Detection of Extended-Spectrum Beta-Lactamase-Producing and Carbapenem-Resistant Bacteria from Mink Feces and Feed in the United States

Agga, Getahun E.; Silva, Philip J.; Martin, Randal S.

doi:10.1089/fpd.2020.2898

Cited by 12 publications

(6 citation statements)

References 32 publications

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“…Therefore, the use of antibiotics especially PLOS GLOBAL PUBLIC HEALTH from male admitted patients should always be proven by susceptibility testing. The resistant trend observed in this study clearly indicate possible prevalence of extended spectrum beta lactamases (ESBLs) [23], and E. agglomerans, K. pneumonia and S. aureus are clinically important pathogens that can develop multidrug resistance and cause difficult to treat infections in all age groups [24,25]. Imipenem is a carbapenem that shows broad-spectrum activity against gram-positive, gram-negative, and anaerobic bacteria, and is active against cephalosporinresistant Enterobacteriaceae producing ESBLs, whereas piperacillin/tazobactam is the combination of a fourth generation, extended-spectrum penicillin and a beta-lactamase inhibitor that is also effective against β-lactamase producing penicillin-resistant bacterial species [26,27].…”

Section: General Characterization Of Bacterial Isolates From Routine ...mentioning

confidence: 62%

Antibiotic susceptibility patterns of pathogens isolated from laboratory specimens at Livingstone Central Hospital in Zambia

Mwansa

Kamvuma²,

Mulemena³

et al. 2022

PLOS Glob Public Health

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Background Multidrug resistance (MDR) is a global problem that require multifaceted effort to curb it. This study aimed to evaluate the antibiotic susceptibility patterns of routinely isolated bacteria at Livingstone Central Hospital (LCH). Methods A retrospective study was performed on all isolated organisms from patient specimens that were processed from January 2019 to December 2021. Specimens were cultured on standard media and Kirby-Bauer disc diffusion method was employed for susceptibility testing following the Clinical and Laboratory Standard Institute’s recommendations. Results A total of 765 specimens were processed and only 500 (65.4%) met the inclusion criteria. Of the 500, 291(58.2%) specimens were received from female and from the age-group 17–39 years (253, 50.6%) and 40–80 years (145, 29%) in form of blood (331, 66.2%), urine (165, 33%) and sputum (4, 0.8%). Amongst the bacterial isolates, Staphylococcus aureus (142, 28.4%) was the commonest followed by Escherichia coli (91, 18.2%), and Enterobacter agglomerans (76, 15.2%), and Klebsiella pneumoniae (43, 8.6%). The resistance pattern revealed ampicillin (93%) as the least effective drug followed by oxacillin (88%), penicillin (85.6%), co-trimoxazole (81.5%), erythromycin (71.9%), nalidixic acid (68%), and ceftazidime (60%) whereas the most effective antibiotics were imipenem (14.5%), and piperacillin/tazobactam (16.7%). The screening of methicillin resistant Staphylococcus aureus (MRSA) with cefoxitin showed 23.7% (9/38) resistance. Conclusion Increased levels of MDR strains and rising numbers of MRSA strains were detected. Therefore, re-establishing of the empiric therapy is needed for proper patient management, studies to determine the levels of extended spectrum beta lactamase- and carbapenemase-producing bacteria are warranted.

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Section: General Characterization Of Bacterial Isolates From Routine ...mentioning

confidence: 62%

Antibiotic susceptibility patterns of pathogens isolated from laboratory specimens at Livingstone Central Hospital in Zambia

Mwansa

Kamvuma²,

Mulemena³

et al. 2022

PLOS Glob Public Health

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“…However, because the incidence is low and the use of antibiotics is minimal, drug resistance genes are much less likely to spread. A US study reported that 39 out of 42 stool samples contained E. coli carrying CTX-M (93%) (53). In another German prevalence survey on ESBL-producing E. coli, 96.5% of calves in the 10 large rural villages involved were ESBL-positive, 92.9% of which were E. coli (54).…”

Section: Discussionmentioning

confidence: 99%

“…A US study reported that 39 out of 42 stool samples contained E. coli carrying CTX-M (93%) ( 53 ). In another German prevalence survey on ESBL-producing E. coli , 96.5% of calves in the 10 large rural villages involved were ESBL-positive, 92.9% of which were E. coli ( 54 ).…”

Section: Discussionmentioning

confidence: 99%

Prevalence of Multidrug-Resistant CTX-M Extended Spectrum Beta-Lactamase-Producing Escherichia coli From Different Bovine Faeces in China

et al. 2022

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CTX-M extended spectrum beta-lactamase-producing Escherichia coli cause severe health hazards in livestock breeding. To date, little is known about antibiotic resistance differences among bacterial isolates from yaks, cows, and beef cattle; therefore, the aims of this study were to analyse the prevalence of CTX-M-producing E. coli in yak, beef cattle, and dairy cattle feces from different provinces in China. A total of 790 fecal samples from yaks, beef cattle, and dairy cows were used. Among all the samples, 523 non duplicate E. coli isolates were identified, and 29.6% of samples harbored CTX-M producers. The results showed that these E. coli strains harbored 15 clusters of CTX-M genes: CTX-M-79, CTX-M-55, CTX-M-15, CTX-M-14, CTX-M-28, CTX-M-179, CTX-M-65, CTX-M-24, CTX-M-27, CTX-M-102, CTX-M-105, CTX-M-173, CTX-M-238, CTX-M-196, and CTX-M-10. The dominant resistance genes were CTX-M-15, CTX-M-14, and CTX-M-55. Moreover, the distribution of CTX-M genes was related to geographical region. Based on the above findings, we reasoned that bovines are potential reservoirs of antibiotic resistance, and this problem should be given adequate attention.

