Detection of Fusobacterium nucleatum in stool and colonic tissues from Norwegian colorectal cancer patients

Tunsjø, Hege Smith; Gundersen, Gro; Rangnes, Fredrik; Noone, J Christopher; Endres, Alexander; Bemanian, Vahid

doi:10.1007/s10096-019-03562-7

Cited by 42 publications

(34 citation statements)

References 36 publications

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“…These RQ studies reported a significantly higher F. nucleatum load in colorectal specimens of CRC patients compared to controls, except for two studies [50,52]. Fold change in F. nucleatum from controls to CRC cases was estimated by three studies with very different values: 132-fold according to Wong et al [56], 66-fold according to Tunsjo et al [61], and 5.2-fold reported by Xie et al [58].…”

Section: Comparison Of Fusobacterium Nucleatum Load In Colorectal Spementioning

confidence: 96%

“…In most of the included studies, feces were collected before colonoscopy or surgery, except for the study by Yu et al [60] where feces were collected more after colonoscopy than before. Tunsjo et al [61] also reported collecting feces either before colonoscopy or 1 week after. In four studies, no information was provided about the timing of specimen collection [43,54,57].…”

Section: Characteristics Of Studies Included In the Systematic Reviewmentioning

confidence: 99%

“…The cutoff values were typically set to the values that served to achieve the highest discrimination between CRC patients and controls in terms of Youden index. Tunsjo et al [61] set a cutoff value for detecting F. nucleatum in feces, but not in biopsies. As shown in Table 4, the cutoff value was not reported in one study [58] and varied between the three others: 260 copies of F. nucleatum by Suehiro et al [45] and a 2 −ΔCq of 0.00026 (2 −12 ) for both Eklof et al [47] and Tunsjo et al [61].…”

Section: Comparison Of Frequency Of Presence Of Fusobacterium Nucleatmentioning

confidence: 99%

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Detection of Fusobacterium nucleatum in feces and colorectal mucosa as a risk factor for colorectal cancer: a systematic review and meta-analysis

et al. 2020

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Background Colorectal cancer (CRC) is a major cause of cancer deaths worldwide. Accumulating evidence suggests a potentially important role of colorectal infection with Fusobacterium nucleatum (F. nucleatum) in colorectal carcinogenesis. We conducted a systematic review, including both a qualitative synthesis and a meta-analysis, to synthesize the evidence from the epidemiological literature on the association between F. nucleatum detection in the colon/rectum and CRC. Methods A systematic literature search of Ovid MEDLINE(R), Embase, Web of Science Core Collection, EBM Reviews—Cochrane Database of Systematic Reviews, and CINAHL Plus with Full Text was conducted using earliest inclusive dates up to 4 October 2020. Eligible studies were original, comparative observational studies that reported results on colorectal F. nucleatum detection and CRC. Two independent reviewers extracted the relevant information. Odds ratio (OR) estimates were pooled across studies using the random effects model. Newcastle-Ottawa scale was used to critically appraise study quality. Results Twenty-four studies were included in the systematic review, of which 12 were included in the meta-analysis. Studies investigated F. nucleatum in feces, colorectal tissue samples, or both. In most studies included in the systematic review, the load of F. nucleatum was higher, on average, in specimens from CRC patients than in those from CRC-free controls. Meta-analysis showed a positive association between F. nucleatum detection in colorectal specimens and CRC (OR = 8.3; 95% confidence interval (95% CI) 5.2 to 13.0). Conclusions The results of this systematic review suggest that F. nucleatum in the colon/rectum is associated with CRC. Systematic review registration This systematic review protocol has been registered with the International Prospective Register of Systematic Reviews (PROSPERO) on July 10, 2018 (registration number CRD42018095866).

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Section: Comparison Of Fusobacterium Nucleatum Load In Colorectal Spementioning

confidence: 96%

Section: Characteristics Of Studies Included In the Systematic Reviewmentioning

confidence: 99%

Section: Comparison Of Frequency Of Presence Of Fusobacterium Nucleatmentioning

confidence: 99%

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Detection of Fusobacterium nucleatum in feces and colorectal mucosa as a risk factor for colorectal cancer: a systematic review and meta-analysis

et al. 2020

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“…The formation of DNA adducts may account for the association between dietary NOC intake and rectal cancer (Loh et al, 2011;Zhu et al, 2014). Fusobacteria Fusobacterium -Rectal mucosa, colorectal tissue qPCR Significantly higher in CRA patients than in healthy controls, and in tumor tissue than in adjacent normal tissue (McCoy et al, 2013) Stool sample 16S rRNA pyrosequencing Significantly higher in CRC patients than in healthy controls (Wu et al, 2013) nucleatum Colorectal tissue, stool sample qPCR Significantly higher in CRA and CRC patients than in healthy controls (Kostic et al, 2013) Stool sample qPCR Significantly higher in CRC patients than in healthy controls or patients with polyps (Tunsjø et al, 2019) Stool sample qRT-PCR Significantly higher in CRA patients than in healthy controls (Fukugaiti et al, 2015) Colonic mucosa qPCR Significantly higher in tumor tissue than in adjacent normal tissue (Tahara et al, 2014;Viljoen et al, 2015) Colorectal tissue qPCR Higher abundance was associated with significantly higher CRC-specific mortality (Mima et al, 2016) Colorectal tissue FQ-PCR, FISH Significantly higher in tumor tissue than in adjacent normal tissue; overabundance was significantly associated with lymph node metastasis (Li et al, 2016) Firmicutes…”

