Detection of exogenous and endogenous avian leukosis virus in commercial chicken eggs using reverse transcription and polymerase chain reaction assay

Pham, Thuy D.; Spencer, J. L.; Traina‐Dorge, Vicki; Mullin, David A.; Garry, Robert F.; Johnson, Eric S.

doi:10.1080/03079459994650

Cited by 17 publications

(15 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is worthwhile to note that exceedingly high risk of death from zoonotic bacterial diseases and helminthiasis (up to 100-fold) is being observed in this poultry cohort (manuscript in preparation), thus reenforcing the suggestion of the possible role of transmissible agents in the occurrence of the excess of some of these cancers. Also, it is well established that some of these viruses are present in eggs destined for human consumption as reviewed by us [24] and as reported by us from a survey of eggs sold in supermarkets in the New Orleans metropolitan area [25,26]. Furthermore as mentioned above, we and others have shown that members of the general population have antibodies in their blood against ALSV and REV [27][28][29].…”

Section: Smrmentioning

confidence: 69%

Mortality from malignant diseases—update of the Baltimore union poultry cohort

Johnson

Zhou

Yau

et al. 2009

Cancer Causes Control

Self Cite

View full text Add to dashboard Cite

We previously studied mortality up to 1989 in 2,639 members of a local union who had ever worked in poultry slaughtering and processing plants, because they were exposed to oncogenic viruses present in poultry. In this report, cancer mortality was updated to the year 2003 for 2,580 of the 2,639 subjects who worked exclusively in poultry plants. Mortality in poultry workers was compared with that in the US general population through the estimation of proportional mortality and standardized mortality ratios separately for each race/sex group and for the whole cohort. Compared to the US general population, an excess of cancers of the buccal and nasal cavities and pharynx (base of the tongue, palate and other unspecified mouth, tonsil and oropharynx, nasal cavity/middle ear/accessory sinus), esophagus, recto-sigmoid/rectum/anus, liver and intrabiliary system, myelofibrosis, lymphoid leukemia and multiple myeloma was observed in particular subgroups or in the entire poultry cohort. We hypothesize that oncogenic viruses present in poultry, and exposure to fumes, are candidates for an etiologic role to explain the excess occurrence of at least some of these cancers in the poultry workers. Larger studies which can control for confounding factors are urgently needed to determine the significance of these findings.

show abstract

Section: Smrmentioning

confidence: 69%

Mortality from malignant diseases—update of the Baltimore union poultry cohort

Johnson

Zhou

Yau

et al. 2009

Cancer Causes Control

Self Cite

View full text Add to dashboard Cite

show abstract

“…Another set was PA1 and PA2 which is nondegenerative primer. This set is specific for subgroup A (Pham et al 1999b). …”

Section: Oligonucleotide Primersmentioning

confidence: 98%

“…These viruses are responsible for serious economic losses in the poultry industry worldwide due to reduced growth rate, decreased quantity and quality of egg production, and increased sporadic tumor-induced mortality (Kim and Brown. 2004;Pham et al 1999b). In Iran, veterinary clinicians reported different types of tumor in different organs of chicken and much sporadic mortality in poultry farms (Mohammadi et al 2008; Movassaghi and Mayahi.…”

Section: Introductionmentioning

confidence: 94%

“…So the best way to control ALVs infections is the elimination of infected eggs and chickens from breeding populations in poultry industry (Hatai et al 2008;Hatai et al 2005;Pham et al 1999a). Conventional diagnostic tests for detection of ALV include virus isolation in tissue culture, immunofluorescence assay (IFA), and enzyme-linked immunosorbent assay (ELISA) (Pham et al 1999b). Today virus isolation procedures and IFA identification are not used because these assays are very time consuming and require about 2 weeks for detection of viral infection (Silva et al 2007;Pham et al 1999a).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Detection of avian leukosis virus subgroups in albumen of commercial and native fowl eggs using RT-PCR in Iran

