Detection of Gene Flow from Sexual to Asexual Lineages in Thrips tabaci (Thysanoptera: Thripidae)

Li, Xiaowei; Wang, Ping; Fail, József; Shelton, Anthony M.

doi:10.1371/journal.pone.0138353

Cited by 19 publications

(34 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…tabaci as a cryptic species complex even though ongoing gene flow occurs. Results from this study and Li et al 2015 [ 15 ] show that gene-flow events are rare; however, it is not clear whether or not offspring produced from these events persist in the environment. Future studies examining both gene flow among different clonal groups of T .…”

Section: Discussioncontrasting

confidence: 65%

“…Phylogenetic relationships examined using ML methods in MEGA identified two major clades in this study, which corresponded to lineages 2 and 3 ( Fig 2 ) [ 1 , 6 , 7 , 9 , 17 ]. Most haplotypes collected in this study were previously reported in New York: lineage 3 haplotypes NY-HT1, NY-HT2/NY-HT5 (these two could not be distinguished based on the sequences used in this analysis), NY-HT3, NY-HT6 [ 17 ], and lineage 2 haplotype, here designated as NY-HA1 [ 15 ]. Two new haplotypes in lineage 2, NY-HA2 (Genbank: KR152257), and NY-HA3 (Genbank: KR152258) were identified in this study.…”

Section: Resultsmentioning

confidence: 99%

“…Another recently published study conducted in New York, USA also challenges the strict association of T . tabaci lineages with reproductive mode [ 15 ]. In this study males from lineage 2 readily mated with females from lineage 3 in the laboratory, and examination of offspring from these matings showed that genetic exchange between parents occurred in 4 out of 75 offspring produced from mated females.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Restricted Gene Flow among Lineages of Thrips tabaci Supports Genetic Divergence Among Cryptic Species Groups

et al. 2016

View full text Add to dashboard Cite

Knowledge of the relative influence of population- versus species-level genetic variation is important to understand patterns of phenotypic variation and ecological relationships that exist among and within morphologically indistinguishable cryptic species and subspecies. In the case of cryptic species groups that are pests, such knowledge is also essential for devising effective population management strategies. The globally important crop pest Thrips tabaci is a taxonomically difficult group of putatively cryptic species. This study examines population genetic structure of T. tabaci and reproductive isolation among lineages of this species complex using microsatellite markers and mitochondrial COI sequences. Overall, genetic structure supports T. tabaci as a cryptic species complex, although limited interbreeding occurs between different clonal groups from the same lineage as well as between individuals from different lineages. These results also provide evidence that thelytoky and arrhenotoky are not fixed phenotypes among members of different T. tabaci lineages that have been generally associated with either reproductive mode. Possible biological and ecological factors contributing to these observations are discussed.

show abstract

Section: Discussioncontrasting

confidence: 65%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Restricted Gene Flow among Lineages of Thrips tabaci Supports Genetic Divergence Among Cryptic Species Groups

et al. 2016

View full text Add to dashboard Cite

show abstract

“…It is worth pointing out that in its current form, our method is not able to detect incidental gene flow between thelytokous and arrhenotokous individuals. Li et al (2015) published evidence about interbreeding by detecting gene flow between the progeny of leek-associated arrhenotokous (L1) males and leek-associated thelytokous (L2) females. Due to the successful mating between different lineages, the male-originated nuclear gene allele (histone H3 gene) was present in the progenies, although in only very low proportions.…”

Section: Discussionmentioning

confidence: 99%

A simple molecular identification method of theThrips tabaci(Thysanoptera: Thripidae) cryptic species complex

Farkas

György

Tóth

et al. 2019

Bull. Entomol. Res.

