Remarkably, this paper constitutes a comprehensive summary on the role of exosome gDNA on CRC with the intent of providing a theoretical basis and reference for early diagnosis and clinical treatment of cancer.

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Section: Exosome Genomic Dna As a Tool For Colorectal Cancer Liquid B...mentioning

confidence: 99%

Roles of Exosome Genomic DNA in Colorectal Cancer

Wang

2022

Front. Pharmacol.

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“…Furthermore, the T790M mutation was detected in 18 (72.2%) using EV-derived DNA while that was only detected in 3 patients using cell block or cytology [48]. The sensitivity of EV-derived DNA in detecting EGFR mutation was reported to be 89.7% [49]. As such, current evidence seems to suggest that liquid biopsy may be more sensitive than tissue biopsy in detecting EGFR mutations and tumour heterogeneity may pose a less significant issue in these biopsies.…”

Section: Discussionmentioning

confidence: 99%

Concordance of Cytological Specimens with Histological Tissue for Detection of Epidermal Growth Factor Receptor Mutation in Non-Small Cell Lung Cancer: A Systematic Review

Chua

Chuah

2021

Acta Cytologica

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Introduction: There is increasing need for more testing in non-small cell lung cancer given the introduction of newer targeted therapies. Cytological specimens including conventional smears (CS), cell blocks (CB), and liquid-based cytology (LBC) are an alternative to histologic tissue (HT) specimens in detecting EGFR mutations, but the concordance of these 2 specimens is yet to be determined. The aim of the present systematic review is to determine the concordance rates between different cytologic specimens with HT in detecting EGFR mutations. Methods: PubMed, Cochrane Library, and Google Scholar were utilized in the primary search, along with reference lists of electronically retrieved full-text articles. Concordance rates were pooled together if 2 or more studies reporting the same type of cytologic specimen were available. Results: Overall, 15 studies were included in this review, with 13 studies included in the pooled analysis. There was an overall concordance rate of 92.8% in 593 paired cytologic and HT specimens, with LBC having the highest concordance rate of 96.0%, followed by CS and CB, each with a concordance rate of 95.8%, although the concordance rate of CS and/or CB was lower at 90.6% with a larger pool of studies. LBC was found to have a significantly higher concordance rate than CS and/or CB. Conclusion: Cytological specimens have a high concordance rate in detecting EGFR mutations, when compared to HT. LBC has shown superior concordance rates compared to CS and CB. Cytological specimens should be considered as an additional and alternative source of diagnostic material for EGFR testing.

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“…More recently, exoDNA performed better for the diagnostic of non-small cell lung cancer (NSCLC) compared to EV-excluded cell-free DNA in bronchial wash fluid by the detection of EGFR T790M . The overall detection sensitivity of EGFR mutation was 89.7 and 31%, respectively, with 100% specificity ( Park et al, 2020 ).…”

Section: Ev-dna As a Clinically Relevant Biomarkermentioning

confidence: 99%

Next-Generation Cancer Biomarkers: Extracellular Vesicle DNA as a Circulating Surrogate of Tumor DNA

Amintas

Vendrely

Dupin

et al. 2021

Front. Cell Dev. Biol.

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Extracellular vesicles (EVs) are produced by healthy tissues and tumor cells and are released in various bodily fluids, including blood. They are limited by bilayer phospholipidic membranes, and they carry a rich content in biomolecules. Their release cleanses the cells of their waste or serves as functional local and distant cell–cell communication and molecular exchange particles. This rich and heterogeneous content has been given intense attention in cancer physiopathology because EVs support cancer control and progression. Because of their specific active cargo, they are being evaluated as carriers of liquid biopsy biomarkers. Compared to soluble circulating biomarkers, their complexity might provide rich information on tumor and metastases status. Thanks to the acquired genomic changes commonly observed in oncogenic processes, double-stranded DNA (dsDNA) in EVs might be the latest most promising biomarker of tumor presence and complexity. This review will focus on the recent knowledge on the DNA inclusion in vesicles, the technical aspects of EV-DNA detection and quantification, and the use of EV-DNA as a clinical biomarker.

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Detection of EGFR Mutations Using Bronchial Washing-Derived Extracellular Vesicles in Patients with Non-Small-Cell Lung Carcinoma

Cited by 23 publications

References 44 publications

Roles of Exosome Genomic DNA in Colorectal Cancer

Roles of Exosome Genomic DNA in Colorectal Cancer

Concordance of Cytological Specimens with Histological Tissue for Detection of Epidermal Growth Factor Receptor Mutation in Non-Small Cell Lung Cancer: A Systematic Review

Next-Generation Cancer Biomarkers: Extracellular Vesicle DNA as a Circulating Surrogate of Tumor DNA

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