Section: N-nitroso Compoundsmentioning

confidence: 99%

“…Indeed, many studies have revealed a consistent link between colorectal carcinogenesis and gut microbiota. Fusobacterium nucleatum (Kostic et al, 2013;Tahara et al, 2014;Fukugaiti et al, 2015;Viljoen et al, 2015;Li et al, 2016;Mima et al, 2016;Tunsjø et al, 2019), Streptococcus gallolyticus (Abdulamir et al, 2010;Butt et al, 2016;Corredoira et al, 2017;Kumar et al, 2017;Kwong et al, 2018), Clostridium difficile (Fukugaiti et al, 2015;Zheng et al, 2017), Clostridium septicum (Corredoira et al, 2017;Kwong et al, 2018), Enterococcus faecalis (Zhou et al, 2016;Rezasoltani et al, 2018;Geravand et al, 2019), Escherichia coli (Buc et al, 2013;Bonnet et al, 2014;Kohoutova et al, 2014;Dejea et al, 2018), Peptostreptococcus stomatis (Zeller et al, 2014;Yu et al, 2017), and Bacteroides fragilis (Boleij et al, 2015;Zhou et al, 2016;Purcell et al, 2017;Dejea et al, 2018;Kwong et al, 2018;Haghi et al, 2019) are differentially enriched in the fecal or colonic mucosa samples of CRC patients relative to healthy individuals, or in the CRC patient's tumor tissue relative to adjacent healthy tissue, wherein in some cases, CRC disease status is associated with the abundance of CRC-associated gut microbiota. CRC risk is also associated with the seroprevalence of Helicobacter pylori antibodies (Zhang et al, 2012;Epplein et al, 2013;Teimoorian et al, 2018;Butt et al, 2019;…”

Section: Introductionmentioning

confidence: 99%

Colon Carcinogenesis: The Interplay Between Diet and Gut Microbiota

Loke

Chew

Ngeow

et al. 2020

Front. Cell. Infect. Microbiol.

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Colorectal cancer (CRC) incidence increases yearly, and is three to four times higher in developed countries compared to developing countries. The well-known risk factors have been attributed to low physical activity, overweight, obesity, dietary consumption including excessive consumption of red processed meats, alcohol, and low dietary fiber content. There is growing evidence of the interplay between diet and gut microbiota in CRC carcinogenesis. Although there appears to be a direct causal role for gut microbes in the development of CRC in some animal models, the link between diet, gut microbes, and colonic carcinogenesis has been established largely as an association rather than as a cause-and-effect relationship. This is especially true for human studies. As essential dietary factors influence CRC risk, the role of proteins, carbohydrates, fat, and their end products are considered as part of the interplay between diet and gut microbiota. The underlying molecular mechanisms of colon carcinogenesis mediated by gut microbiota are also discussed. Human biological responses such as inflammation, oxidative stress, deoxyribonucleic acid (DNA) damage can all influence dysbiosis and consequently CRC carcinogenesis. Dysbiosis could add to CRC risk by shifting the effect of dietary components toward promoting a colonic neoplasm together with interacting with gut microbiota. It follows that dietary intervention and gut microbiota modulation may play a vital role in reducing CRC risk.

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Detection of colorectal‐cancer‐associated bacterial taxa in fecal samples using next‐generation sequencing and 19 newly established qPCR assays

Senthakumaran,

Tannæs,

Moen

et al. 2024

Molecular Oncology

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We have previously identified increased levels of distinct bacterial taxa within mucosal biopsies from colorectal cancer (CRC) patients. Following prior research, the aim of this study was to investigate the detection of the same CRC‐associated bacteria in fecal samples and to evaluate the suitability of fecal samples as a non‐invasive material for the detection of CRC‐associated bacteria. Next‐generation sequencing (NGS) of the 16S ribosomal RNA (rRNA) V4 region was performed to evaluate the detection of the CRC‐associated bacteria in the fecal microbiota of cancer patients, patients with adenomatous polyp and healthy controls. Furthermore, 19 novel species‐specific quantitative PCR (qPCR) assays were established to detect the CRC‐associated bacteria. Approximately, 75% of the bacterial taxa identified in biopsies were reflected in fecal samples. NGS failed to detect low‐abundance CRC‐associated taxa in fecal samples, whereas qPCR exhibited high sensitivity and specificity in identifying all targeted taxa. Comparison of fecal microbial composition between the different patient groups showed enrichment of Fusobacterium nucleatum, Parvimonas micra, and Gemella morbillorum in cancer patients. Our findings suggest that low‐abundance mucosa‐associated bacteria can be detected in fecal samples using sensitive qPCR assays.

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Detection of Fusobacterium nucleatum in stool and colonic tissues from Norwegian colorectal cancer patients

Cited by 42 publications

References 36 publications

Detection of Fusobacterium nucleatum in feces and colorectal mucosa as a risk factor for colorectal cancer: a systematic review and meta-analysis

Detection of Fusobacterium nucleatum in feces and colorectal mucosa as a risk factor for colorectal cancer: a systematic review and meta-analysis

Colon Carcinogenesis: The Interplay Between Diet and Gut Microbiota

Detection of colorectal‐cancer‐associated bacterial taxa in fecal samples using next‐generation sequencing and 19 newly established qPCR assays

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