View full text Add to dashboard Cite

The onion thrips (Thrips tabaci Lindeman, 1889) is a key pest of a wide range of crops because of its ecological attributes such as polyphagy, high reproduction rate, ability to transmit tospoviruses and resistance to insecticides. Recent studies revealed that T. tabaci is a cryptic species complex and it has three lineages (leek-associated arrhenotokous L1-biotype, leek-associated thelytokous L2-biotype and tobacco-associated arrhenotokous T-biotype), however, the adults remain indistinguishable. T. tabaci individuals were collected from different locations of Hungary to create laboratory colonies from each biotypes. Mitochondrial COI (mtCOI) region was sequenced from morphologically identified individuals. After sequence analysis SNPs were identified and used for CAPS marker development, which were suitable for distinguishing the three T. tabaci lineages. Genetic analysis of the T. tabaci species complex based on mtCOI gene confirmed the three well-known biotypes (L1, L2, T) and a new biotype because the new molecular evidence presented in this study suggests T-biotype of T. tabaci forming two distinct (sub)clades (T1 and T2). This genetic finding indicates that the genetic variability of T. tabaci populations is still not fully mapped. We validated our developed marker on thrips individuals from our thrips colonies. The results demonstrated that the new marker effectively identifies the different T. tabaci biotypes. We believe that our reliable genotyping method will be useful in further studies focusing on T. tabaci biotypes and in pest management by scanning the composition of sympatric T. tabaci populations.

show abstract

“…The parasite's life cycle thus likely represents a continuum of bottlenecks linked to frequent local extinction and recolonization events that increase levels of genetic drift and identity by descent (IBD). It may thus come to less surprise that observations of diffuse hybrid clonality around a restricted focus of sex in BM resemble spatio-temporal patterns of heterogony demonstrated in various other metapopulation systems (50)(51)(52)(53)(54)(55)(56)(57)(58). Facultative sex often coincides with strong metapopulation structure, in which sexual variants are predicted to occupy core habitat (where population subdivision and inbreeding depression is minimized) while asexual variants disperse more freely without fitness costs from close IBD during frequent founding events (59).…”

Section: Rationale For Reproductive Polymorphism In T Cruzimentioning

confidence: 99%

Parallel sexual and parasexual population genomic structure inTrypanosoma cruzi

Schwabl

Imamura

Broeck

et al. 2018

Preprint

View full text Add to dashboard Cite

Genetic exchange and hybridization in parasitic organisms is fundamental to the exploitation of new hosts and host populations. Variable mating frequency often coincides with strong metapopulation structure, where patchy selection or demography may favor different reproductive modes. Evidence for genetic exchange in Trypanosoma cruzi over the last 30 years has been limited and inconclusive. The reproductive modes of other medically important trypanosomatids are better established, although little is known about their variability on a spatio-temporal scale. Targeting a contemporary focus of T. cruzi transmission in southern Ecuador, we present compelling evidence from 45 sequenced genomes that T. cruzi (discrete typing unit I) maintains sexual populations alongside others that represent clonal bursts of parasexual origin. Strains from one site exhibit genome-wide Hardy-Weinberg equilibrium and intra-chromosomal linkage decay consistent with meiotic reproduction. Strains collected from adjacent areas (>6 km) show excess heterozygosity, near-identical haplo-segments, common mitochondrial sequences and levels of aneuploidy incompatible with Mendelian sex. Certain individuals exhibit trisomy in as many as fifteen chromosomes. Others present fewer, yet shared, aneuploidies reminiscent of mitotic genome erosion and parasexual genetic exchange. Genomic and intra-genomic phylogenetics as well as haplotype co-ancestry analyses indicate a clear break in geneflow between these distinct populations, despite the fact that they occasionally co-occur in vectors and hosts. We propose biological explanations for the fine-scale disconnectivity we observe and discuss the epidemiological consequences of flexible reproductive modes and their genomic architecture for this medically important parasite.

show abstract

Detection of Gene Flow from Sexual to Asexual Lineages in Thrips tabaci (Thysanoptera: Thripidae)

Cited by 19 publications

References 51 publications

Restricted Gene Flow among Lineages of Thrips tabaci Supports Genetic Divergence Among Cryptic Species Groups

Restricted Gene Flow among Lineages of Thrips tabaci Supports Genetic Divergence Among Cryptic Species Groups

A simple molecular identification method of theThrips tabaci(Thysanoptera: Thripidae) cryptic species complex

Parallel sexual and parasexual population genomic structure inTrypanosoma cruzi

Contact Info

Product

Resources

About