Detection of Fibrinogen and Coagulation Factor VIII in Plasma by a Quartz Crystal Microbalance Biosensor

Chen, Yao; Ling, Qu; Fu, Weiling

doi:10.3390/s130606946

Cited by 25 publications

(21 citation statements)

References 26 publications

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“…2). Changes in the viscosity of plasma were caused by the formation of the fibrin clot 14 , and we monitored this significant change in the viscosity by real-time tracking of the phase and amplitude. Before the coagulation measurements we characterized the system in air, Deionized (DI) water and blood plasma.…”

Section: Characterization Of the Systemmentioning

confidence: 99%

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A cartridge based sensor array platform for multiple coagulation measurements from plasma

et al. 2015

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ARTICLE This journal is © The Royal Society of Chemistry 2013Lab Chip, 2014, 00, 1-3 | 1 and Prothrombin Time (PT)). The system has a reader unit and a disposable cartridge. The reader has no electrical connections to the cartridge. This enables simple and low-cost cartridge designs and avoids reliability problems associated with the electrical connections. The cartridge consists of microfluidic channels and MEMS microcantilevers placed in each channel. Microcantilevers are made of electro-plated nickel. They are actuated remotely using an external electro-coil and the read-out is also conducted remotely by a laser. The phase difference between the cantilever oscillation and the coil drive is monitored in real-time. During coagulation, the viscosity of the blood plasma increases resulting in a change in phase read-out. The proposed assay was tested with human and control plasma samples for PT and aPTT measurements. PT and aPTT measurements with using control plasmas are comparable with manufacturer's data sheet and commercial reference device. The measurement system has an overall 7.28 % and 6.33 % CV for PT and aPTT, respectively. For further implementation, the microfluidic channels of the cartridge are functionalized for PT and aPTT tests by drying specific reagents in each channel. Since the simultaneous PT and aPTT measurements are needed in order to properly evaluate the coagulation system, one of the most prominent features of the proposed assay is the enabling parallel measurement of different coagulation parameters. Additionally, the design of the cartridge and the read out system, the obtained reproducible results with 10 µl plasma samples suggesting an opportunity of a possible Point-of -Care application.

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Section: Characterization Of the Systemmentioning

confidence: 99%

“…Magnetoelastic sensors 11,12 , Quartz Crystal Microbalance (QCM) [13][14][15] and Contour-Mode Film Bulk Acoustic Resonator (C-FBAR) based techniques 16 have been utilized to measure coagulation times. However in these studies mixing of the blood samples with the reagents were conducted manually on the sensors.…”

Section: Article Lab On a Chipmentioning

confidence: 99%

A cartridge based sensor array platform for multiple coagulation measurements from plasma

et al. 2015

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“…When the coagulation is initialized, thrombin acts on fibrinogens which results in the fibrin generation, then fibrins aggregate and form insoluble clots. Changes in the viscosity of plasma were caused by the formation of the fibrin clot [5]. This significant change in the viscosity can be monitored by realtime tracking of the phase and amplitude.…”

Section: Methodsmentioning

confidence: 99%

“…The researchers utilized microfluidic platforms [3], Quartz Crystal Microbalance (QCM) [4,5] Film Bulk Acoustic Resonator (FBAR) [6] based techniques for clot-time measurements. But they are limited to single tests and not multiplexed.…”

Section: Introductionmentioning

confidence: 99%

LoC sensor array platform for real-time coagulation measurements

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et al. 2014

2014 IEEE 27th International Conference on Micro Electro Mechanical Systems (MEMS)

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1This paper reports a MEMS-based sensor array enabling multiple clot-time tests in one disposable microfluidic cartridge using plasma. The versatile LoC (Lab-on-Chip) platform technology is demonstrated here for real-time coagulation tests (activated Partial Thrompoblastin Time (aPTT) and Prothrombin Time (PT)). The system has a reader unit and a disposable cartridge. The reader has no electrical connections to the cartridge, which consists of multiple microfluidic channels and MEMS microcantilevers placed in each channel. Microcantilevers are made of electro-plated nickel and actuated remotely using an external electro-coil. The read-out is also conducted remotely by a laser and the phase of the MEMS oscillator is monitored real-time. The system is capable of monitoring coagulation time with a precision estimated at 0.1sec.

