Detection of encephalomyocarditis virus infection in animal cells by gas liquid chromatography

Levanon, Avigdor; Klibansky, Y.; Kohn, Alexander

doi:10.1007/bf01935132

Cited by 2 publications

(2 citation statements)

References 11 publications

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“…in the cell membrane, leading to leakage of some intracellular components into the surrounding medium (Levanon, Klibansky, and Kohn, 1973;Sinyak el al., 1970). Since the relevant metabolic changes occur on a very small scale [in the nano or picogram level (Mitruka and Alexander, 1967)] their detection by conventional chemical methods may be very difficult.…”

Section: Introductionmentioning

confidence: 99%

Picorna‐ and togavirus infection of cells detected by gas chromatography

Levanon

Klibansky

Kohn

1977

Journal of Medical Virology

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Viral infection of cells causes chemical and metabolic changes, which can be detected by gas chromatography (GC) of ether extracts of supernatant fluids and cell homogenates before any significant damage to the cells is observable microscopically. The characteristic and specific GC patterns obtained from BHK-21 and Vero cell cultures infected with encephalomyocarditis, polio, echoviruses, and a togavirus make it possible to distinguish between these infecting viruses. The appearance of 1 or 2 compounds, represented by GC peaks with TR values of 302 and 677 seconds seems to be specific for these viruses. Other peaks found in the supernatant media 1-2 hours after infection probably represent cell constituents leaking into the medium as a result of damage to the cell membrane by the invading virus.

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Section: Introductionmentioning

confidence: 99%

Picorna‐ and togavirus infection of cells detected by gas chromatography

Levanon

Klibansky

Kohn

1977

Journal of Medical Virology

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“…The feasibility of classifying microorganisms by the chemical analytical method of gas chromatography was originally suggested by Abel et al (1). Subsequently, their approach has been applied to a wide variety of microorganisms, including blue-green algae (15), marine and estuarine bacteria (20), viruses (16), mycobacteria (23), clostridia (19), micrococci (11), bacilli (12), pseudomonads (3), and streptococci (2, 8,21).…”

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confidence: 99%

Fatty acid fingerprints of Streptococcus mutans NCTC 10832 grown at various temperatures

Drucker¹,

Veazey²

1977

Appl Environ Microbiol

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Fatty acid fingerprints were determined gas chromatographically for Streptococcus mutans NCTC 10832, which had been grown in batch brain heart infusion at a series of nine temperatures ranging from 29.0 to 40.0°C. The major acids at all temperatures were n-palmitic and octadecenoic acids. Other acids detected at all temperatures included n-myristic, palmitoleic, n-stearic, and eicosenoic acids. An increase in temperature resulted in a decrease in the proportion of unsaturated to saturated fatty acids, indicating the importance of accurate temperature control in such gas-liquid chromatographic-chemotaxonomic studies.

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Detection of encephalomyocarditis virus infection in animal cells by gas liquid chromatography

Cited by 2 publications

References 11 publications

Picorna‐ and togavirus infection of cells detected by gas chromatography

Picorna‐ and togavirus infection of cells detected by gas chromatography

Fatty acid fingerprints of Streptococcus mutans NCTC 10832 grown at various temperatures

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