Detection of Escherichia coli O157:H7 using chicken immunoglobulin Y

An immunoenzymatic assay for the diagnosis of hepatitis A utilising immunoglobulin Y

“…Additionally, the amount of IgY produced in egg yolk is threefold greater than the amount of IgG in the same volume of rabbit serum (Cova 2005), the production expense is less than IgG (Cova 2005), IgY presents a reduction in background colour of immunoenzymatic assays when compared to IgG (Schade et al 2005) and the extraction of IgY is simpler than IgG (can be made merely by egg yolk collection instead of animals bleeding) (Cova 2005). Because of these advantages, IgY has been successfully used in immunoenzymatic assays for the detection of Schistosoma japonicum (Lei et al 2009), foot-and-mouth disease virus (Veerasami et al 2008), sendai virus in porcines (Kvietkauskaite & Acaite 2004) and Escherichia coli (Sunwoo et al 2006).…”

Rapid Immunochromatographic Assay for <i>Escherichia coli</i> O157:H7 in Bovine Milk Using IgY Labeled by Fe₃O₄/Au Composite Nanoparticles

“…As can be seen from use IgY as a detection antibody showed the detection limit of 10 3 CFU mL _ 1 by ELISA (Sunwoo et al 2006) . The ICA developed using IgY as the detection antibodies here can achieve a detection limit of 10 3 CFU mL _ 1 without pretreatment of the milk samples, which can be also analyzed by MS intensity with a detection limit of 10 2 CFU mL _ 1 .…”

“…O157:H7 IgY was produced as previously reported (Sunwoo et al 2006). Purified IgY was used as a detection antibodies and conjugated to GoldMag (GM, Shaanxi Lifegen Co., Ltd, China).…”

Detection of circulating antigens in serum samples of mice experimentally infected with Trichinella spiralis by a sandwich ELISA based on IgY

“…Furthermore, chicken IgY recognize more epitopes than the corresponding mammalian antibodies (Svendsen & Hau, 2006). Because of the above advantages, IgY has been widely used to diagnosis of different diseases (Sunwoo et al, 2006;Dias da Silva & Tambourgi, 2010).…”