Detection of genes for enterotoxins (ent) and toxic shock syndrome toxin-1 (tst) in mammary isolates of Staphylococcus aureus by polymerase-chain-reaction

Zschöck, M.; Botzler, D.; Blöcher, Sonja; Sommerhäuser, Jürgen; Hamann, Hans-Peter

doi:10.1016/s0958-6946(00)00084-4

Cited by 42 publications

(26 citation statements)

References 32 publications

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“…Regarding the frequency of each gene investigated, the sec gene was the most prevalent, occurring in 15 strains (44.1%) of S. aureus, which was concordant with data from a study carried out in Germany, where 34 strains of S. aureus isolated from different dairy farms that amplified any gene (sea-see and tst) found that the sec gene occurred most frequently in 22 of the strains (64.7%), followed by the tst gene in 19 (55.8%) (Zschöck et al, 2000).…”

Section: Pereira Et Al 527supporting

confidence: 88%

Toxigenic Staphylococcus aureus in processing of coalho and mozzarella cheese

Pereira¹,

Machado²,

Lopes³

et al. 2017

Afr. J. Microbiol. Res.

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Staphylococcus aureus is commonly involved in food poisoning due to production of toxins responsible for causing animal and human diseases. In this study, 60 strains of presumptive S. aureus isolates from raw milk and cheese were biochemically identified in four dairies: 54 (90%) from refrigerated raw milk (RRM) with counts exceeding 10 6 CFU/mL, and six (10%) from cheese with similar concentrations of CFU/mL. Out of the 60 strains of presumptive S. aureus, 46 (76.7%) amplified the femA gene and then they were investigated regarding the presence of the Toxic Shock Syndrome Toxin-1 (TSST-1) gene and the classical enterotoxin genes (SEs) types A, B, C, D and E: 31 (67.4%) carried one or more encoding toxin genes, and 13 different genotypes were identified. Twenty-one strains (61.8%) carried one gene; three (8.8%), two genes; seven (20.6%), three genes; two (5.8%), four genes; and one (3%), five genes. The sec gene was the most frequent one, followed by seb and tst. The sed gene was expressed by 10 strains (29.4%), sea by five (14.7%) and see by three (8.8%). The S. aureus isolates showed genetic potential for producing toxins of importance for public health that presented a danger of food poisoning.

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Section: Pereira Et Al 527supporting

confidence: 88%

Toxigenic Staphylococcus aureus in processing of coalho and mozzarella cheese

Pereira¹,

Machado²,

Lopes³

et al. 2017

Afr. J. Microbiol. Res.

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“…Some researchers (Zschöck et al, 2000;Chen et al, 2001;Mason et al, 2001) used lysostaphin as the lytic agent during extraction, and had acceptable results for PCR. Johnson and Stell (2000) indicated that for several bacteria, including some Gram-positive, DNA of sufficient quantity and quality for diagnostic PCR can be directly obtained by boiling the organisms in water, followed by centrifugation to remove bacterial debris.…”

Section: Discussionmentioning

confidence: 99%

“…But Zschöck et al (2000) described the detection of all types of staphylococcal toxin genes (SEA-SEE and TSST-1) using nanogram quantities of target DNA in a 50µl PCR reaction. In this paper, the quantity and quality of DNA obtained using both methods were sufficiently good to amplify the expected DNA fragments, although the use of lysostaphin resulted in overall better DNA yields.…”

Section: Discussionmentioning

confidence: 99%

An alternative method for Staphylococcus aureus DNA isolation

Chapaval

Moon

Gomes

et al. 2008

Arq. Bras. Med. Vet. Zootec.

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This study describes a rapid procedure for the isolation of genomic DNA from Staphylococcus aureus that yielded a good amount of high quality DNA for the amplification of staphylococcal enterotoxins genes (A, B, C, D, and E) and the TSST-1 gene as well as enzymatic restriction (HaeIII) from environmental isolates. With this method, it was possible to detect these genes in a sample containing as little as 10 5

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“…Of these bacteria, Staphylococcus aureus has frequently been isolated from bovine mastitis (Watts, 1988) and it can directly be transferred by infected milk and/or dairy products or indirectly through environmental contamination of milk during handling and processing (Jorgensen et al, 2005). Protein toxins and other virulence factors produced by S. aureus strains isolated from subclinical and clinical mastitis cases are thought to contribute to the pathogenicity of the organism (Zschokck, 2000). TSST-1 is one of virulence factors which consists of a single-chain polypeptide with a molecular weight of about 22 KDa and an isoelectric point of 7.2.…”

Section: Introductionmentioning

confidence: 99%

Detection of toxic shock toxin (tst) gene in Staphylococcus aureus isolated from bovine milk samples

Baniardalan¹,

Mohammadzadeh²,

Pajohi‐Alamoti³

et al. 2017

BJVM

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Staphylococcus aureus is a major causative pathogen of clinical and subclinical mastitis in dairy cattle all over the world. This agent produces a variety of extracellular toxins and virulence factors including toxic shock syndrome toxin-1 (TSST-1) which is the major cause of toxic shock syndrome (TSS). In the present study, 76 S. aureus isolates have been obtained from milk samples collected from 7 dairy herds in Hamedan province of Iran. The isolates were identified based on the biochemical and molecular methods using PCR amplification of the femA gene. The staphylococcal isolates were also examined for the presence of TSST-1 (tst) encoding gene. This gene was detected in only one S. aureus isolate (1.3%). The results revealed that S. aureus strains causing bovine mastitis may potentially produce staphylococcal toxic shock syndrome toxin-1, indicating that it is very important to follow the presence of TSST-1 producing S. aureus isolates in foodstuffs to protect consumers against the risk of toxic shock syndrome.

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Detection of genes for enterotoxins (ent) and toxic shock syndrome toxin-1 (tst) in mammary isolates of Staphylococcus aureus by polymerase-chain-reaction

Cited by 42 publications

References 32 publications

Toxigenic Staphylococcus aureus in processing of coalho and mozzarella cheese

Toxigenic Staphylococcus aureus in processing of coalho and mozzarella cheese

An alternative method for Staphylococcus aureus DNA isolation

Detection of toxic shock toxin (tst) gene in Staphylococcus aureus isolated from bovine milk samples

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