Detection of ehrlichiae in African ticks by polymerase chain reaction

Parola, Philippe; Roux, Véronique; Camicas, Jean-Louis; Baradji, Issa; Brouqui, Philippe; Raoult, Didier

doi:10.1016/s0035-9203(00)90243-8

Cited by 266 publications

(155 citation statements)

References 5 publications

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“…They may represent novel bacteria. Clearly, the specificity of our broad-spectrum primers for ehrlichia-like bacteria (primers EHR16SR and EHR16SD) is not absolute, although they were designed to amplify a 345-bp fragment of the 16S rRNA gene specific for the members of the family Anaplasmataceae and have successfully been used as epidemiologic tools in Africa and Japan (8,15,17). Our first hypothesis regarding these sequences was to consider them nonspecific amplification products as a result of a PCR or sequencing error.…”

Section: Discussionmentioning

confidence: 99%

“…The DNA extracted from the ticks was also screened as described previously with primers EHR16SR and EHR16SD (Bioprobe Systems), which amplify a 345-bp fragment of the 16S rRNA gene of bacteria within the family Anaplasmataceae, including the genera Anaplasma, Ehrlichia, Neorickettsia, and Wolbachia (17). To amplify the main part of the 16S rRNA gene, tick DNA samples that were found to be positive with the primers described above were subjected to a second PCR with primers EHR16SR and EHR16SD coupled with universal primers fD1 and rp2, as described previously (8).…”

Section: Tickmentioning

confidence: 99%

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Detection of Ehrlichia spp., Anaplasma spp., Rickettsia spp., and Other Eubacteria in Ticks from the Thai-Myanmar Border and Vietnam

et al. 2003

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A total of 650 ticks, including 13 species from five genera, were collected from animals, from people, or by flagging of the vegetation at sites on the Thai-Myanmar border and in Vietnam. They were tested by PCR to detect DNA of bacteria of the order Rickettsiales. Three Anaplasma spp. were detected in ticks collected in Thailand, including (i) Anaplasma sp. strain AnDa465, which was considered a genotype of Anaplasma platys (formerly Ehrlichia platys) and which was obtained from Dermacentor auratus ticks collected from dogs; (ii) Anaplasma sp. strain AnAj360, which was obtained from Amblyomma javanense ticks collected on a pangolin; and (iii) Anaplasma sp. strain AnHl446, which was closely related to Anaplasma bovis and which was detected in Haemaphysalis lagrangei ticks collected from a bear. Three Ehrlichia spp. were identified, including (i) Ehrlichia sp. strain EBm52, which was obtained from Boophilus microplus ticks collected from cattle from Thailand; (ii) Ehrlichia sp. strain EHh324, which was closely related to Ehrlichia chaffeensis and which was detected in Haemaphysalis hystricis ticks collected from wild pigs in Vietnam; and (iii) Ehrlichia sp. strain EHh317, which was closely related to Ehrlichia sp. strain EBm52 and which was also detected in H. hystricis ticks collected from wild pigs in Vietnam. Two Rickettsia spp. were detected in Thailand, including (i) Rickettsia sp. strain RDla420, which was detected in Dermacentor auratus ticks collected from a bear, and (ii) Rickettsia sp. strain RDla440, which was identified from two pools of Dermacentor larvae collected from a wild pig nest. Finally, two bacteria named Eubacterium sp. strain Hw124 and Eubacterium sp. strain Hw191 were identified in Haemaphysalis wellingtoni ticks collected from chicken in Thailand; these strains could belong to a new group of bacteria.

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Section: Discussionmentioning

confidence: 99%

Section: Tickmentioning

confidence: 99%

Detection of Ehrlichia spp., Anaplasma spp., Rickettsia spp., and Other Eubacteria in Ticks from the Thai-Myanmar Border and Vietnam

et al. 2003

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“…Para la familia Anaplasmataceae se utilizaron oligonucleótidos para un fragmento de 16S ARNr 21 , utilizándose como control positivo A. bovis. A las muestras positivas se les realizó dos RPC específicas para A. platys: fragmento de 16S ARNr y del gen groESL, según los procedimientos indicados por los autores 22,23 .…”

Section: Amplificaciónunclassified

Detección molecular de Rickettsia massiliae y Anaplasma platys en garrapatas Rhipicephalus sanguineus y caninos domésticos del municipio de Bahía Blanca (Argentina)

Cicuttin¹,

Vidal

Salvo³

et al. 2014

Rev. chil. infectol.

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“…All of the noninfected animals had normal values for all three hematological parameters (i.e., RBC count, Hb, and HCT); hence, other agents that could have contributed to the anemia among the animals could be ruled out. In addition, all the DNA samples were PCR screened for the presence of Anaplasma species as described previously (9,12). Because only a single animal in the noninfected category was found to be infected with Anaplasma bovis, possible involvement of anaplasmosis as a cause of the anemia could be excluded.…”

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confidence: 99%

PCR Detection of Babesia ovata from Cattle Reared in Japan and Clinical Significance of Coinfection with Theileria orientalis

et al. 2012

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We describe here the clinical significance of coinfection with Theileria orientalis and Babesia ovata in cattle. Anemia status in a herd of dairy cattle in Japan was investigated in relation to infection with these parasites. Our findings indicate that while B. ovata infection might not be the primary cause of anemia in the cattle, it may contribute to the clinical development of anemia in animals coinfected with both B. ovata and T. orientalis .

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Detection of ehrlichiae in African ticks by polymerase chain reaction

Cited by 266 publications

References 5 publications

Detection of Ehrlichia spp., Anaplasma spp., Rickettsia spp., and Other Eubacteria in Ticks from the Thai-Myanmar Border and Vietnam

Detection of Ehrlichia spp., Anaplasma spp., Rickettsia spp., and Other Eubacteria in Ticks from the Thai-Myanmar Border and Vietnam

Detección molecular de Rickettsia massiliae y Anaplasma platys en garrapatas Rhipicephalus sanguineus y caninos domésticos del municipio de Bahía Blanca (Argentina)

PCR Detection of Babesia ovata from Cattle Reared in Japan and Clinical Significance of Coinfection with Theileria orientalis

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