Rajabzadeh

Dadras

Mohammadi

2010

Trop Anim Health Prod

View full text Add to dashboard Cite

Avian leukosis viruses (ALVs) belong to Alpharetrovirus genus of the family Retroviridae that are widespread in nature. Different subgroups of ALV commonly infect egg-laying hens. They are responsible for economic losses due to both mortality and depressed performance in chickens. To investigate the presence of these viruses in chickens in Iran, 560 egg albumens were selected from different farms of Fars province, Iran. These eggs were obtained from flocks of two research centers of native fowl production (60 eggs), a broiler grandparent farm (100 eggs), three broiler breeder farms (300 eggs), and a commercial layer flock (100 eggs). Firstly, for primary screening a degenerative primer set (PU1 and PU2) were used in reverse transcriptase-polymerase chain reaction (RT-PCR). Positive cases were detected in 47 of 300 (15.7%) samples from three broiler breeders, 40 of 100 (40%) samples from commercial layer, 53 of 60 (88.3%) samples from flocks of two research centers of native fowl production, and none from the samples of broiler grandparent. Then RT-PCR was undertaken with primers PA1 and PA2 on the positive samples. RT-PCR analysis detected ALVs in two of 47 (4.3%) samples from three broiler breeders, 13 of 40 (32.5%) samples from commercial layer, and 19 of 53 (35.8%) samples from flocks of two research centers of native fowl production. The sequencing results showed that subgroup E of ALV was the most detected virus among chicken eggs and subgroup B was more prevalent in the eggs of native fowls. This is the first report of the ALV subgroup B and E in egg albumen in Iran.

show abstract

“…The RT-PCR reaction tubes also contained oligonucleotides to amplify 28S rRNA cDNA as an internal control and for standardization purposes. Pham et al (1999) used a RT-PCR to investigate the incidence of avian leukosis virus (ALV) in the eggs of ALV-exposed commercial chickens, and also in eggs randomly chosen from retail stores. To reduce the number of false positives arising from the presence of viral DNA from the cock or hen parents, RNA was extracted from the albumen of unfertilized eggs.…”

Section: Figure 4 Simultaneous Pcr and Pathotyping Of Ndv (Aldous Etmentioning

confidence: 99%

Innovation and discovery: The application of nucleic acid-based technology to avian virus detection and characterization

Cavanagh¹

2001

Avian Pathology

View full text Add to dashboard Cite

Polymerase chain reaction (PCR)-based approaches to the detection, differentiation and characterization of avian pathogens continue to be developed and refined. The PCRs, or reverse transcriptasePCRs, may be general, designed to detect all or most variants of a pathogen, or to be serotype, genotype or pathotype specific. Progress is being made with respect to making nucleic acid approaches more suitable for use in diagnostic laboratories. Robotic workstations are now available for extraction of nucleic acid from many samples in a short time, for routine diagnosis. Following general PCR, the DNA products are commonly analyzed by restriction endonuclease mapping (restriction fragment length polymorphism), using a small number of restriction endonucleases, based on a large body of sequence data. Increasingly, however, nucleotide sequencing is being used to analyze the DNA product, in part due to the expanding use of non-radioactive sequencing methods that are safe and enable high throughout. In this review, I highlight some recent developments with many avian viruses: Newcastle disease virus; circoviruses in canary and pigeon; infectious bursal disease virus (Gumboro disease virus); avian adenoviruses, including Angara disease/infectious hydropericardium virus, haemorrhagic enteritis virus of turkeys, and egg drop syndrome virus; avian herpesviruses, including infectious laryngotracheitis virus, duck plague virus, psittacine herpesvirus (Pacheco's parrot disease virus), Marek's disease virus and herpesvirus of turkeys; avian leukosis virus (associated with lymphoid leukosis or myeloid leukosis, and egg transmission); avian pneumoviruses (turkey rhinotracheitis virus); avian coronaviruses, including infectious bronchitis virus, turkey coronavirus and pheasant coronavirus; astrovirus, in the context of poult enteritis and mortality syndrome, and avian nephritis virus; and avian encephalomyelitis virus, a picornavirus related to hepatitis A virus.

show abstract

Detection of exogenous and endogenous avian leukosis virus in commercial chicken eggs using reverse transcription and polymerase chain reaction assay

Cited by 17 publications

References 38 publications

Mortality from malignant diseases—update of the Baltimore union poultry cohort

Mortality from malignant diseases—update of the Baltimore union poultry cohort

Detection of avian leukosis virus subgroups in albumen of commercial and native fowl eggs using RT-PCR in Iran

Innovation and discovery: The application of nucleic acid-based technology to avian virus detection and characterization

Contact Info

Product

Resources

About