Detection of endocrine disrupting chemicals in Danio rerio and Daphnia pulex: Step-one, behavioral screen

Alla, Lakshmi Neha Reddy; Monshi, Manahil; Siddiqua, Zoha; Shields, Jeremiah N.; Alame, Karim; Wahls, Andrea; Akemann, Camille; Meyer, Danielle; Crofts, Emily J.; Saad, Fadie; El-Nachef, Judy; Antoon, Merna; Nakhle, Raquel; Hijazi, Nemer; Hamid, Maha; Gurdziel, Katherine; McElmurry, Shawn P.; Kashian, Donna R.; Baker, Tracie R.; Pitts, David K.

doi:10.1016/j.chemosphere.2020.129442

Cited by 11 publications

(8 citation statements)

References 94 publications

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“…Metformin is used for the treatment of polycystic ovary syndrome (PCOS) with its benefits attributed to improving insulin sensitivity, reducing insulin levels and thereby reducing the activity of steroidogenic enzymes. Conversely, in males the use of metformin has been linked to genital defects in male offspring [36] that may be linked to reports of effects of metformin on human and mouse testicular cells, lowers testosterone levels, and also reports in fish that it is an endocrine disruptor [464][465][466][467][468][469] The effects of metformin on the microbiota in the gut where the release of GLP-1 from L-cells that in turn enhances the release of insulin from ß-cells in the pancreas. The anti-cancer effects of metformin have been attributed to the activation of AMPK and subsequent inhibition of mTOR.…”

Section: Discussionmentioning

confidence: 99%

“…There are no known metabolites of metformin, and it may accumulate and negatively affect numerous organisms as has already reported in species of fish. At levels found in waste water, it is reported to act as an endocrine disrupter [467,468] in Danio rerio (zebrafish) and Daphnia pulex (water fleas) with behavioural effects seen in zebrafish at concentrations as low as 10 nM [469]. A conservative estimate based on 150 million people taking 1 g/day of metformin is that potentially 150,000 kg/day of the drug are voided in the urine reflecting an increasing concern on the health of numerous species including Homo sapiens.…”

Section: Discussionmentioning

confidence: 99%

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Metformin: Is it a drug for all reasons and diseases?

et al. 2022

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Metformin: Is it a drug for all reasons and diseases?

et al. 2022

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“…Metformin may interact with a variety of molecular targets across species [ 29 ]. In relation to its actions on aquatic species, there is literature on its impact on fecundity and behaviour in fish [ 30 , 31 ] and therefore, metformin has been attributed as an endocrine disruptor [ 32 ]. There are few biochemical data available for daphnids; however, the mechanism of action of metformin has been linked to the induction of the hypoxia-inducible factor (HIF) α and β genes [ 33 ].…”

Section: Discussionmentioning

confidence: 99%

The Effects of Single and Combined Stressors on Daphnids—Enzyme Markers of Physiology and Metabolomics Validate the Impact of Pollution

Michalaki

McGivern²,

Poschet³

et al. 2022

Toxics

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The continuous global increase in population and consumption of resources due to human activities has had a significant impact on the environment. Therefore, assessment of environmental exposure to toxic chemicals as well as their impact on biological systems is of significant importance. Freshwater systems are currently under threat and monitored; however, current methods for pollution assessment can neither provide mechanistic insight nor predict adverse effects from complex pollution. Using daphnids as a bioindicator, we assessed the impact in acute exposures of eight individual chemicals and specifically two metals, four pharmaceuticals, a pesticide and a stimulant, and their composite mixture combining phenotypic, biochemical and metabolic markers of physiology. Toxicity levels were in the same order of magnitude and significantly enhanced in the composite mixture. Results from individual chemicals showed distinct biochemical responses for key enzyme activities such as phosphatases, lipase, peptidase, β-galactosidase and glutathione-S-transferase. Following this, a more realistic mixture scenario was assessed with the aforementioned enzyme markers and a metabolomic approach. A clear dose-dependent effect for the composite mixture was validated with enzyme markers of physiology, and the metabolomic analysis verified the effects observed, thus providing a sensitive metrics in metabolite perturbations. Our study highlights that sensitive enzyme markers can be used in advance on the design of metabolic and holistic assays to guide the selection of chemicals and the trajectory of the study, while providing mechanistic insight. In the future this could prove to become a useful tool for understanding and predicting freshwater pollution.

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“…Aldrin/dieldrin is the contaminant most associated with oxidative damage in the livers of two wild fish species at the Furnas Hydroelectric Power Station (HPS) reservoir (Minas Gerais, Brazil) ( Sakuragui et al, 2013 ). After 24 h exposure, dieldrin significantly affected Danio rerio and Daphnia pulex ’s swimming behavioral response and sensitivity ( Alla et al, 2021 ). As a teratogen, dieldrin can alter the expression of dopamine receptor 2A and dopamine transporter in zebrafish ( Danio rerio ) embryos ( Sarty et al, 2017 ).…”

Section: Toxicity Of Aldrin and Dieldrinmentioning

confidence: 99%

Microbial Degradation of Aldrin and Dieldrin: Mechanisms and Biochemical Pathways

Pang

Lin

et al. 2022

Front. Microbiol.

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As members of the organochlorine group of insecticides, aldrin and dieldrin are effective at protecting agriculture from insect pests. However, because of excessive use and a long half-life, they have contributed to the major pollution of the water/soil environments. Aldrin and dieldrin have been reported to be highly toxic to humans and other non-target organisms, and so their use has gradually been banned worldwide. Various methods have been tried to remove them from the environment, including xenon lamps, combustion, ion conversion, and microbial degradation. Microbial degradation is considered the most promising treatment method because of its advantages of economy, environmental protection, and convenience. To date, a few aldrin/dieldrin-degrading microorganisms have been isolated and identified, including Pseudomonas fluorescens, Trichoderma viride, Pleurotus ostreatus, Mucor racemosus, Burkholderia sp., Cupriavidus sp., Pseudonocardia sp., and a community of anaerobic microorganisms. Many aldrin/dieldrin resistance genes have been identified from insects and microorganisms, such as Rdl, bph, HCo-LGC-38, S2-RDLA302S, CSRDL1A, CSRDL2S, HaRdl-1, and HaRdl-2. Aldrin degradation includes three pathways: the oxidation pathway, the reduction pathway, and the hydroxylation pathway, with dieldrin as a major metabolite. Degradation of dieldrin includes four pathways: oxidation, reduction, hydroxylation, and hydrolysis, with 9-hydroxydieldrin and dihydroxydieldrin as major products. Many studies have investigated the toxicity and degradation of aldrin/dieldrin. However, few reviews have focused on the microbial degradation and biochemical mechanisms of aldrin/dieldrin. In this review paper, the microbial degradation and degradation mechanisms of aldrin/dieldrin are summarized in order to provide a theoretical and practical basis for the bioremediation of aldrin/dieldrin-polluted environment.

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Detection of endocrine disrupting chemicals in Danio rerio and Daphnia pulex: Step-one, behavioral screen

Cited by 11 publications

References 94 publications

Metformin: Is it a drug for all reasons and diseases?

Metformin: Is it a drug for all reasons and diseases?

The Effects of Single and Combined Stressors on Daphnids—Enzyme Markers of Physiology and Metabolomics Validate the Impact of Pollution

Microbial Degradation of Aldrin and Dieldrin: Mechanisms and Biochemical Pathways

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