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“…Currently, there are many biomedical applications using QCR's as transducers. For example: blood coagulation analysis [14][15][16][17][18], Alzheimer disease [19,20], Cancer [21][22][23][24] and pathogen detection [25][26][27][28]. Also, many environmental monitoring applications with QCR's.…”

Section: Introductionmentioning

confidence: 99%

Diseño y evaluación de un biosensor basado en resonadores de cristal de cuarzo (QCR) para caracterizar muestras biológicas relacionadas con enfermedades artríticas

Ahumada¹,

Armando²

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IVResumen de la tesis de Luis Armando Carvajal Ahumada, presentada como requisito parcial para la obtención del grado de DOCTOR en INGENIERÍA BIOMÉDICA. Madrid, 03 de Abril de 2017 DISEÑO Y EVALUACIÓN DE UN SENSOR BASADO EN RESONADORES DE CRISTAL DE CUARZO (QCR) PARA CARACTERIZAR MUESTRAS BIOLÓGICAS RELACIONADAS CON LA DIAGNOSIS DE ENFERMEDADES ARTRÍTICASResumen:Los resonadores de cristal de cuarzo son dispositivos ampliamente utilizados como biosensores debido a su alta sensibilidad ante fenómenos físico-químicos que ocurren en su superficie, a la reproducibilidad de las mediciones y por ser transductores fáciles de utilizar. Sauerbrey[1] fue el primero en demostrar que el resonador cristal de cuarzo puede ser utilizado como una microbalanza útil para caracterizar pequeñas deposiciones de masa en el electrodo del cristal. Sauerbrey demostró que, si se deposita una delgada capa de material rígido sobre el cristal de cuarzo, tanto el cristal como el material depositado vibrarán a la misma frecuencia y se generará un cambio en la frecuencia de resonancia inicial del cristal. Específicamente, la frecuencia de resonancia disminuye al aumentar la cantidad de masa depositada.El cristal de cuarzo también puede operar en medio fluidos. En este caso además del desplazamiento en frecuencia del cristal, se genera una pérdida significativa en el factor de calidad del resonador. Para fluidos Newtonianos (viscosidad constante), Kanazawa y Gordon[2] demostraron que el desplazamiento en frecuencia que sufre el resonador así como el cambio en el ancho de la curva de admitancia, depende de la densidad y la viscosidad del líquido. Este fenómeno permite utilizar los resonadores de cristal de cuarzo como pequeños viscosímetros siempre que se conozca la densidad del fluido a analizar.En el caso de deposición de fluidos viscoelásticos, la medición del cambio en la frecuencia de resonancia del cristal no es suficiente para obtener el valor de viscosidad del fluido. Por tanto, se requiere de la medición de una segunda variable (mitad del ancho de media banda -Γ) para obtener el valor correcto de la viscosidad del fluido depositado.[3] Para fluidos pseudoplásticos (viscosidad aparente dependiente de la velocidad de cizallamiento aplicada), se requiere de un modelo matemático que explique el comportamiento de la viscosidad del fluido a distintas velocidades de cizallamiento. En particular para el análisis de muestras con este comportamiento, se ha elegido el modelo Rouse ya que explica con suficiente detalle el comportamiento pseudoplástico y resulta simple de aplicar a las medidas experimentales.En este trabajo se propone el diseño de un biosensor basado en un resonador de cristal de cuarzo (QCR) para la caracterización de muestras biológicas que emulan el comportamiento del líquido sinovial.El líquido sinovial es una mezcla de plasma dializado y ácido hialurónico con comportamiento pseudoplástico. Su principal función es la de actuar como lubricante en las articulaciones V manteniendo al mínimo la fricción entre los huesos, especialment...

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Detection of Fibrinogen and Coagulation Factor VIII in Plasma by a Quartz Crystal Microbalance Biosensor

Cited by 25 publications

References 26 publications

A cartridge based sensor array platform for multiple coagulation measurements from plasma

A cartridge based sensor array platform for multiple coagulation measurements from plasma

LoC sensor array platform for real-time coagulation measurements

Diseño y evaluación de un biosensor basado en resonadores de cristal de cuarzo (QCR) para caracterizar muestras biológicas relacionadas con enfermedades artríticas

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