Detection of galectin-3 in patients with inflammatory bowel diseases: new serum marker of active forms of IBD?

Frolova, L.; Smetana, Karel; Borovská, Dana; Kitanovičová, Andrea; Klimešová, Klára; Janatková, I; Malíčková, Karin; Lukáš, Milan; Drastich, Pavel; Beneš, Zdeněk; Tučková, Ludmila; Manning, Joachim C.; André, Sabine; Gabius, Hans‐Joachim; Tlaskalová‐Hogenová, Helena

doi:10.1007/s00011-009-0016-8

Cited by 38 publications

(40 citation statements)

References 48 publications

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“…There are reports where galectin-3 has been utilized as a marker to follow the inflammatory activity of a disease or used as a factor for prognosis in cancers or chronic heart failure [68]–[70]. Serum levels of galectin-3 have been used to monitor the inflammatory stage of inflammatory bowel disease [71]. Our finding that the expression of galectin-3 is increased in HP and SARC in BAL is in line with its role in inflammation.…”

Section: Discussionsupporting

confidence: 78%

Proteomic Changes of Alveolar Lining Fluid in Illnesses Associated with Exposure to Inhaled Non-Infectious Microbial Particles

et al. 2014

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BackgroundHyperresponsiveness to inhaled non-infectious microbial particles (NIMPs) has been associated with illnesses in the airways. Hypersensitivity pneumonitis (HP) is considered to be the prototype for these NIMPs-related diseases; however, there is no consensus on the definitions or diagnostic criteria for HP and the spectrum of related illnesses.Methods and FindingsIn order to identify the possible diagnostic markers for illnesses associated with NIMPs in alveolar lining fluid, we performed a proteomic analysis using a two-dimensional difference gel electrophoresis on bronchoalveolar lavage (BAL) fluid from patients with exposure to NIMPs in the context of damp building-related illness (DBRI) or conditions on the borderline to acute HP, designated here as agricultural type of microbial exposure (AME). Samples from patients with HP and sarcoidosis (SARC) were included for reference. Results were compared to results of healthy subjects (CTR). Western blot was used for validation of potential marker proteins from BAL fluid and plasma. Protein expression patterns suggest a close similarity between AME and HP, while DBRI was similar to CTR. However, in DBRI the levels of the inflammation associated molecules galectin-3 and alpha-1-antitrypsin were increased. A novel finding emerging from this study was the increases of semenogelin levels in BAL fluid from patients with AME, HP and SARC. Histone 4 levels were increased in AME, HP and SARC. Elevated plasma levels of histone 2B were detected in HP and SARC, suggesting it to be a potential blood indicator for inflammatory diseases of the lungs.ConclusionsIn this study, the proteomic changes in bronchoalveolar lavage of DBRI patients were distinct from other NIMP exposure associated lung diseases, while changes in AME overlapped those observed for HP patient samples. Some of the proteins identified in this study, semenogelin and histone 4, could function as diagnostic markers for differential diagnosis between DBRI and HP-like conditions.

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Section: Discussionsupporting

confidence: 78%

Proteomic Changes of Alveolar Lining Fluid in Illnesses Associated with Exposure to Inhaled Non-Infectious Microbial Particles

et al. 2014

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“…Galectin-3 levels in healthy adult plasma average at 12 ng/ml [15,21] and increases 3 to 5-fold as a result of different pathologies and inflammatory processes [14-17]. No reports have so far been published on the normal galectin-3 levels in neonatal plasma, why we evaluated the amount in cord blood plasma after CS.…”

Section: Resultsmentioning

confidence: 99%

“…Galectin-3 has emerged as an important marker in several settings of inflammation [14-17]. In the context of perinatal biology, we have found that galectin-3 contributes to brain injury in a murine model of hypoxic ischemia [18] and is found in increased amounts in asphyxiated infants with abnormal outcome [19].…”

