Detection of Genetic Polymorphism in Seven Barley Hordeum vulgare L. Varieties Using SSR

Abstract

Investigation was carried out at the laboratory of Biotechnology, Faculty of Agriculture, Damascus University, during the season 2017-2018. Seven varieties were planted to determine the degree of genetic similarity using SSR-technique (Simple Sequence Repeats), and 14 double primers were used for this purpose. The analysis results revealed that all primers showed polymorphism among the evaluated varieties, except Bmag0385. primers produced a total of 42 alleles with a polymorphic percentage of 88.27%. The number of alleles for each primer varied from 1 allele for the primer (Bmac0067) to 7 alleles for the primer (Bmag0006) in average of 3 alleles per primer. Cluster analysis and Dendrogram showed the highest degree of genetic similarity between variety Arabi asuad and variety Arabi abiad (0.7619). While it was low between variety Fourat4 and variety Arabi abiad (0.3571), and varieties Fourat4 and Fourat3 (0.3571) which indicated wide genetic diversity among them.

Effec of Salt Stress on Yield and Its Components of Four Cultivars of Barler

“…The upper parts of the plant, causes a weakening of vital processes, especially photosynthesis, and a decrease in the percentage of chlorophyll in the green parts, in addition to accelerating their aging (19). Also, the high concentration of potassium in the upper parts at the expense of sodium leads to an increase in the value of the ratio of potassium to sodium, and this is one of the important and effective issues in salinity tolerance mechanisms ( 11,14,15).…”

“…The experimental land was plowed, prepared and divided into plots according to the treatments to represent the experimental units. The experiment comprised three degrees of irrigation water salinity, which are S1, S2, and S3 (5,10,15) ds.m -1 , respectively, and four cultivars of barley, namely (IPA 99, Buhooth 265, Samir and Amal). Randomized Complete Block Design with, three replicates was used, Planting was completed on November 26th for both seasons in plots (6 m 2 ) on lines with a 20 cm spacing between lines, using a seeding rate of 120 kg.…”