Detection of feline calicivirus as norovirus surrogate in food and water sources using filtration and real-time RT-PCR

Cho, Min-Gyu; Jeong, Hye-Mi; Ahn, Junbae; Kim, Kwang-Yup

doi:10.1007/s10068-011-0204-5

Cited by 3 publications

(2 citation statements)

References 24 publications

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“…Second, we use the acidic conditions to enhance the elution to promote the recovery yields from the membrane, no complex reagents and complex procedures. Third, current method was suitable for large volume water sample, which was not suitable for routine monitoring [15,17]. Therefore, our study demonstrates that the approach is a rapid, simple, and efficient way to concentrate NoVs and that the procedure is more sensitive but consumes less time and is less expensive.…”

Section: Discussionmentioning

confidence: 97%

Detection of GI and GII noroviruses in drinking water and vegetables using filtration and real-time RT-PCR

Han

et al. 2014

Eur Food Res Technol

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Section: Discussionmentioning

confidence: 97%

Detection of GI and GII noroviruses in drinking water and vegetables using filtration and real-time RT-PCR

Han

et al. 2014

Eur Food Res Technol

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“…GI and GII groups are more commonly associated with human illness (Zheng et al, 2006) and each group is currently subdivided into 9 and 22 genotypes, respectively (Lu et al, 2015). NoV transmission pathways are the fecal-oral route by consumption of uncooked contaminated food as well as by contact of the person, aerosol and the solid surface (Cho et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Detection and phylogenetic analysis of norovirus from individual septic tanks in the drainage basin of the coastal area located in the Jaran Bay of Korea

Ham¹,

Kim²,

Park³

et al. 2021

Fish Aquat Sci

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Norovirus (NoV) prevalence was investigated in 100 sewage samples collected from 35 individual septic tanks around the drainage basin of Jaran Bay, Korea in January, May, and July of 2017. Genotypes and diversity of NoV strains detected in sewage samples were also assessed using the conventional RT-PCR and phylogenetic analysis. NoV GI or GII were detected in 22 (22.0%) and 24 (24.0%) samples, respectively. Thirteen genotypes were identified with three dominant genotypes (GI.9, GII.5 and GII.17) and GII.17 showed relatively higher prevalence during the survey period. GII.17 strains were clustered into recombinant type variant or NoV GII.17 Kawasaki variant. NoV GII.17 strains were considered emergent epidemic variants with widespread circulation. NoV surveillance strategy should include both environmental (sewage) and clinical data to reveal minor NoV genotypes likely cause of asymptomatic or underreported infections in the local population.

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Quantitative Analysis of Feline Calicivirus Inactivation using Real-time RT-PCR

Jeong¹,

Kim²

2014

Journal of Food Hygiene and Safety

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-Norovirus causes acute gastroenteritis in all age groups and its food poisoning outbreaks are rapidly increasing in Korea. Reverse transcription-polymerase chain reaction (RT-PCR) is most widely used for the rapid detection of foodborne viruses due to high sensitivity. However, the false positive results of RT-PCR obtained against already inactivated viruses could be a serious drawbacks in food safety area. In this study, we investigated a method to yield true positive RT-PCR results only with alive viruses. To decompose the RNA genes from dead viruses, the enzymatic treatments composed of proteinse K and Ribonuclease A were applied to the sanitized and inactivated virus particles. Another aim of this study was to quantify the efficiencies of several major sanitizing treatments using realtime RT-PCR. Feline calicivirus (FCV) that belongs to the same Caliciviridae family with norovirus was used as a surrogate model for norovirus. The initial level of virus in control suspension was approximately 10 4 PFU/mL. Most of inactivated viruses treated with the enzymatic treatment for 30 min at 37 o C were not detected in RT-PCR, Quantification results to verify the inactivation efficiencies of sanitizing treatments using real-time RT-PCR showed no false positive in most cases. We could successfully develope a numerical quantification process for the inactivated viruses after major sanitizing treatments using real-time RT-PCR. The results obtained in this study could provide a novel basis of rapid virus quantification in food safety area.

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Detection of feline calicivirus as norovirus surrogate in food and water sources using filtration and real-time RT-PCR

Cited by 3 publications

References 24 publications

Detection of GI and GII noroviruses in drinking water and vegetables using filtration and real-time RT-PCR

Detection of GI and GII noroviruses in drinking water and vegetables using filtration and real-time RT-PCR

Detection and phylogenetic analysis of norovirus from individual septic tanks in the drainage basin of the coastal area located in the Jaran Bay of Korea

Quantitative Analysis of Feline Calicivirus Inactivation using Real-time RT-PCR

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