Detection of foot-and-mouth disease virus in the breath of infected cattle using a hand-held device to collect aerosols

Christensen, Laurids Siig; Brehm, Katharina Erika Gerda; Skov, Julia; Harlow, Kenneth W.; Christensen, J.A.; Haas, Bernd

doi:10.1016/j.jviromet.2011.06.011

Cited by 24 publications

(18 citation statements)

References 15 publications

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“…Planned future research will include more rigorous air sampling as these results suggest that air sampling shows great promise as a predictor of transmission and may also prove to be useful to detect preclinical infection. Hand held devices have been developed and their feasibility for monitoring shedding of FMDV in cattle is being tested [42]. …”

Section: Discussionmentioning

confidence: 99%

Understanding foot-and-mouth disease virus transmission biology: identification of the indicators of infectiousness

Chase-Topping

Handel

Bankowski

et al. 2013

Vet Res

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The control of foot-and-mouth disease virus (FMDV) outbreaks in non-endemic countries relies on the rapid detection and removal of infected animals. In this paper we use the observed relationship between the onset of clinical signs and direct contact transmission of FMDV to identify predictors for the onset of clinical signs and identify possible approaches to preclinical screening in the field. Threshold levels for various virological and immunological variables were determined using Receiver Operating Characteristic (ROC) curve analysis and then tested using generalized linear mixed models to determine their ability to predict the onset of clinical signs. In addition, concordance statistics between qualitative real time PCR test results and virus isolation results were evaluated. For the majority of animals (71%), the onset of clinical signs occurred 3–4 days post infection. The onset of clinical signs was associated with high levels of virus in the blood, oropharyngeal fluid and nasal fluid. Virus is first detectable in the oropharyngeal fluid, but detection of virus in the blood and nasal fluid may also be good candidates for preclinical indicators. Detection of virus in the air was also significantly associated with transmission. This study is the first to identify statistically significant indicators of infectiousness for FMDV at defined time periods during disease progression in a natural host species. Identifying factors associated with infectiousness will advance our understanding of transmission mechanisms and refine intra-herd and inter-herd disease transmission models.

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Section: Discussionmentioning

confidence: 99%

Understanding foot-and-mouth disease virus transmission biology: identification of the indicators of infectiousness

Chase-Topping

Handel

Bankowski

et al. 2013

Vet Res

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“…In previous studies, human influenza A viruses were detected in exhaled breath using EBC [19,20] as well as filter [21], mask [22,23] and a liquid sampler [24]. In another study, foot-and-mouth disease viruses were also found in the exhaled air from experimentally infected cattle [25]. In addition, high levels of bacterial concentrations in EBC were also observed in other studies [26][27][28][29].…”

Section: Introductionmentioning

confidence: 68%

Molecular and Microscopic Analysis of Bacteria and Viruses in Exhaled Breath Collected Using a Simple Impaction and Condensing Method

Shen

et al. 2012

PLoS ONE

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Exhaled breath condensate (EBC) is increasingly being used as a non-invasive method for disease diagnosis and environmental exposure assessment. By using hydrophobic surface, ice, and droplet scavenging, a simple impaction and condensing based collection method is reported here. Human subjects were recruited to exhale toward the device for 1, 2, 3, and 4 min. The exhaled breath quickly formed into tiny droplets on the hydrophobic surface, which were subsequently scavenged into a 10 µL rolling deionized water droplet. The collected EBC was further analyzed using culturing, DNA stain, Scanning Electron Microscope (SEM), polymerase chain reaction (PCR) and colorimetry (VITEK 2) for bacteria and viruses.Experimental data revealed that bacteria and viruses in EBC can be rapidly collected using the method developed here, with an observed efficiency of 100 µL EBC within 1 min. Culturing, DNA stain, SEM, and qPCR methods all detected high bacterial concentrations up to 7000 CFU/m3 in exhaled breath, including both viable and dead cells of various types. Sphingomonas paucimobilis and Kocuria variants were found dominant in EBC samples using VITEK 2 system. SEM images revealed that most bacteria in exhaled breath are detected in the size range of 0.5–1.0 µm, which is able to enable them to remain airborne for a longer time, thus presenting a risk for airborne transmission of potential diseases. Using qPCR, influenza A H3N2 viruses were also detected in one EBC sample. Different from other devices restricted solely to condensation, the developed method can be easily achieved both by impaction and condensation in a laboratory and could impact current practice of EBC collection. Nonetheless, the reported work is a proof-of-concept demonstration, and its performance in non-invasive disease diagnosis such as bacterimia and virus infections needs to be further validated including effects of its influencing matrix.

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“…In cattle, analysis of VOCs has been explored to diagnose bovine respiratory disease (Burciaga-Robles et al, 2009), brucellosis (Knobloch et al, 2009), bovine tuberculosis (Fend et al, 2005; Peled et al, 2012), Johne's disease (Kumanan et al, 2009), ketoacidosis (Mottram et al, 1999), and normal rumen physiology. A rapid, non-invasive identification of foot-and-mouth disease has been performed using air samples collected with a hand-held prototype device equipped with electrostatic particle capture in a microchip chamber of 10-15 μL (Christensen et al, 2011).…”

Section: Biosensors For Breath Analysismentioning

confidence: 99%

“…In this case, infectious viruses are captured and subjected to analysis by real-time PCR (polymerase chain reaction). Such biosensors can hasten the process of monitoring, diagnosis and isolation of contaminated livestock in epidemiological contingencies (Christensen et al, 2011; Wilson, 2015).…”

Section: Introductionmentioning

confidence: 99%

Recent Advancement in Biosensors Technology for Animal and Livestock Health Management

Neethirajan

Huang

Tuteja

et al. 2017

Preprint

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Detection of foot-and-mouth disease virus in the breath of infected cattle using a hand-held device to collect aerosols

Cited by 24 publications

References 15 publications

Understanding foot-and-mouth disease virus transmission biology: identification of the indicators of infectiousness

Understanding foot-and-mouth disease virus transmission biology: identification of the indicators of infectiousness

Molecular and Microscopic Analysis of Bacteria and Viruses in Exhaled Breath Collected Using a Simple Impaction and Condensing Method

Recent Advancement in Biosensors Technology for Animal and Livestock Health Management

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