Detection of Fowlpox virus carrying distinct genome segments of Reticuloendotheliosis virus

Joshi, Lok R.; Bauermann, Fernando V.; Hain, Kyle S.; Kutish, G. F.; Armién, Aníbal G.; Lehman, Chad P.; Neiger, Regg D.; Afonso, Claudio L.; Tripathy, Deoki N.; Diel, Diego G.

doi:10.1016/j.virusres.2018.10.017

Cited by 26 publications

(22 citation statements)

References 20 publications

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“…231,521 to 321,540 and reverse primer that annealed to REV 5 LTR at nucleotide no. 231,874 to 231,891 of the reference APV MH719203/TKPV SD15-670.1 isolate [32]. Both PCR protocols were done with the extracted DNA from separate pock lesions homogenates of 40 tested isolates (10 FWPV, 10 PGPV, 10 TKPV, and 10 CNPV).…”

Section: Polymerase Chain Reactionmentioning

confidence: 99%

“…Importantly, the obtained REV-5 LTR sequences (370 bp) from mans17F, mans17T, and mans17C isolates were aligned with the full-genome sequence of the MH719203/TKPV SD15-670.1 isolate as a reference APV [32], using the NCBI BLAST tool that was used to detect the insertion site of REV in the APV genomes. The results revealed that the amplified 370-bp product falls between nucleotide no.…”

Section: Sequencing and Phylogenetic Analysis Of The Apv P4b Gene Andmentioning

confidence: 99%

“…It is noteworthy to state that several previous studies across the world detected REV sequences integrated in the genomes of field and vaccine strains of FWPV, PGPV, and TKPV [16,17,[31][32][33][34][35][36][37][38][39][40][41][42][43]. Still, no REV full or partial LTRs have never seen integrated in canarypox viruses (CNPVs) [16,17,[31][32][33][34][35][36][37].…”

Section: Introductionmentioning

confidence: 99%

“…It has been reported that the FWPV vaccines are the cause of REV provirus retention in fowls, but some researchers disagree with this hypothesis, since REV integration was detected in the FWPV genome before the wide spread of the FWPV vaccine in poultry [ 30 ]. It is noteworthy to state that several previous studies across the world detected REV sequences integrated in the genomes of field and vaccine strains of FWPV, PGPV, and TKPV [ 16 , 17 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 ]. Still, no REV full or partial LTRs have never seen integrated in canarypox viruses (CNPVs) [ 16 , 17 , 31 , 32 , 33 , 34 , 35 , 36 , 37 ].…”

Section: Introductionmentioning

confidence: 99%

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Molecular Detection of Reticuloendotheliosis Virus 5′ Long Terminal Repeat Integration in the Genome of Avipoxvirus Field Strains from Different Avian Species in Egypt

Mosad

El‐Tholoth

El-Kenawy

et al. 2020

Biology

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Avipoxviruses (APVs) are among the most complex viruses that infect a wide range of birds’ species. The infection by APVs is often associated with breathing and swallowing difficulties, reduced growth, decreased egg production, and high mortalities in domestic poultry. In the present study, 200 cutaneous nodular samples were collected from different avian species (chicken, pigeon, turkey, and canary) suspected to be infected with APVs from Dakahlia Governorate, Egypt. Pooled samples (n = 40) were prepared and inoculated in embryonated chicken eggs (ECEs). APVs were then identified by polymerase chain reaction (PCR) and sequence analysis of the APV P4b gene. Furthermore, the forty strains of APVs were screened for the presence of reticuloendotheliosis virus (REV)-5’LTR in their genomes. Interestingly, the phylogenic tree of the APV P4b gene was separated into 2 clades: clade 1, in which our fowlpox virus (FWPV), turkeypox virus (TKPV), and canarypox virus (CNPV) isolates were grouped, along with reference FWPVs and TKPVs retrieved from GenBank, whereas, in clade2, the pigeonpox virus (PGPV) isolate was grouped with PGPVs retrieved from GenBank. Likewise, REV-5’LTR was amplified from 30 strains isolated from chicken, turkey, and canary, while PGPV strains were free from REV-5’LTR integration. To the best of our knowledge, this study involved the detection and characterization of REV-5’LTR insertions in the APVs field isolates in Egypt for the first time. Given the above information, further future research seems recommended to understand the impact of the resulting REV-5’LTR insertions on the pathogenesis, virulence, and inadequate vaccine protection against APVs.

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Section: Polymerase Chain Reactionmentioning

confidence: 99%

Section: Sequencing and Phylogenetic Analysis Of The Apv P4b Gene Andmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Molecular Detection of Reticuloendotheliosis Virus 5′ Long Terminal Repeat Integration in the Genome of Avipoxvirus Field Strains from Different Avian Species in Egypt

Mosad

El‐Tholoth

El-Kenawy

et al. 2020

Biology

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“…REV is an avian retrovirus that causes lymphocyte tumorigenesis, immunosuppression, growth retardation and runting syndrome in chickens and turkeys [4]. REV may be transmitted horizontally by direct contact between birds and indirectly by mosquitos and contaminated vaccines against fowlpox [14][15][16], Gallid herpesvirus 2 [17,18] and MD [19]. Serologic studies have confirmed that reticuloendotheliosis viral infection is common in commercial layer, broiler, and turkey flocks worldwide [20][21][22].…”

Section: Introductionmentioning

confidence: 99%

Gut microbiota profiles of commercial laying hens infected with tumorigenic viruses

Wan

Sun

et al. 2020

BMC Vet Res

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Background: Studies have shown that some viral infections cause structural changes in the intestinal microflora, but little is known about the effects of tumorigenic viral infection on the intestinal microflora of chickens. Results: A 29-week commercial layer flock positive for avian leukosis virus-J (ALV-J), Marek's disease virus (MDV) and avian reticuloendotheliosis virus (REV) was selected, and fresh fecal samples were collected and examined for the composition of the gut microflora by Illumina sequencing of the V3-V4 region of the 16S rRNA gene. The operational taxonomic units (OTUs) of the fecal microbiota differentiated the chickens infected with only ALV-J and those coinfected with ALV-J and MDV or REV from infection-negative chickens. The enrichment and diversity of cloacal microflora in chickens infected with ALV-J alone were slightly different from those in the infection-negative chickens. However, the diversity of cloacal microflora was significantly increased in chickens coinfected with both ALV-J and MDV or REV. Conclusions: The intestinal microbiota was more strongly disturbed in chickens after coinfection with ALV-J and MDV or REV than after infection with ALV-J alone, and there may be underlying mechanisms by which the capacity for the stabilization of the intestinal flora was impaired due to viral infection and tumorigenesis.

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Characterization of Iranian canarypox and pigeonpox virus strains

et al. 2019

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Detection of Fowlpox virus carrying distinct genome segments of Reticuloendotheliosis virus

Cited by 26 publications

References 20 publications

Molecular Detection of Reticuloendotheliosis Virus 5′ Long Terminal Repeat Integration in the Genome of Avipoxvirus Field Strains from Different Avian Species in Egypt

Molecular Detection of Reticuloendotheliosis Virus 5′ Long Terminal Repeat Integration in the Genome of Avipoxvirus Field Strains from Different Avian Species in Egypt

Gut microbiota profiles of commercial laying hens infected with tumorigenic viruses

Characterization of Iranian canarypox and pigeonpox virus strains

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