Detection of foliar endophytes and their metabolites in Picea and Pinus seedling needles

McMullin, David; Nguyen, Hai D. T.; Daly, Grace; Ménard, Bertrand; Miller, J. David

doi:10.1016/j.funeco.2017.09.003

Cited by 23 publications

(14 citation statements)

References 31 publications

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“…Further analysis revealed that homodimeric macrolide pyrenophorol present in the extracts was responsible for the growth inhibition of the pine pathogen Cronartium ribicola J.C.Fisch. Other authors [198] have shown that the antifungal compounds griseofulvin (from endophyte Xylaria sp.) and pyrenophorol (from L. nitens) were present in sufficient amounts to affect conifer needle diseases including white pine blister rust C. ribicola [198].…”

Section: Aerial Endophytes and Tree Healthmentioning

confidence: 99%

“…Other authors [198] have shown that the antifungal compounds griseofulvin (from endophyte Xylaria sp.) and pyrenophorol (from L. nitens) were present in sufficient amounts to affect conifer needle diseases including white pine blister rust C. ribicola [198]. These results support the hypothesis that several metabolites with antifungal properties secreted by fungal endophytes may enhance the tolerance of the host tree to fungal pathogens and herbivory [198][199][200][201][202][203].…”

Section: Aerial Endophytes and Tree Healthmentioning

confidence: 99%

“…Most importantly, it was shown that growth of C. fumiferana and damage to the foliage of Picea glauca (Moench) Voss were either reduced or mitigated using low concentrations of the anti-insect compounds vermiculine (from endophyte Phialocephala sp.) and rugulosin (P. scopiformis) [198].…”

Section: Aerial Endophytes and Tree Healthmentioning

confidence: 99%

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Forest Tree Microbiomes and Associated Fungal Endophytes: Functional Roles and Impact on Forest Health

Terhonen

Blumenstein

Kovalchuk

et al. 2019

Forests

171

113

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Terrestrial plants including forest trees are generally known to live in close association with microbial organisms. The inherent features of this close association can be commensalism, parasitism or mutualism. The term “microbiota” has been used to describe this ecological community of plant-associated pathogenic, mutualistic, endophytic and commensal microorganisms. Many of these microbiota inhabiting forest trees could have a potential impact on the health of, and disease progression in, forest biomes. Comparatively, studies on forest tree microbiomes and their roles in mutualism and disease lag far behind parallel work on crop and human microbiome projects. Very recently, our understanding of plant and tree microbiomes has been enriched due to novel technological advances using metabarcoding, metagenomics, metatranscriptomics and metaproteomics approaches. In addition, the availability of massive DNA databases (e.g., NCBI (USA), EMBL (Europe), DDBJ (Japan), UNITE (Estonia)) as well as powerful computational and bioinformatics tools has helped to facilitate data mining by researchers across diverse disciplines. Available data demonstrate that plant phyllosphere bacterial communities are dominated by members of only a few phyla (Proteobacteria, Actinobacteria, Bacteroidetes). In bulk forest soil, the dominant fungal group is Basidiomycota, whereas Ascomycota is the most prevalent group within plant tissues. The current challenge, however, is how to harness and link the acquired knowledge on microbiomes for translational forest management. Among tree-associated microorganisms, endophytic fungal biota are attracting a lot of attention for their beneficial health- and growth-promoting effects, and were preferentially discussed in this review.

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Section: Aerial Endophytes and Tree Healthmentioning

confidence: 99%

Section: Aerial Endophytes and Tree Healthmentioning

confidence: 99%

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Forest Tree Microbiomes and Associated Fungal Endophytes: Functional Roles and Impact on Forest Health

Terhonen

Blumenstein

Kovalchuk

et al. 2019

Forests

171

113

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“…1), along with piliformic acid 38 . Subsequent investigations of white pine seedlings infected with this Xylaria species found griseofulvin at biologically effective concentrations in the needles 69 .…”

Section: Discussionmentioning

confidence: 98%

Metabolomic-guided discovery of cyclic nonribosomal peptides from Xylaria ellisii sp. nov., a leaf and stem endophyte of Vaccinium angustifolium

