Detection of Fusobacterium necrophorum and Dichelobacter nodosus in lame cattle on dairy farms in New Zealand

Bennett, Grant; Hickford, Jonathan G. H.; Zhou, Huitong; LaPorte, Jeffrey A.; Gibbs, Jim

doi:10.1016/j.rvsc.2009.04.001

Cited by 27 publications

(14 citation statements)

References 11 publications

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“…Dichelobacter nodosus, which is described as the most common cause of lameness in sheep (Moore et al 2005), has been associated with cattle lameness for many years (Egerton and Parsonson 1966). Our survey's results are in agreement with the findings of previous researchers, who also reported detection of D. nodosus and F. necrophorum in similar proportions in cow footrot (Bennett et al 2009).…”

Section: Discussionsupporting

confidence: 92%

Detection of Fusobacterium necrophorum and Dichelobacter nodosus from cow footrot in the Heilongjiang Province, China

et al. 2019

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Cow footrot in the Heilongjiang Province, northeast China is a problem resulting in lost production in agriculture. In this study, 200 swab samples from footrot lesions of naturally infected cows with odorous exudative inflammation and keratinous hoof separation at 10 farms were examined in the period from May 2016 to May 2017. Twenty cows from each farm were taken for sampling. The samples were examined for detectingthe presence of Dichelobacter nodosus (D. nodosus) and Fusobacterium necrophorum (F. necrophorum). Such detection was carried out using polymerase chain reaction (PCR). The PCR primers were designed to identify the lktA gene, which encodes a leukotoxin unique to F. necrophorum, and the fimA gene of D. nodosus. Of the 200 samples, 111 (55.5%) revealed the presence of F. necrophorum and 11 (5.5%) exhibited D. nodosus. The frequent finding of F. necrophorum in cow farms of Heilongjiang province, northeast China is noteworthy. The possibility of F. necrophorum and D. nodosus infection should be an important concern when controlling cow footrot in China.

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Section: Discussionsupporting

confidence: 92%

Detection of Fusobacterium necrophorum and Dichelobacter nodosus from cow footrot in the Heilongjiang Province, China

et al. 2019

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“…In addition, Fusobacterium necrophorum , Treponema species and a range of other bacterial genera have been identified in the ovine interdigital skin (Roberts and Egerton 1969, Bennett and others 2009, Calvo‐Bado and others 2011, Frosth and others 2015). The role of F necrophorum in this disease still needs to be fully understood, with two hypothesis currently discussed: (i) F necrophorum is important to establish ID before D nodosus infection and hence initiates the disease (Egerton and others 1969) or (ii) F necrophorum is involved in the persistence and severity of footrot, once the under‐running lesion has developed, playing a role as an opportunistic, secondary pathogen (Witcomb and others 2014, 2015).…”

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confidence: 99%

Ovine footrot: new insights into bacterial colonisation

et al. 2016

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A note on versions:The version presented here may differ from the published version or from the version of record. If you wish to cite this item you are advised to consult the publisher's version. Please see the repository url above for details on accessing the published version and note that access may require a subscription. Ovine footrot is characterised by interdigital dermatitis (ID) and by the separation of the skin and hoof horn (underrunning footrot). Dichelobacter nodosus is the essential pathogen causing footrot; the role of other microorganisms in this disease remains unclear. The aims of this study were: (i) to investigate the colonisation of D. nodosus, Fusobacterium necrophorum and Treponema spp. in biopsies from the ovine interdigital skin of healthy, ID and footrot affected feet and (ii) to characterize the virulence of D. nodosus strains in those biopsies. Post-slaughter biopsy samples (n=241) were collected and analysed by real-time PCR to determine prevalence and load of the different bacterial species. The highest prevalence and load of D. nodosus were found on feet with ID. The vast majority of samples contained virulent D. nodosus and some samples contained both virulent and benign D. nodosus. Notably, the more pathogenic subspecies of F. necrophorum was found in samples from UK sheep. Our findings provide further insights into the role bacterial colonisation may play in the early stage of ID and in the progression towards footrot.https://mc.manuscriptcentral.com/vetrec Veterinary Record

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“…The association between D. nodosus and lameness in sheep is well known [ 16 ], but in cattle this association has not been completely elucidated. Bennett et al [ 17 ] considered it possible that the presence of the bacterium on the feet of lame cattle could be of significance for lameness. However, interdigital dermatitis, which is associated with D. nodosus , does normally not cause lameness in cattle [ 2 , 18 ], and the absence of lameness in the six heifers included in our study agrees with this previous finding and supports the hypothesis that the presence of D. nodosus in cattle is not necessarily associated with lameness.…”

Section: Resultsmentioning

confidence: 99%

Experimental infection of cattle with ovine Dichelobacter nodosus isolates

et al. 2015

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BackgroundDichelobacter nodosus is the main causative agent of ovine footrot, and there are strong indications that the bacterium can be transferred to cattle grazing on the same pasture as sheep. The aim of this study was to investigate if benign and virulent D. nodosus strains isolated from sheep can be transferred to the interdigital skin of cattle under experimental conditions. Further, we wanted to observe the impact of such infection on bovine foot health, and test the effect of topical chlortetracycline (Cyclo spray®: Eurovet) on the infection.FindingsSix heifers were included in the study. After an initial 18-day maceration period, three heifers were inoculated on one single foot with a benign strain and three with a virulent strain by adding bacterial suspension in a bandage. The bandages were left on for 17 days, and when removed, D. nodosus was isolated from all six heifers. All six heifers developed interdigital dermatitis. In five of the heifers D. nodosus organisms were demonstrated within the epidermis. Twenty-four days after treatment with chlortetracycline all heifers were negative by cultivation, but tested positive for D. nodosus by polymerase chain reaction (PCR). Two of the six heifers still tested positive for D. nodosus by PCR 49 days after treatment. After 70 days, all heifers tested negative for D. nodosus.ConclusionsThis study shows that both virulent and benign D. nodosus strains originating from sheep can be transferred to naïve heifers under experimental conditions. Further, the study supports the hypothesis that infections with virulent D. nodosus in cattle are associated with interdigital dermatitis. No conclusion regarding the treatment of D. nodosus infection with chlortetracycline was possible.

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Detection of Fusobacterium necrophorum and Dichelobacter nodosus in lame cattle on dairy farms in New Zealand

Cited by 27 publications

References 11 publications

Detection of Fusobacterium necrophorum and Dichelobacter nodosus from cow footrot in the Heilongjiang Province, China

Detection of Fusobacterium necrophorum and Dichelobacter nodosus from cow footrot in the Heilongjiang Province, China

Ovine footrot: new insights into bacterial colonisation

Experimental infection of cattle with ovine Dichelobacter nodosus isolates

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