Detection of filarial parasites in domestic cats by PCR-RFLP of ITS1

Nuchprayoon, Surang; Junpee, Alisa; Nithiuthai, Suwannee; Chungpivat, Sudchit; Suvannadabba, Saravudh; Poovorawan, Yong

doi:10.1016/j.vetpar.2006.04.003

Cited by 35 publications

(23 citation statements)

References 9 publications

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“…Therefore, since our isolates have a 4% nucleotide difference from the reported D. repens sequence and an 11% difference from the D. immitis sequence, they belong to a separate species. The 18S-ITS1-5.8S gene cluster has also been employed to differentiate between filariae at the genus level (18,19). A novel species, Acanthocheilonema ladakhii, was described based on the 18S ribosomal DNA (rDNA) gene sequence (15).…”

Section: Discussionmentioning

confidence: 99%

A Novel Dirofilaria Species Causing Human and Canine Infections in Hong Kong

Wong

Poon

et al. 2012

J Clin Microbiol

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Dirofilariasis is globally the commonest manifestation of zoonotic filariasis. We report the detection of a novel canine species causing human and canine dirofilariasis in Hong Kong. Three human cases occurring over 10 months were identified, one presenting with cervical lymphadenopathy, one with an abdominal subcutaneous mass, and one with a subconjunctival nodule. Transected worms recovered from the resected abdominal subcutaneous mass were morphologically compatible with Dirofilaria. The cox1 gene sequences of the three human isolates were identical; however, they were only 96.2% and 89.

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Section: Discussionmentioning

confidence: 99%

A Novel Dirofilaria Species Causing Human and Canine Infections in Hong Kong

Wong

Poon

et al. 2012

J Clin Microbiol

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“…Molecular methods have been proposed for differentiating between different species of filarids, with trustworthy results (Gasser et al 1996;Watts et al 1999;Mar et al 2002;Nuchprayoon et al 2005Nuchprayoon et al , 2006Rishniw et al 2006).…”

Section: Introductionmentioning

confidence: 99%

Detection of dog filariasis in Marajo Island, Brazil by classical and molecular methods

et al. 2009

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Canine filariasis in domestic and wild dogs, foxes, and wolves is caused by several species of filarids. Although these filarial species inhabit different loci in the vertebrate definitive hosts, they generally release microfilariae into the bloodstream. Data about filarial infection in dogs in Brazil, especially on the Marajo Island, is scarce. For this reason, we conducted an analysis of 188 domestic dogs within two Marajo Island municipalities. The overall prevalence of microfilaremic was 32.45%; taken by blood smear and modified Knott's method. No significant difference of positivity between male and female was observed (X(2) Yates's correction = 0.341; p = 0.559). Significant age-infection ratios were detected (X(2) = 32.943; p < 0.0001). A high occult infection was detected (53.84%). PCR of rDNA and phylogenetic tree indicated that the microfilariae and adult worms found in domestic dogs from Marajo Island were Dirofilaria immitis.

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“…Restriction digestion is as a matter of routine using for the determination of the species identification of closely related parasitic and plant parasitic nematodes. Results indicate the usefulness of these markers for diagnostic purposes, and epidemiologic and molecular-systematic studies on parasites and other eukaryotic organisms (Conole et al 1999;Braasch et al 1999;Nuchprayoon et al 2006;De Silva et al 2006). In conclusion, the populations undergoing arrested development and without ability of undergoing arrested development of T. colubriformis can be distinguished by using PCR amplification of cistron rDNA, its digestion with Hinc II restriction endonuclease, and separation of the restriction products by polyacrylamide gel electrophoresis or agarose gel.…”

Section: Resultsmentioning

confidence: 91%

Trichostrongylus colubriformis rDNA polymorphism associated with arrested development

et al. 2008

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Polymerase chain reaction-restriction fragment length polymorphism) was performed on the cistron of rDNA in the two groups of infective larvae Trichostrongylus colubriformis-the population with and without ability to undergo arrested development. General primers designed by Caenorhabditis elegans rDNA were used for the amplification of the rDNA cistron between genes 18S and 28S. Amplified fragments were digested by using a series of restriction endonucleases. Hinc II restriction profiles unique for each T. colubriformis populations were observed, and therefore enzyme Hinc II appears to be useful for the determination of populations with and without the ability to undergo arrested development. Molecular markers of arrested development ability have not been studied on this part of rDNA before.

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Detection of filarial parasites in domestic cats by PCR-RFLP of ITS1

Cited by 35 publications

References 9 publications

A Novel Dirofilaria Species Causing Human and Canine Infections in Hong Kong

A Novel Dirofilaria Species Causing Human and Canine Infections in Hong Kong

Detection of dog filariasis in Marajo Island, Brazil by classical and molecular methods

Trichostrongylus colubriformis rDNA polymorphism associated with arrested development

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