Detection of Escherichia coli Pathotypes from the Cases of Diarrhea

Zarringhalam, Maryam; Goudarzi, Hossein; Nahaei, Mohammad Reza; Bandehpour, Mojgan; Shahbazi, Gholamhassan

doi:10.13005/bbra/2028

Cited by 5 publications

(3 citation statements)

References 24 publications

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“…In Iran, different studies found different incidence ranges from 1.1% to 7.4% in humans with diarrhea. Rates of incidence varied in different studies due to different age of patients and the size of taken samples (22)(23)(24)(25)(26). Alizade et al in 2014 found 36 STEC isolates out of 117 samples from immunocompromised (HIV or Thalassemia) cases (30.7%) (27) which is far more than other studies, comparing with 34.6% of incidence in pet birds included in our study.…”

Section: Discussionmentioning

confidence: 47%

Diverse Antimicrobial Resistance Profiles Across Phylogroups of Shiga Toxin-Producing Escherichia coli Isolates in Companion Birds

Piryae,

Abbasi,

Peighambari

et al. 2023

J Poult Sci Avian Dis

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Escherichia coli (E. coli) is a very important indicator bacteria that could be used for tracking the evolution of antibiotic resistance in both humans and animals. Shiga toxin-producing E. coli (STEC) strains are responsible for the worldwide development of human intestinal disease and potentially fatal hemorrhagic colitis or hemolytic uremic syndrome which could be transferred to humans from companion animals including pet birds. A total of 200 fecal samples were collected from birds with different situations of health status, age, and sex which have been referred to the University of Tehran’s Veterinary Hospital. 26 isolates of E. coli (13%) were found from different species of birds. 9 Attaching-effacing (AEEC) isolates (34.6%) were found in this study and all of them (9 out of 9 isolates; 100%) were STEC isolates. Phylogroup analysis revealed that 4 isolates belonged to B2 and 3 isolates belonged to D phylogroup, also 2 of the isolates were un-typable. The results of the disk diffusion method showed that 7 out of 9 STEC isolates (77.7%) were considered to be multi-drug resistance (MDR). 9 out of 9 (100%) isolates were resistant to penicillin and erythromycin, and sensitive to fosfomycin and lincospectin. In B2 phylogroup, almost all the isolates were sensitive to fluoroquinolones (96.9% sensitive). In the D phylogroup, the results were different, and the isolates were 87.5% resistant or they were developing resistance against fluoroquinolones. The results of this study revealed that different species of birds which are being kept as common pets in Iran, can be affected with STEC strains and also, they could be identified as multi-drug resistant E. coli, which doubles the importance of these findings, especially for public health.

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Section: Discussionmentioning

confidence: 47%

Diverse Antimicrobial Resistance Profiles Across Phylogroups of Shiga Toxin-Producing Escherichia coli Isolates in Companion Birds

Piryae,

Abbasi,

Peighambari

et al. 2023

J Poult Sci Avian Dis

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“…The prevalence of STEC strains in children or adults fecal samples has been investigated in several studies. A review article found these results: 5 out of 395(1.3%) from children and adults with diarrhea (Taghadosi, 2018), 36 out of 117(30.7%) from humans with HIV or thalassemia (Alizade, 2017), 34 out of 685(4.9%) children with diarrhea (Mohammadi-Sardo, 2017), 11 out of 147(7.4%) from stool samples of E.coli-positive strains collected from a human with diarrhea (Zarringhalam, 2016),15 out of 285(5.3%) in children <2 years old with diarrhea (Abbasi, 2014), 7 out of 615(1.1%) from children <5 years old with diarrhea (Kargar& Homayoon 2009). The evidence reveals a significant frequency of STEC in gastroenteritis patients (Hooman et al, 2020a).…”

Section: Discussionmentioning

confidence: 94%

Phylogenetic Analysis of Attaching and Effacing E. coli Strains Isolated From Pet Birds in Iran

