Detection of Escherichia coli O157:H7 and Shiga toxin 2a gene in pork, pig feces, and clean water at Jagalan slaughterhouse in Surakarta, Central Java Province, Indonesia

Goma, Maria Kristiani Epi; Indraswari, Alvita; Haryanto, Aris; Widiasih, Dyah Ayu

doi:10.14202/vetworld.2019.1584-1590

Cited by 14 publications

(13 citation statements)

References 22 publications

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“…On the other hand, most pig isolates carried stx 2a only. An Indonesian study reported that STEC O157:H7 from pigs ( n = 7) all had stx 2a 74 ; however, only stx 2a was examined in that study. There are a total of 14 genomes of STEC O157:H7 pig isolates deposited in GenBank (2 from China and 12 from USA), with six, six, and two of the isolates having both stx 1a and stx 2a , stx 2c and stx 1a , respectively (Table S8 ).…”

Section: Discussionmentioning

confidence: 95%

Genomic analysis of Shiga toxin-producing Escherichia coli O157:H7 from cattle and pork-production related environments

et al. 2021

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Three E. coli O157:H7 outbreaks have been attributed to contaminated pork in Alberta, Canada, recently. This study investigates the phylogenetic relatedness of E. coli O157:H7 from pigs, cattle, and pork-production environments for source attribution. Limited strain diversity was observed using five conventional subtyping methods, with most or all strains being in one subgroup. Whole-genome single nucleotide polymorphism analysis confirmed the recent ancestry of the isolates from all three sources. Most environmental isolates clustered closer with pig isolates than cattle isolates. Also, a direct link was observed between 2018-outbreak environmental isolates and isolates collected from a pig farm in 2018. The majority of pig isolates harbor only one Shiga toxin gene, stx2a, while 70% (35/50) of the cattle isolates have both stx1a and stx2a. The results show some E. coli O157:H7 strains could establish persistence on pig farms and as such, pigs can be a significant source of the organism.

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Section: Discussionmentioning

confidence: 95%

Genomic analysis of Shiga toxin-producing Escherichia coli O157:H7 from cattle and pork-production related environments

et al. 2021

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“…Thus, the new method of z3276-LAMP-LF in our study effectively detects lower concentrations of E. coli O157:H7. Third, the performance of many current methods for the detection of E. coli O157:H7 has been evaluated in artificially contaminated samples (Ravan et al 2016;Zhang et al 2013;Li and Chen 2012) as well as practical samples collected from slaughterhouses and farms (Holland et al 2000;Fei et al 2020;Goma et al 2019). In this study, we evaluated the performance of the z3276-LAMP-LF assay using artificially contaminated ground beef and bovine feces.…”

Section: Discussionmentioning

confidence: 99%

Rapid Detection of Escherichia coli O157:H7 by Loop-Mediated Isothermal Amplification Coupled with a Lateral Flow Assay Targeting the z3276 Genetic Marker

et al. 2021

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The most commonly reported serotype of Shiga toxin-producing Escherichia coli (STEC) is O157:H7. This pathogen presents a threat to public health and is a cause of foodborne illness worldwide. The efficient and sensitive detection of E. coli O157:H7 remains a challenge for food safety. In this report, we developed a sensitive and specific loop-mediated isothermal amplification (LAMP) reaction coupled with a lateral flow (LF) assay to rapidly detect E. coli O157:H7. Six specific primers were targeted to the genetic marker z3276. Two loop primers were labeled with either fluorescein isothiocyanate (FITC) or digoxin (Dig), which facilitated analysis by the LF assay. The LAMP-LF assay detected E. coli O157:H7, and no crossreactivity was observed with the other bacterial strains tested, including non-O157 STEC and other E. coli. The detection limit of the LAMP-LF assay was 1.3 CFU/mL for E. coli O157:H7 in broth culture, which was of equal or higher sensitivity than the other amplicon detection methods tested (agarose electrophoresis and dye). Analysis of samples from slaughterhouses indicted that the z3276-LAMP-LF assay was superior in terms of sensitivity, accuracy, and rapidity in detecting E. coli O157:H7 compared with conventional PCR. A further advantage of this method is that it is not dependent upon specialized equipment or techniques and therefore has wide potential applications in the field.

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“…e infection caused by these bacteria initially causes nonbleeding diarrhea accompanied by abdominal cramps. en, it may develop into bloody diarrhea and hemolytic uremic syndrome (HUS), which causes kidney failure in humans [10][11][12].…”

Section: Introductionmentioning

confidence: 99%

Escherichia coli O157 : H7 from Food of Animal Origin in Arsi: Occurrence at Catering Establishments and Antimicrobial Susceptibility Profile

Geresu

Regassa

2021

The Scientific World Journal

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Escherichia coli O157 : H7 (E. coli O157 : H7) has been found to be the major cause of food-borne diseases and a serious public health problem in the world, with an increasing concern for the emergence and spread of antimicrobial-resistant strains. Hitherto, little is known about the carriage of E. coli O157 : H7 and its antimicrobial susceptibility profile in the food of animal origin in Ethiopia. This study aimed to determine the occurrence and multidrug resistance profile of E. coli O157 : H7 from food of animal origin at different catering establishments in the selected study settings of Arsi Zone. One hundred ninety-two animal origin food items, namely, raw/minced meat (locally known as “Kitfo,” “Kurt,” and “Dulet”), raw milk, egg sandwich, and cream cake samples were collected and processed for microbiological detection of E. coli O157 : H7. Out of 192 samples, 2.1% (4/192) were positive for E. coli O157 : H7. Two E. coli O157 : H7 isolates were obtained from “Dulet” (6.3%) followed by “Kurt” (3.1%, 1/32) and raw milk (3.1%, 1/32), whereas no isolate was obtained from “Kitfo,” egg sandwich, and cream cake samples. Of the 4 E. coli O157 : H7 isolates subjected to 10 panels of antimicrobial discs, 3 (75%) were highly resistant to kanamycin, streptomycin, and nitrofurantoin. Besides, all the isolates displayed multidrug resistance phenotypes, 3 to 5 antimicrobial resistance, amid kanamycin, streptomycin, nitrofurantoin, tetracycline, and chloramphenicol. The occurrence of multidrug-resistant E. coli O157 : H7 isolates from foods of animal origin sampled from different catering establishments reveals that the general sanitary condition of the catering establishments, utensils used, and personnel hygienic practices did not comply with the recommended standards. Thus, this finding calls for urgent attention toward appropriate controls and good hygienic practices in different catering establishments dealing with consuming raw/undercooked foods of animal origin.

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Detection of Escherichia coli O157:H7 and Shiga toxin 2a gene in pork, pig feces, and clean water at Jagalan slaughterhouse in Surakarta, Central Java Province, Indonesia

Cited by 14 publications

References 22 publications

Genomic analysis of Shiga toxin-producing Escherichia coli O157:H7 from cattle and pork-production related environments

Genomic analysis of Shiga toxin-producing Escherichia coli O157:H7 from cattle and pork-production related environments

Rapid Detection of Escherichia coli O157:H7 by Loop-Mediated Isothermal Amplification Coupled with a Lateral Flow Assay Targeting the z3276 Genetic Marker

Escherichia coli O157 : H7 from Food of Animal Origin in Arsi: Occurrence at Catering Establishments and Antimicrobial Susceptibility Profile

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