Detection of Escherichia coli from Imported and Local Beef Meat in Mosul City

Al-Chalaby, Aamer Y.

doi:10.22207/jpam.14.1.39

Cited by 2 publications

(1 citation statement)

References 4 publications

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“…The E. coli strain is typically resident in the digestive system of animals and humans (Bekal et al, 2003). They are regarded as commensal organisms with low virulence but can still cause opportunistic infections outside the intestine locations (Quinn et al, 2011;Al-Chalaby, 2020). The extra-intestinal infection related to E. coli strains was associated with acquired genetic factors that assist E. coli in resisting immune body immune defense and colonization, invasion and spread to all body parts (Kabir, 2010).…”

Section: Introductionmentioning

confidence: 99%

Multiplex PCR for ompT and iss genes of Escherichia coli isolated from chronic respiratory disease (CRD) broiler farms

Raheed, B. Y.,

Alchalaby, A. Y. H.,

Al-Aalim, A. M.

et al. 2024

MJM

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Aims:The aim of this was to isolate Escherichia coli from broilers infected with chronic respiratory disease (CRD) and investigate the prevalence of outer membrane protein T (ompT) and increased serum survival (iss) genes. Methodology and results: Fifty trachea and lung samples were cultured for isolation of E. coli and molecular confirmation was done for these isolates using conventional PCR. Multiplex PCR technique was used to detect the existence of ompT and iss genes of E. coli. Results showed that the percentage of E. coli isolation reached 42% from all trachea and lung samples by using conventional procedures. However, the polymerase chain reaction (PCR) diagnosis method recorded 28% when using the uidA gene (genus-specific gene) for E. coli. Neither virulence genes (ompT and iss) were detected in all E. coli isolated in this study. Conclusion, significance and impact of study: Escherichia coli is the principal causative agent of CRD infections, which can bear different combinations of virulence genes that play significant roles in the pathogenicity of bacteria. All isolates in the existing study underwent APEC strains that didn't contain virulence genes like ompT and iss.

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Section: Introductionmentioning

confidence: 99%

Multiplex PCR for ompT and iss genes of Escherichia coli isolated from chronic respiratory disease (CRD) broiler farms

Raheed, B. Y.,

Alchalaby, A. Y. H.,

Al-Aalim, A. M.

et al. 2024

MJM

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Microbiological Evaluation of Local and Imported Raw Beef Meat at Retail Sites in Oman with Emphasis on Spoilage and Pathogenic Psychrotrophic Bacteria

Al-Mazrouei,

Al-Kharousi,

Al-Kharousi

et al. 2024

Microorganisms

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Determining the microbial quality and safety of meat is crucial because of its high potential to harbor pathogens. To address the critical knowledge gap and shed light on potential contamination risk in the meat supply chain, this study aimed to assess the underexplored microbial quality and safety of marketed beef meat in Oman. Thirty-three beef meat samples from six hypermarkets were analyzed for Aerobic Plate Count (APC), Psychrotrophic Bacteria Count (PBC), and coliform and Escherichia coli counts. Prevalences were 93% and 94% (means: 2.8 ± 1.1 and 2.6 ± 0.8 log CFU/g, respectively) for coliform, and 80% and 83% (means: 1.8 ± 1.4 and 1.7 ± 0.9 log CFU/g, respectively) for E. coli in imported and local samples, respectively. The mean counts of APC (6.3 ± 0.1 log CFU/g) and PBC (6.2 ± 0.2 log CFU/g) were statistically similar but different from those of coliform and E. coli. Bacterial identification using VITEK 2 compact revealed spoilage bacteria (Pseudomonas luteola, Pseudomonas fluorescens, and Shewanella putrefaciens) and pathogenic bacteria (Acinetobacter bumannii complex, Aerococcus viridans, Enterococcus faecalis, and Oligella ureolytica), which demonstrates a potential for both spoilage and pathogen-related risks. It is concluded that the APC counts of all samples exceeded acceptable standards set by the G.C.C. Standardization Organization (GSO), which was established to protect food safety and public health in Oman and other Gulf countries. This suggests an increased risk of spoilage and pathogen contamination. This study provides one of the earliest reports of microbial contamination levels in meat, serving as an eye-opener for policymakers and stakeholders. It highlights a need for stricter hygiene protocols and improved meat handling and processing practices to enhance meat safety and protect public health in Oman and the Gulf region.

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Detection of Escherichia coli from Imported and Local Beef Meat in Mosul City

Cited by 2 publications

References 4 publications

Multiplex PCR for ompT and iss genes of Escherichia coli isolated from chronic respiratory disease (CRD) broiler farms

Multiplex PCR for ompT and iss genes of Escherichia coli isolated from chronic respiratory disease (CRD) broiler farms

Microbiological Evaluation of Local and Imported Raw Beef Meat at Retail Sites in Oman with Emphasis on Spoilage and Pathogenic Psychrotrophic Bacteria

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