Detection of Enzyme Activity through Catalytic Signal Amplification with Functionalized Gold Nanoparticles

Bonomi, Renato; Cazzolaro, Alessandro; Sansone, Anna; Scrimin, Paolo; Prins, Leonard J.

doi:10.1002/anie.201007389

Cited by 93 publications

(63 citation statements)

References 33 publications

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“…Prins et al were also able to utilise intrasteric regulation of a synthetic catalyst for a colourimetric enzyme detection assay (Scheme 29). 172 In this example, gold nanoparticles, covered with triazacyclononane (TACN) ligands capable of binding Zn 2+ , were prepared and the resulting cationic complexes were shown to effectively catalyse transphosphorylation reactions. 173 However, this catalytic activity could be switched off through the binding of oligoanions, such as peptides, to the catalyst through electrostatic interactions, which physically block the active site of the catalyst.…”

Section: Single-catalyst Signal Amplificationmentioning

confidence: 99%

Approaches towards molecular amplification for sensing

Goggins

Frost

2016

Analyst

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Diagnostic assays that rely on molecular interactions have come a long way; from initial reversible detection systems towards irreversible reaction indicator-based methods. More recently, the emergence of innovative molecular amplification methodologies has revolutionised sensing, allowing diagnostic assays to achieve ultra-low limits of detection. There have been a significant number of molecular amplification approaches developed over recent years to accommodate the wide variety of analytes that require sensitive detection. To celebrate this achievement, this comprehensive critical review has been compiled to give a broad overview of the many different approaches used to attain amplification in sensing with an aim to inspire the next generation of diagnostic assays looking to achieve the ultimate detection limit. This review has been created with the focus on how each conceptually unique molecular amplification methodology achieves amplification, not just its sensitivity, while highlighting any key processes. Excluded are any references that were not found to contain an obvious molecular amplifier or amplification component, or that did not use an appropriate signal readout that could be incorporated into a sensing application. Additionally, methodologies where amplification is achieved through advances in instrumentation are also excluded. Depending upon the type of approach employed, amplification strategies are divided into four categories: target, label, signal or receptor amplification. More recent, more complex protocols combine a number of approaches and are therefore categorised by which amplification component described within was considered as the biggest advancement. The advantages and disadvantages of each methodology are discussed along with any limits of detection, if stated in the original article. Any subsequent use of the methodology within sensing or any other application is also mentioned to draw attention to its practicality. The importance of amplification within sensing is wholly emphasised while perspectives on the future direction of the field are also shared.

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Section: Single-catalyst Signal Amplificationmentioning

confidence: 99%

Approaches towards molecular amplification for sensing

Goggins

Frost

2016

Analyst

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“…The chemical or electrostatic attachment of enzymes to the nanoparticles has resulted in enhancement, retention or inhibition of catalytic activity of the enzyme16171819202122 that inspired the design of enzyme biosensors. On the other hand, the spatially confined environment of enzymes could be anticipated to facilitate the crystallization of inorganic materials with nanometer precisions232425.…”

mentioning

confidence: 99%

Biogenic Growth of Alloys and Core-Shell Nanostructures Using Urease as a Nanoreactor at Ambient Conditions

Sharma

Mandani

Sarma

2013

Sci Rep

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Biomineralization is an extremely efficient biologically guided process towards the advancement of nano-bio integrated materials. As a prime module of the natural world, enzymes are expected to play a major role in biogenic growth of inorganic nanostructures. Although there have been developments in designing enzyme-responsive nanoparticle systems or generation of inorganic nanostructures in an enzyme-stimulated environment, reports regarding action of enzymes as reducing agents themselves for the growth of inorganic nanoparticles still remains elusive. Here we present a mechanistic investigation towards the synthesis of metal and metallic alloy nanoparticles using a commonly investigated enzyme, Jack bean urease (JBU), as a reducing as well as stabilizing agent under physiological conditions. The catalytic functionality of urease was taken advantage of towards the development of metal-ZnO core-shell nanocomposites, making urease an ideal bionanoreactor for synthesizing higher order nanostructures such as alloys and core- shell under ambient conditions.

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“…16). 56 The enzyme assay relies on the use of a peptidic enzyme substrate that also functions as an inhibitor of TACNÁZn 2+ catalytic activity, which are functionalized onto gold nanoparticles. Upon hydrolysis by the enzyme, the peptidic substrate/inhibitor is fragmented, losing affinity for the monolayer surface.…”

Section: Protein Sensingmentioning

confidence: 99%

Monolayer protected gold nanoparticles with metal-ion binding sites: functional systems for chemosensing applications

et al. 2015

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In this review we describe the use of monolayer protected gold nanoparticles (Au NPs) for chemosensing applications. The attention is focused on a special subclass of Au NPs, namely those that contain binding sites for metal ions in the monolayer. It will be shown that these systems are very well-equipped for metal ion sensing as the complexation of the metal ions can affect the properties of the system in many ways leading to detectable output signals even at very low analyte concentrations. In addition, the presence of metal ions in the monolayer themselves can serve as recognition units for the highly selective interaction with small organic molecules or biomacromolecules. Key examples will be discussed that underscore the attractive properties and potential of this class of Au NPs as components of chemosensing assays.

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Detection of Enzyme Activity through Catalytic Signal Amplification with Functionalized Gold Nanoparticles

Cited by 93 publications

References 33 publications

Approaches towards molecular amplification for sensing

Approaches towards molecular amplification for sensing

Biogenic Growth of Alloys and Core-Shell Nanostructures Using Urease as a Nanoreactor at Ambient Conditions

Monolayer protected gold nanoparticles with metal-ion binding sites: functional systems for chemosensing applications

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