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“…Therefore, the use of antibiotics especially from male admitted patients should always be proven by susceptibility testing. The resistant trend observed in this study clearly indicate possible prevalence of extended spectrum beta lactamases (ESBLs) (21), and Enterobacter agglomerans, Klebsiella pneumonia and Staphylococcus aureus are clinically important pathogens that can develop multidrug resistance and cause difficult to treat infections in all age groups (22, 23). Imipenem is a carbapenem that shows broad-spectrum activity against gram-positive, gram-negative, and anaerobic bacteria, and is active against cephalosporin-resistant Enterobacteriaceae producing ESBLs, whereas piperacillin/tazobactam is the combination of a fourth generation, extended-spectrum penicillin and a beta-lactamase inhibitor that is also effective against β-lactamase producing penicillin-resistant bacterial species (24, 25).…”

Section: Discussionmentioning

confidence: 79%

Antibiotic susceptibility patterns of pathogens isolated from laboratory specimens at Livingstone Central Hospital in Zambia

Mwansa

Kamvuma

Mulemena

et al. 2022

Preprint

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BackgroundAntibiotics are essential commodities in managing bacterial infections in humans, animals and plants but are hampered by the development of antibiotic resistance which is one of the most serious public health threats of the twenty-first century. Moreover, the rate at which novel antibiotics are discovered is slower that the rate of emerging antibiotic resistance. Therefore, the few remaining potent antibiotics in clinical setting should be safeguarded by closer monitoring of their effectiveness via periodic antibiogram studies. This study aimed to evaluate the antibiotic susceptibility patterns of routinely isolated bacteria at Livingstone Central Hospital (LCH).MethodsA cohort retrospective study with secondary information collected from electronic laboratory system generated reports on all isolated organisms at LCH microbiology laboratory for three years (January 2019 to December 2021) was used. Study variables such as age, gender, patient’s location, name of the organism and the antibiotic susceptibility were considered. Descriptive statistics was used to describe our data and a chi-square test was used for categorical variables where a p-value of ≤0.05 was considered as statistically significant.ResultsA total of 765 specimens were processed from January 2019 to December 2021 and only 500 (65.4%) met the inclusion criteria for this study. Of the 500, 291(58.2%) specimens were received from female and from the age-group 17-39 years (253, 50.6%) and 40-80 years (145, 29%) in form of blood (331, 66.2%), urine (165, 33%) and sputum (4, 0.8%). The out-patient department (323, 64.6%) had a higher number of specimen culture requests that reduced from 175 (35%) for the year 2019 and 2020 to 150 (30%) for year 2021. Amongst the common bacterial isolates identified, Staphylococcus aureus (142, 28.4%) was the commonest isolate followed by Escherichia coli (91, 18.2%), Enterobacter agglomerans (76, 15.2%), and Klebsiella pneumoniae (43, 8.6%). The resistance pattern indicated that ampicillin (93%) was the least effective drug followed by oxacillin (88%), penicillin (85.6%), co-trimoxazole (81.5%), erythromycin (71.9%), nalidixic acid (68%), ceftazidime (60%), tetracycline (55.1%), and ciprofloxacin (45.9%) whereas the most effective antibiotics were imipenem (14.5%), piperacillin/tazobactam (16.7%) and clindamycin (34.5%). The resistance levels were affected by patient gender, location, and specimen type.However, the screening of methicillin resistant Staphylococcus aureus (MRSA) with cefoxitin showed 76.3% (29/38) susceptibility and 23.7% (9/38) resistance.ConclusionThe commonest bacterial isolates were Staphylococcus aureus, Escherichia coli, Enterobacter agglomerans, Klebsiella pneumoniae and Klebsiella oxytoca. The least effective antibiotics were ampicillin, penicillin, oxacillin, cotrimoxazole, and erythromycin whereas the most effective antibiotics were imipenem, piperacillin/tazobactam, and clindamycin. Therefore, re-establishing of the empiric therapy is needed for proper patient management, studies to determine the levels of extended spectrum beta lactamase- and carbapenemase-producing bacteria are warranted.

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Detection of Extended-Spectrum Beta-Lactamase-Producing and Carbapenem-Resistant Bacteria from Mink Feces and Feed in the United States

Cited by 12 publications

References 32 publications

Antibiotic susceptibility patterns of pathogens isolated from laboratory specimens at Livingstone Central Hospital in Zambia

Antibiotic susceptibility patterns of pathogens isolated from laboratory specimens at Livingstone Central Hospital in Zambia

Prevalence of Multidrug-Resistant CTX-M Extended Spectrum Beta-Lactamase-Producing Escherichia coli From Different Bovine Faeces in China

Antibiotic susceptibility patterns of pathogens isolated from laboratory specimens at Livingstone Central Hospital in Zambia

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