Ibrahim

Tanney

Fei

et al. 2020

Sci Rep

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fungal endophytes are sources of novel bioactive compounds but relatively few agriculturally important fruiting plants harboring endophytes have been carefully studied. Previously, we identified a griseofulvin-producing Xylaria species isolated from Vaccinium angustifolium, V. corymbosum, and Pinus strobus. Morphological and genomic analysis determined that it was a new species, described here as Xylaria ellisii. Untargeted high-resolution LC-MS metabolomic analysis of the extracted filtrates and mycelium from 15 blueberry isolates of this endophyte revealed differences in their metabolite profiles. toxicity screening of the extracts showed that bioactivity was not linked to production of griseofulvin, indicating this species was making additional bioactive compounds. Multivariate statistical analysis of Lc-MS data was used to identify key outlier features in the spectra. this allowed potentially new compounds to be targeted for isolation and characterization. this approach resulted in the discovery of eight new proline-containing cyclic nonribosomal peptides, which we have given the trivial names ellisiiamides A-H. Three of these peptides were purified and their structures elucidated by one and twodimensional nuclear magnetic resonance spectroscopy (1D and 2D NMR) and high-resolution tandem mass spectrometry (HRMS/MS) analysis. The remaining five new compounds were identified and annotated by high-resolution mass spectrometry. ellisiiamide A demonstrated Gram-negative activity against Escherichia coli BW25113, which is the first reported for this scaffold. Additionally, several known natural products including griseofulvin, dechlorogriseofulvin, epoxy/cytochalasin D, zygosporin E, hirsutatin A, cyclic pentapeptides #1-2 and xylariotide A were also characterized from this species.Vaccinium angustifolium (wild lowbush blueberries or commonly wild blueberries) were consumed fresh and preserved for the winter by the Indigenous peoples of northeastern North America and rapidly incorporated into the diets of European settlers in Canada from the early 17 th century 1,2 . Today, blueberries comprise more than half of all fruit production in Canada. Wild blueberries often grow in forests where Pinus strobus (eastern white pine) is the dominant tree species. Eastern white pine is an economically, ecologically, and culturally important keystone tree species in eastern N. American forests, especially for bird species 3,4 .Endophytes are an ecological category of phylogenetically diverse fungi that can asymptomatically colonize healthy plant tissues. Ascomycetous endophytes of various species of Vaccinium have been reported over the past three decades. This includes from surface-sterilized tissues of Vaccinium vitis-idaea (lingonberry, European open Scientific RepoRtS | (2020) 10:4599 | https://doi.org/10.1038/s41598-020-61088-xwww.nature.com/scientificreports www.nature.com/scientificreports/ blueberry) and V. myrtillus (bilberry, whortleberry) in Europe 5 , V. dunalianum var. urophyllum (South China blueberry) in China 6 ,...

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“…When living inside plants, some endophytes act as mutualistic symbionts, assisting plant functioning, survival and fitness (Carroll, 1988;Schulz and Boyle, 2005;Rodríguez et al, 2009;Hardoim et al, 2015;Vandenkoornhuyse et al, 2015). Fungal endophytes have received considerable attention in recent years because of their impact on plant fitness and plant responses to biotic and abiotic stressors (Singh et al, 2011;Lata et al, 2018;Rabiey et al, 2019), and for being a potential source of bioactive compounds (Richardson et al, 2015;McMullin et al, 2018;Tanney et al, 2018). The role of fungal endophytes in plant resilience is especially important in trees, which face extreme weather conditions and recurring pathogen and insect attacks during their long lifespan (Lau et al, 2017;Terhonen et al, 2019, Martínez-Arias et al, 2020.…”

Section: Introductionmentioning

confidence: 99%

Endophyte inoculation enhancesUlmus minorresistance to Dutch elm disease

Martínez‐Arias

Sobrino-Plata

Ormeño-Moncalvillo

et al. 2020

Preprint

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Certain fungal endophytes are known to improve plant resistance to biotic stresses in forest trees. In this study, three stem fungal endophytes belonging to classes Cystobasidiomycetes, Eurotiomycetes and Dothideomycetes were selected among 210 isolates for their potential as enhancers of Ulmus minor resistance to Ophiostoma novo-ulmi. We evaluated phenotypic traits of these endophytes that could be beneficial for O. novo-ulmi inhibition in the host plant. Under in vitro conditions, the Dothideomycetous isolate YCB36 strongly inhibited O. novo-ulmi growth, released antipathogen VOCs, chitinases and siderophores, and overlapped with the pathogen in nutrient utilization patterns. These functional traits could explain the 40% reduction in leaf wilting due to O. novo-ulmi in elm trees pre-inoculated with this endophyte. Ulmus minor trees responded to endophyte inoculation with increased stomatal conductance and higher amounts of flavonoids and total phenolic compounds in xylem tissues, suggesting induction of defence metabolism.

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Detection of foliar endophytes and their metabolites in Picea and Pinus seedling needles

Cited by 23 publications

References 31 publications

Forest Tree Microbiomes and Associated Fungal Endophytes: Functional Roles and Impact on Forest Health

Forest Tree Microbiomes and Associated Fungal Endophytes: Functional Roles and Impact on Forest Health

Metabolomic-guided discovery of cyclic nonribosomal peptides from Xylaria ellisii sp. nov., a leaf and stem endophyte of Vaccinium angustifolium

Endophyte inoculation enhancesUlmus minorresistance to Dutch elm disease

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