Abbasi,

Peighambari,

Razmyar

2024

IJVM

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Background: Enteropathogenic E. coli (EPEC) and Shiga toxin-producing E. coli (STEC) are categorized as attaching and effacing E. coli (AEEC) due to their eae gene. One of the essential causes of diarrhea in humans is AEEC, which affects birds, too, thereby being considered a zoonotic pathogen. Objectives: Our study aimed to determine AEEC and evaluate its antibiotic resistance and phylogroups. Methods: A total of 200 fecal samples were collected from pet birds referred to the Veterinary Medicine Hospital, University of Tehran. PCR methods were used to detect AEEC using uspA, eae, bfpA, stx1, and stx2 gene-specific primers. The antimicrobial susceptibility of the recovered isolates was determined by the agar disk diffusion and MIC methods. Their phylogroups were analyzed based on Clermont phylotyping methods. Results: Of 200 samples, we isolated 26 (13%) E. coli strains, 9 harbor eae genes. None of the ease-positive samples possessed the bfpA gene, but 4 had stx2, and 5 had stx1 and stx2 genes. Phylogenetic analysis identified the phylogenetic groups of all AEEC isolated strains but 2 (duck and cockatiel). Detected phylogroups include four B2 and three D. Based on our results, 7 out of 9 AEEC isolated strains showed multi-drug resistance. Conclusion: The discovery of common phylogroups of AEEC in pet birds (a common companion animal in Iran with intimate contact with their owners, especially children) and humans, as well as their resistance to a wide range of antibiotics used in human medicine, verifies AEEC as a serious public health threat.

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“…Pathogenic Escherichia coli are one of the major causative agents of food poisoning accidents occurring in Korea and abroad. Pathogenic Escherichia coli infect human through contaminated food and drinking water [1][2][3] Pathogenic Escherichia coli can be divided into five types according to the pathological mechanism, and some Escherichia coli have high pathogenicity [2]. In 2011, numerous food poisoning accidents caused by Escherichia coli O104 were reported in Europe, most of which were fatal [3,4].…”

Section: Introductionmentioning

confidence: 99%

Status and Prospects of PCR Detection Methods for Diagnosing Pathogenic Escherichia coli : A Review

Yim¹,

Kun-Ho²,

Chon³

et al. 2021

J. Dairy Sci. Biotechnol.

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Escherichia coli are the predominant facultative bacteria found in the gastrointestinal tract of animals and humans. Some strains of E. coli that acquire virulence factors and cause foodborne and waterborne diseases in humans are called pathogenic E. coli and can be divided into five pathotypes according to the virulence mechanism: EAEC, EHEC, EIEC, EPEC, and ETEC. Although selective media have been developed to detect E. coli, distinguishing pathogenic strains from non-pathogenic ones is difficult because of their similar biochemical properties. Therefore, it is very important to find a new and effective diagnostic method to identify pathogenic E. coli. With recent advances in molecular biology and whole genome sequencing, the use of polymerase chain reaction (PCR) is increasing rapidly. In this review paper, we provide an overview of pathogenic E. coli and present a review on PCR detection methods that can be used to diagnose pathogenic E. coli. In addition, the possibility of real-time PCR incorporating IAC is introduced. Consequently, this review paper will contribute to solving the current challenges related to the detection of pathogenic E. coli.

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Detection of Escherichia coli Pathotypes from the Cases of Diarrhea

Cited by 5 publications

References 24 publications

Diverse Antimicrobial Resistance Profiles Across Phylogroups of Shiga Toxin-Producing Escherichia coli Isolates in Companion Birds

Diverse Antimicrobial Resistance Profiles Across Phylogroups of Shiga Toxin-Producing Escherichia coli Isolates in Companion Birds

Phylogenetic Analysis of Attaching and Effacing E. coli Strains Isolated From Pet Birds in Iran

Status and Prospects of PCR Detection Methods for Diagnosing Pathogenic Escherichia coli : A Review

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