Detection of Environmental Contaminants by RAPD Method

Pal, Subhasis

doi:10.20546/ijcmas.2016.508.060

Cited by 10 publications

(3 citation statements)

References 12 publications

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“…However, some of this damage may not be revealed by the methods used widely at present. the enormous advances and developments in molecular biology tools during the last three decades offer various new possibilities for detecting DNa damage through DNa markers (Castellani et al, 1993;savva et al, 1994;savva, 1996, 1998enan, 2006;al-Qurainy 2010 andPal, 2016). Consequently, in this work, we investigated the possibility of using DNa fingerprinting by iPBS technique that is based on ltR retrotransposons and serve as a general marker system to detect DNa damage in cowpea genome for alteration in response to treatments with plant oils and the bio-insecticide abamectin.…”

Section: Resultsmentioning

confidence: 99%

iPBS-DNA fingerprinting used to detect genotoxic effects of three natural plant oils and the bio-insecticide abamectin.

Mahfouz

Barakat

2018

Egypt. J. Bot.

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E sseNtial oils and the bio-insecticides have been utilized to protect the plants aginst different kind of insects. However, it is really improtent to study the side effects of these essential oils and insecticides on plants. the iPBs technique based on long terminal Repeat (ltR) retrotransposons was applied in this study to detect the DNa damage in cowpea seeds exposed to three plant oils of Trigonella foenum-graecum, Panax ginseng, Origanum majorana and the bio-insecticide abamectin. Different levels of polymorphism ranged from 37.50% polymorphism for primer 2394 to 82.35% polymorphism for primer 2270 with a total average of 52.00%. also, decrease in genetic template stability (Gts%) values were recorded with all treatments as compared with the control. the value of Band sharing index (Bsi) for each treatment was calculated and the results showed that mixture 2 (lC20 abamectin + 2lC95 ginseng) showed the lowest (Bsi) as compared with all treatments. the obtained results, suggested that the assurance should be strongly taken in the consideration for the safety, efficacy, and toxicity of the preparation derived from plants. Furthermore, iPBs assay is a powerful tool for the detection of the genotoxic effects of environmental chemicals and offer great promise for future especially for the determination of genetic damage following exposure to contaminants.

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Section: Resultsmentioning

confidence: 99%

iPBS-DNA fingerprinting used to detect genotoxic effects of three natural plant oils and the bio-insecticide abamectin.

Mahfouz

Barakat

2018

Egypt. J. Bot.

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“…These changes in the RAPD reaction patterns of the bands may be attributable to DNA damage caused by genotoxins, such as structural alterations or rearrangements (Atienzar et al, 2002). The RAPD method is very useful for ecotoxicological studies because it is simple, sensitive, cheap, and effective at detecting DNA damage (De Wolf et al, 2004;Pal 2016).…”

Section: Introductionmentioning

confidence: 99%

Water pollution evaluation in Aswan, Egypt, utilizing biochemical and molecular indicators in aquatic Chironomidae larvae in natural and laboratory settings

al.¹

2022

Egypt. J. of Aquatic Biolo. and Fish.

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Heavy metal pollution has a harmful impact on the health of all living beings. The goal of this study was to compare the impacts of zinc (Zn), lead (Pb), iron (Fe), and manganese (Mn) on two Egyptian streams in Aswan Governorate. In addition, aquatic Chironomidae larvae were exposed to a variety of heavy metal concentrations for two days. The mortality rate was determined, and the median lethal concentrations (LC50) were estimated. The most harmful metal for Chironomidae larvae was Pb, followed by Zn, Fe, and Mn. Metal bioconcentration in Chironomidae larvae increases with increasing concentrations of metals. The enzymatic response to heavy metals uptake in Chironomidae larvae; namely, lipid peroxidation, superoxide dismutase, catalase, glutathione-S-transferase, and glutathione peroxidase were determined. Although the Pb-treated group recorded the highest lipid peroxidation, it showed the lowest glutathione peroxidase activity. The glutathione-S-transferase enzyme was less active in the Zntreated group, as was the superoxide dismutase enzyme, and the catalase enzyme was the least active in the Zn-treated group. Changes in randomly amplified polymorphic DNA (RAPD) profiles were detected following heavy metal treatments at various doses when compared to controls. This study gives insight into how cruise ship activities and industrial pollution in rivers endanger aquatic life and hence, human life.

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“…On the other hand, the RAPD fingerprinting technique, a PCR-based molecular marker technique, is a fundamental tool, being a simple, rapid, and low-cost assay used to detect genotoxins that induce a wide range of DNA damage to plants (point mutations, inversions, deletions). It can also be used to describe similarities and diversification between samples by the appearance of new unique bands, disappearance of polymorphic bands, and variation in band intensities (Abdelhaliem and AL-Huqail, 2016;Pal, 2016) Taking the above into consideration, the present study aimed: 1) to evaluate possible stimulatory or inhibitory influences of nTiO 2 as well as phytotoxicity of heavy metal lead (Pb) on soybean seeds; 2) to test the efficiency of the stimulatory (optimal) dose of nTiO 2 as an adsorbent material to neutralize and alleviate the phytotoxic action of lead on economic crop plant soybean, and; 3) to apply nTiO 2 in agriculture for protection of economic crops and to increase agricultural production in future.…”

Section: Introductionmentioning

confidence: 99%

Research Article Attenuation of lead genotoxicity in <i>Glycine</i> <i>max</i> by adsorbent nanosized titanium dioxide using phenotypic, cytogenetic and DNA status bioassays

Abdelhaliem¹,

Al-Shalawi²

2019

Genet. Mol. Res.

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©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 18 (3): gmr18350 E. Abdelhaliem et al. 2doses of Pb induced notable inhibitory and genotoxic impacts on all biomarkers used, in a dose-dependent manner. We conclude that the powdered state of optimal dose (10 mg) had a good ability to adsorb Pb onto its surface and consequently mitigated its toxicity. This was evident through the significant amelioration of parameters of each biomarker after application of the three Pb adsorbate solutions on soybean seeds. Therefore, we suggest that stimulatory and adsorbent nTiO 2 dose may be used in the future to protect against heavy metal toxicity in economically important plants.

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Detection of Environmental Contaminants by RAPD Method

Cited by 10 publications

References 12 publications

iPBS-DNA fingerprinting used to detect genotoxic effects of three natural plant oils and the bio-insecticide abamectin.

iPBS-DNA fingerprinting used to detect genotoxic effects of three natural plant oils and the bio-insecticide abamectin.

Water pollution evaluation in Aswan, Egypt, utilizing biochemical and molecular indicators in aquatic Chironomidae larvae in natural and laboratory settings

Research Article Attenuation of lead genotoxicity in <i>Glycine</i> <i>max</i> by adsorbent nanosized titanium dioxide using phenotypic, cytogenetic and DNA status bioassays

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