Detection of enteroviruses in treated drinking water

Vivier, J.C.; Ehlers, M.M.; Grabow, W. O. K.

doi:10.1016/s0043-1354(01)00433-x

Cited by 79 publications

(47 citation statements)

References 22 publications

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“…En este sentido, es necesario intensificar los esfuerzos para detectar y En este análisis se demostró la persistencia de los virus entéricos después del tratamiento del agua para consumo humano en algunos municipios de Colombia. En varios países se ha reportado la presencia de virus en aguas tratadas (14,15,(30)(31)(32). Los datos de la vigilancia de la calidad del agua en Colombia evidencian que existe un alto porcentaje de fuentes de agua que no cumplen con el patrón de potabilidad según los indicadores bacteriológicos.…”

Section: Discussionunclassified

Presencia de virus entéricos en muestras de agua para el consumo humano en Colombia: desafíos de los sistemas de abastecimiento

et al. 2016

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Introducción. El agua de consumo humano puede ser vehículo de transmisión de agentes patógenos. La detección de virus entéricos en estas muestras de agua es esencial para establecer las acciones adecuadas de control y prevención de las enfermedades asociadas. Objetivo. Analizar los resultados del diagnóstico de virus entéricos en muestras de agua para el consumo humano recibidas en el Instituto Nacional de Salud y establecer su asociación con los datos sobre la calidad del agua en los municipios de Colombia. Materiales y métodos. Se hizo un análisis descriptivo retrospectivo de los resultados obtenidos en la detección de rotavirus, enterovirus, virus de la hepatitis A y adenovirus, en muestras de agua recibidas para estudios complementarios en la investigación de brotes de hepatitis entérica, de enfermedad diarreica aguda y de enfermedades transmitidas por alimentos. Dicha información se correlacionó con los datos de la vigilancia de la calidad del agua municipal determinada según el índice de riesgo de la calidad del agua (IRCA). Resultados. Se procesaron 288 muestras de 102 municipios de Colombia, de las cuales el 50,7 % fue positivo para algún virus: 26,73 %, para el virus de la hepatitis A; 20,48 %, para enterovirus y rotavirus, y 18,05 % para adenovirus. Se detectaron virus en 48,26 % de las muestras de agua no tratada y en 45,83 % de las de agua tratada. El IRCA no mostró correlación con la presencia de virus. Conclusiones. La presencia de virus en el agua representa un riesgo para la salud pública. La prevención de la transmisión de virus por medio del agua requiere políticas para fortalecer los sistemas de suministro y para mejorar la vigilancia epidemiológica.

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Section: Discussionunclassified

Presencia de virus entéricos en muestras de agua para el consumo humano en Colombia: desafíos de los sistemas de abastecimiento

et al. 2016

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“…After a 14-day incubation period, which included a blind passage at day 7 post-infection, the infected cells were harvested for molecular analysis for human adenoviruses [21] and enteroviruses [20], as well as for blind passage onto monolayers of Vero African Green Monkey cell line (ECACC 84113001) in cell culture tubes with flying coverslips. After a further incubation of 7 days at 37 xC, the infected cell cultures were examined for cytopathic effects, stained with haematoxylin and eosin and examined for virusspecific inclusion bodies [22].…”

Section: Virus Isolationmentioning

confidence: 99%

Diverse norovirus genotypes identified in sewage-polluted river water in South Africa

et al. 2012

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SUMMARYThis study aimed to assess norovirus (NoV) contamination and genotype diversity in surface water in Gauteng, South Africa. Between January 2008 and December 2010, three rivers, namely Klip, Suikerbosrant, and Rietspruit were monitored for NoV genogroup (G)I and GII. Viruses were recovered using the glass wool adsorption-elution technique and detected by real-time reverse transcription-polymerase chain reaction. From 2008 to 2010, NoVs were detected in 66% (70/106) of Klip river samples. The Rietspruit and Suikerbosrant rivers were contaminated with NoV in 95 % (20/21) and 21 % (5/24) of samples, respectively. NoV-positive samples comprised of 33 % GI, 29% GII and 38 % of both GI and GII strains. Based on partial capsid gene analysis (region C), 16 NoV genotypes (6 GI, 10 GII) were identified. The major genotypes detected were GI.4, GI.5 and GII.4. These rivers could be a potential source of NoV infection for communities using the water for domestic or recreational purposes.

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“…Viral recovery and concentration techniques include ultrafiltration (Soule et al 2000;Divizia et al 1989a;Garin et al 1994), adsorption-elution using filters or membranes (Gilgen et al 1997;Passagot et al 1985;Senouci et al 1996), glass wool (Vilaginès et al 1993;Vilaginès et al 1997) or glass powder (Gajardo et al 1991;Menut et al 1993), two-phase separation with polymers (Schwab et al 1993), flocculation (Nasser et al 1995;Backer 2002), and the use of monolithic chromatographic columns (Branovic et al 2003;Kramberger et al 2004;Kovac et al 2009;Gutierrez-Aguirre et al 2009). The use of the glass wool adsorption-elution procedure for the recovery of enteric viruses from large volumes of water has proven to be a cost-effective method and has successfully been applied for the routine recovery of human enteric viruses from large volumes of water in the South African setting (Taylor et al 2001;Van Heerden et al 2004, 2005Vivier et al 2001Vivier et al , 2002Vivier et al , 2004, 2006Venter 2004). This method can be adapted for the in-line recovery of viruses from water (Grabow et al 1996) which circumvents transporting of large volumes of potentially highly polluted water great distances to a central laboratory which would be expensive and a potential health hazard.…”

Section: Sampling For Viruses In Watermentioning

confidence: 99%

Analytical Methods for Virus Detection in Water and Food

et al. 2010

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Potential ways to address the issues that relate to the techniques for analyzing food and environmental samples for the presence of enteric viruses are discussed. It is not the authors' remit to produce or recommend standard or reference methods but to address specific issues in the analytical procedures. Foods of primary importance are bivalve molluscs, particularly, oysters, clams, and mussels; salad crops such as lettuce, green onions and other greens; and soft fruits such as raspberries and strawberries. All types of water, not only drinking water but also recreational water (fresh, marine, and swimming pool), river water (irrigation water), raw and treated sewage are potential vehicles for virus transmission. Well over 100 different enteric viruses could be food or water contaminants; however, with few exceptions, most well-characterized foodborne or waterborne viral outbreaks are restricted to hepatitis A virus (HAV) and calicivirus, essentially norovirus (NoV). Target viruses for analytical methods include, in addition to NoV and HAV, hepatitis E virus (HEV), enteroviruses (e.g., poliovirus), adenovirus, rotavirus, astrovirus, and any other relevant virus likely to be transmitted by food or water. A survey of the currently available methods for detection of viruses in food and environmental matrices was conducted, gathering information on protocols for extraction of viruses from various matrices and on the various specific detection techniques for each virus type.

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Detection of enteroviruses in treated drinking water

Cited by 79 publications

References 22 publications

Presencia de virus entéricos en muestras de agua para el consumo humano en Colombia: desafíos de los sistemas de abastecimiento

Presencia de virus entéricos en muestras de agua para el consumo humano en Colombia: desafíos de los sistemas de abastecimiento

Diverse norovirus genotypes identified in sewage-polluted river water in South Africa

Analytical Methods for Virus Detection in Water and Food

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