Detection of Escherichia coli in drugs with antibody conjugated quantum dots as immunofluorescence probes

Wu, Sheng-Mei; Jing, Chen; Ai, Xiao-Xia; Yan, Zhengyu

doi:10.1016/j.jpba.2013.01.005

Cited by 17 publications

(9 citation statements)

References 15 publications

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“…The IgY was covalently bonded to QDs by coupling agents N -(3-dimethylaminopropyl)- N ’- ethylcar-bodiimide hydrochloride (EDC, Sigma) and N -hydroxysuccinimide (NHS, Sigma) in MEST [23]. The 10 μL QDs were mixed with 200 μL NHS (10 mg/mL) and 200 μL EDC (10 mg/mL).…”

Section: Methodsmentioning

confidence: 99%

A Rapid Detection Method of Brucella with Quantum Dots and Magnetic Beads Conjugated with Different Polyclonal Antibodies

Song

Liu

et al. 2017

Nanoscale Res Lett

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Brucella spp. are facultative intracellular bacteria that cause zoonotic disease of brucellosis worldwide. Traditional methods for detection of Brucella spp. take 48–72 h that does not meet the need of rapid detection. Herein, a new rapid detection method of Brucella was developed based on polyclonal antibody-conjugating quantum dots and antibody-modified magnetic beads. First, polyclonal antibodies IgG and IgY were prepared and then the antibody conjugated with quantum dots (QDs) and immunomagnetic beads (IMB), respectively, which were activated by N-(3-dimethylaminopropyl)-N’-ethylcar-bodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) to form probes. We used the IMB probe to separate the Brucella and labeled by the QD probe, and then detected the fluorescence intensity with a fluorescence spectrometer. The detection method takes 105 min with a limit of detection of 103 CFU/mL and ranges from 10 to 105 CFU/mL (R 2 = 0.9983), and it can be well used in real samples.

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Section: Methodsmentioning

confidence: 99%

A Rapid Detection Method of Brucella with Quantum Dots and Magnetic Beads Conjugated with Different Polyclonal Antibodies

Song

Liu

et al. 2017

Nanoscale Res Lett

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“…However, PCR techniques often have high risk of false results owing to inhibition by components of the sample matrix and complicated pretreatment process such as extraction of the whole or a part of the pathogen DNA [5]. ELISA technique also requires extra-labeled antibodies that are costly and time-consuming steps [7].…”

Section: Introductionmentioning

confidence: 99%

Development of Single-Walled Carbon Nanotube-Based Biosensor for the Detection of Staphylococcus aureus

Choi

Lee

Park

et al. 2017

Journal of Food Quality

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The goal of this research is to develop a single-walled carbon nanotube-(SWCNT-) based biosensor to detect Staphylococcus aureus. The specificity of 11 bacteria and polyclonal anti-Staphylococcus aureus antibodies (pAbs) was determined using an indirect ELISA. The pAbs were immobilized onto sensor platform after the hybridization of 1-pyrenebutanoic acid succinimidyl ester (PBASE). The resistance difference (Δ ) was calculated using a potentiostat. The bacteria detected by the biosensor were observed using a scanning electron microscope (SEM). The optimum concentration of SWCNTs on the platform was determined to be 0.1 mg/mL. The binding of pAbs with S. aureus resulted in a significant increase in resistance value of the biosensor ( < 0.05). The SEM images confirmed the specific binding of S. aureus on the biosensor. The SWCNT-based biosensor was able to detect S. aureus with a limit of detection (LOD) of 4 log CFU/mL.

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“…The overall process flow of the linkers binding, antibodies immobilization on suspended MoS 2 FET through macro storage fluidic channel to detect E. coli as shown in the schematic of Fig. 4 (a) ( Gunda et al, 2014 ; Wu et al, 2013 ). The I D -V LG transfer curve of the linkers, antibodies, PBS buffer solution, and the 100 CFU/mL of E. coli bacteria was performed, as demonstrated in Fig.…”

Section: Resultsmentioning

confidence: 99%

Reliable and highly sensitive biosensor from suspended MoS2 atomic layer on nano-gap electrodes

Masurkar

Thangavel

Yurgelevic

et al. 2021

Biosensors and Bioelectronics

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The uneven morphology and the trapped charges at the surface of the traditionally used supporting substrate-based 2D biosensors produces a scattering phenomenon, which leads to irregular signal from individually fabricated device. Though suspended 2D channel material has the potential to overcome scattering effects from the substrates but achieving reliability and selectivity, have been limiting the biosensor technology. Here, we have demonstrated nanogap electrodes fabrication by using the self-assembly technique, which provides suspension to the 2D-MoS 2 . These nano-spacing electrodes not only give suspension but also provide robust strength to the atomic layer, which remains freestanding after coating of the Hafnium oxide (HfO 2 ) as well as linkers and antibodies. For evaluating the electrical characteristics of suspended MoS 2 FET, gating potential was applied through electrolyte on suspended MoS 2 transistor, which achieved lower subthreshold swing 70 mV/dec and ON/OFF ratio 10 7 . Afterward, pH detection has demonstrated at room temperature, which shows an impressive sensitivity of ∼880 by changing 1 unit of pH. Besides, we have successfully shown Escherichia coli ( E. coli ) bacteria sensing from suspended MoS 2 transistor by functionalizing dielectric layer with E. coli antibodies. The reported biosensor has shown the ∼9% of conductance changes with a lower concentration of E. coli (10 CFU/mL; colony-forming unit per mL) as well as maintain the constant sensitivity in three fabricated devices. This type of the architecture has a potential to detect range of biomolecules such as COVID-19 viruses also by altering the oxide surface.

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Detection of Escherichia coli in drugs with antibody conjugated quantum dots as immunofluorescence probes

Cited by 17 publications

References 15 publications

A Rapid Detection Method of Brucella with Quantum Dots and Magnetic Beads Conjugated with Different Polyclonal Antibodies

A Rapid Detection Method of Brucella with Quantum Dots and Magnetic Beads Conjugated with Different Polyclonal Antibodies

Development of Single-Walled Carbon Nanotube-Based Biosensor for the Detection of Staphylococcus aureus

Reliable and highly sensitive biosensor from suspended MoS2 atomic layer on nano-gap electrodes

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