Section: Introductionmentioning

confidence: 99%

Cord blood neutrophils display a galectin-3 responsive phenotype accentuated by vaginal delivery

et al. 2013

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BackgroundTerm neonates are at increased risk of infections due to undeveloped immune mechanisms, and proper neutrophil function is important for perinatal immune defence. Galectin-3, an endogenous β-galactoside-binding lectin, is emerging as an inflammatory mediator and we have previously shown that primed/activated, but not resting, adult neutrophils respond to this lectin by production of reactive oxygen species (ROS). We investigated if galectin-3 is of importance in perinatal immune defence, focusing on plasma levels and neutrophil responsiveness.MethodsNeutrophils were isolated from peripheral blood of healthy adults and cord blood (CB) after elective Caesarean section (CSCB) and vaginal delivery (VDCB). ROS production was measured by chemiluminescence, L-selectin expression by flow cytometry, and interleukin-8 (IL-8) and galectin-3 concentrations by ELISA. Statistical evaluations were performed using the Mann–Whitney test.ResultsIn response to galectin-3, CSCB neutrophils showed a small but clear ROS production not evident in adult cells, signifying that neonatal neutrophils exist in a primed state. IL-8 production was elevated in CSCB cells while L-selectin exposure was equal to adult cells. Comparing CSCB to VDCB neutrophils, the latter showed an extensive galectin-3 responsiveness, indicating that the degree of priming is dependent on mode of delivery. VDCB neutrophils were increasingly prone to shed L-selectin, while the amount of IL-8 was similar to CSCB cells. The endogenous galectin-3 levels were higher in neonatal as compared to adult plasma, unaffected by mode of delivery.ConclusionsNeutrophils enter a pre-primed state already in the fetus. Upon exposure to the inflammatory stimuli that are associated with labor, the neutrophils develop a reactive phenotype with extensive priming features.

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“…It plays an important disease-exacerbating role in autoimmune/inflammatory and malignant diseases (5-9) but has a protective role in obesity-induced inflammation and type 2 diabetes, as well as in primary biliary cirrhosis (10,11). Recently, several clinical studies showed a correlation between the serum level of Gal-3 and exacerbation of acute colitis, indicating the importance of Gal-3 as a potential marker for this disease (12)(13)(14). Serum concentrations of Gal-3 were significantly increased in specimens from patients with the active form of UC (12).…”

Section: Introductionmentioning

confidence: 99%

“…Recently, several clinical studies showed a correlation between the serum level of Gal-3 and exacerbation of acute colitis, indicating the importance of Gal-3 as a potential marker for this disease (12)(13)(14). Serum concentrations of Gal-3 were significantly increased in specimens from patients with the active form of UC (12). Recently, we showed that Gal-3 plays an important proinflammatory role in the induction phase of acute DSSinduced colitis by promoting activation of the NLRP3 inflammasome and production of IL-1β in macrophages (7).…”

Section: Introductionmentioning

confidence: 99%

Bacterial Flora Play Important Roles in Acute Dextran Sulphate Sodium-Induced Colitis But Are Not Involved in Gal-3 Dependent Modulation of Colon Inflammation

Marković

Milosavljevic

Arsenijević

et al. 2017

Serbian Journal of Experimental and Clinical Research

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Detection of galectin-3 in patients with inflammatory bowel diseases: new serum marker of active forms of IBD?

Abstract: Enhanced concentration of galectin-3 in serum reflects presence of disease and points to its involvement in the pathogenesis.

Cited by 38 publications

References 48 publications

Proteomic Changes of Alveolar Lining Fluid in Illnesses Associated with Exposure to Inhaled Non-Infectious Microbial Particles

Proteomic Changes of Alveolar Lining Fluid in Illnesses Associated with Exposure to Inhaled Non-Infectious Microbial Particles

Cord blood neutrophils display a galectin-3 responsive phenotype accentuated by vaginal delivery

Bacterial Flora Play Important Roles in Acute Dextran Sulphate Sodium-Induced Colitis But Are Not Involved in Gal-3 Dependent Modulation of Colon Inflammation

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