Detection of ethyl glucuronide in dried human blood using LC-MS/MS

Abstract

Ethyl glucuronide, as a direct metabolite of ethanol degradation, has proven useful as a long-term marker in many forensic applications. The inability to determine ethyl glucuronide in dried blood left a missing link in many investigations. Here, we describe a new method based on mass spectrometry in a Pauli-type ion trap in order to determine this substance in dried blood samples.

Assessment of the stability of the ethanol metabolite ethyl sulfate in standardised degradation tests

“…It is formed by sulfotransferase enzymes when ethanol is consumed. Both substances are used as a proof for alcohol consumption if found in urine, blood, other body fluids or hair of a person suspected of drinking [1][2][3][4][5][6][7][8][9]. There is evidence, that EtG can be produced and degraded by bacteria present in the sample matrix, putting into question the reliability of EtG as an alcohol consumption marker [10][11][12][13].…”

Ethyl glucuronide determination in meconium and hair by hydrophilic interaction liquid chromatography–tandem mass spectrometry

“…Extensive alcohol use can lead to severe mental, physical and social issues. For this reason, scientific research aiming to detect markers of alcohol use in different human matrices has gained interest in recent years [1][2][3][4][5].…”

A study of ethyl glucuronide in post-mortem blood as a marker of ante-mortem ingestion of alcohol

“…After our work, another kinetic study of EtG and EtS in the serum of social drinkers was carried out (134) and an LC/MS method for EtG in plasma (135) and an LC-MS/MS method for serum (136) as well as for dried blood (137) have been published. Also, EtG and EtS were measured simultaneously in serum with LC-MS/MS (134,138).…”