Detection of free prostate specific antigen (fPSA) on a nanoporous gold platform

Shulga, Olga V.; Zhou, Dan; Demchenko, Alexei V.; Stine, Keith J.

doi:10.1039/b712760j

Cited by 59 publications

(57 citation statements)

References 25 publications

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“…These SAMs can act as simple modifiers [68] or be used as linkers to attach various molecules to its surface such as proteins, enzymes, antibodies, or photosystem I [65,66,90,[106][107][108][109]. Because of the very high surface areas of nanoporous gold, a significantly larger amount of the modifier can be immobilized on its surface relative to a planar gold electrode.…”

Section: Specific Features Of Nanoporous Goldmentioning

confidence: 99%

Nanoporous Gold Electrodes and Their Applications in Analytical Chemistry

Collinson

2013

ISRN Analytical Chemistry

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Nanoporous gold prepared by dealloying Au:Ag alloys has recently become an attractive material in the field of analytical chemistry. This conductive material has an open, 3D porous framework consisting of nanosized pores and ligaments with surface areas that are 10s to 100s of times larger than planar gold of an equivalent geometric area. The high surface area coupled with an open pore network makes nanoporous gold an ideal support for the development of chemical sensors. Important attributes include conductivity, high surface area, ease of preparation and modification, tunable pore size, and a bicontinuous open pore network. In this paper, the fabrication, characterization, and applications of nanoporous gold in chemical sensing are reviewed specifically as they relate to the development of immunosensors, enzyme-based biosensors, DNA sensors, Raman sensors, and small molecule sensors.

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Section: Specific Features Of Nanoporous Goldmentioning

confidence: 99%

Nanoporous Gold Electrodes and Their Applications in Analytical Chemistry

Collinson

2013

ISRN Analytical Chemistry

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“…Np-Au benefits from several desirable attributes, including large effective surface area, high electrical conductivity, well-established surface functionalization techniques, and biocompatibility 9 . Even though there has been a rapid expansion of studies on np-Au, most of them focus on np-Au's mechanical properties 10,11 , catalytic activity 12 , and biomolecular sensing performance [13][14][15] . While the desirable attributes are highly useful for several biomedical tools 16,17 , the applications in this area have been limited.…”

Section: Introductionmentioning

confidence: 99%

Microfabrication of Nanoporous Gold Patterns for Cell-material Interaction Studies

Daggumati

Kurtuluş

Dimlioglu

et al. 2013

JoVE

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Nanostructured materials with feature sizes in tens of nanometers have enhanced the performance of several technologies, including fuel cells, biosensors, biomedical device coatings, and drug delivery tools. Nanoporous gold (np-Au), produced by a nano-scale self-assembly process, is a relatively new material that exhibits large effective surface area, high electrical conductivity, and catalytic activity. These properties have made np-Au an attractive material to scientific community. Most studies on np-Au employ macro-scale specimens and focus on fundamental science of the material and its catalytic and sensor applications. The macro-scale specimens limit np-Au's potential in miniaturized systems, including biomedical devices. In order to address these issues, we initially describe two different methods to micropattern np-Au thin films on rigid substrates. The first method employs manually-produced stencil masks for creating millimeter-scale np-Au patterns, while the second method uses lift-off photolithography to pattern sub-millimeter-scale patterns. As the np-Au thin films are obtained by sputter-deposition process, they are compatible with conventional microfabrication techniques, thereby amenable to facile integration into microsystems. These systems include electrically-addressable biosensor platforms that benefit from high effective surface area, electrical conductivity, and gold-thiol-based surface bioconjugation. We describe cell culture, immunostaining, and image processing techniques to quantify np-Au's interaction with mammalian cells, which is an important performance parameter for some biosensors. We expect that the techniques illustrated here will assist the integration of np-Au in platforms at various length-scales and in numerous applications, including biosensors, energy storage systems, and catalysts. Video LinkThe video component of this article can be found at

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“…For example, NP Au-coated cantilevers undergo relevant chemo-mechanical deformation consequent to the adsorption of biomolecules [78]. NP Au-based sensors have shown remarkable selectivity towards specific prostate antigens [79] and probe DNA [80]. In addition, NP Au has been shown to allow effective electrochemical sensing of p-nitrophenol molecules [81], as well as the rapid measurement of Escherichia coli bacteria concentration in food and water [82].…”

Section: Np Au As the Typical Case Studymentioning

confidence: 99%

Mechanical Properties of Nanoporous Au: From Empirical Evidence to Phenomenological Modeling

Pia

Delogu

2015

Metals

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Abstract:The present work focuses on the development of a theoretical model aimed at relating the mechanical properties of nanoporous metals to the bending response of thick ligaments. The model describes the structure of nanoporous metal foams in terms of an idealized regular lattice of massive cubic nodes and thick ligaments with square cross-sections. Following a general introduction to the subject, model predictions are compared with Young's modulus and the yield strength of nanoporous Au foams determined experimentally and available in literature. It is shown that the model provides a quantitative description of the elastic and plastic deformation behavior of nanoporous metals, reproducing to a satisfactory extent the experimental Young's modulus and yield strength values of nanoporous Au.

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Detection of free prostate specific antigen (fPSA) on a nanoporous gold platform

Abstract: A new spectrophotometric method using covalently attached capture antibody labeled with alkaline phosphatase is developed for the detection of fPSA.

Cited by 59 publications

References 25 publications

Nanoporous Gold Electrodes and Their Applications in Analytical Chemistry

Nanoporous Gold Electrodes and Their Applications in Analytical Chemistry

Microfabrication of Nanoporous Gold Patterns for Cell-material Interaction Studies

Mechanical Properties of Nanoporous Au: From Empirical Evidence to Phenomenological Modeling

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