Detection of genetically modified plant products by protein strip testing: an evaluation of real-life samples

Bulcke, Marc Van den; Schrijver, Adinda De; Bernardi, Daniele; Devos, Yann; MbongoMbella, Guillaume; Casi, Amaya Leunda; Moens, William; Sneyers, Myriam

doi:10.1007/s00217-006-0381-2

Cited by 34 publications

(8 citation statements)

References 12 publications

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“…Two additional qPCR applications (SIMQUANT; Berdal et al 2008) use qPCR chemistry together with the limiting dilutions principle, which is near to the idea of the ddPCR-based methods, of which two were included Dobnik et al 2015). Other selected methods include LAMP with end-point fluorescent (Chen et al 2011;Wang et al 2015) or bioluminescence real-time detection (Kiddle et al 2012), multiplex PCR with hybridization on microarrays (Leimanis et al 2008;Hamels et al 2009) or detection with capillary gel electrophoresis (Nadal et al 2006), a protein-based method (Van Den Bulcke et al 2007), and two NGS methods (unpublished, developed within the EU FP7 Decathlon project), one for enriched samples and another for whole genome sequencing (see, e.g., Arulandhu et al 2016;Holst-Jensen et al 2016). The majority of the selected methods are validated in-house or within international collaborative trials and their fitness for purpose demonstrated elsewhere (see Table 1 for references).…”

Section: Analytical Methods Assessed In This Studymentioning

confidence: 99%

Decision Support for the Comparative Evaluation and Selection of Analytical Methods: Detection of Genetically Modified Organisms as an Example

et al. 2018

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The selection of the best-fit-for-purpose analytical method to be implemented in the laboratory is difficult due to availability of multiple methods, targets, aims of detection, and different kinds and sources of more or less reliable information. Several factors, such as method performance, practicability, cost of setup, and running costs need to be considered together with personnel training when selecting the most appropriate method. The aim of our work was to prepare a flexible multicriteria decision analysis model suitable for evaluation and comparison of analytical methods used for the purpose of detecting and/or quantifying genetically modified organisms, and to use this model to evaluate a variety of changing analytical methods. Our study included selection of PCR-, isothermal-, protein-, microarray-, and next-generation sequencing-based methods in simplex and/or multiplex formats. We show that the overall result of their fitness for purpose is relatively similar; however, individual criteria or a group of related criteria exposed more substantial differences between the methods. The proposed model of this decision support system enables easy modifications and is thus suitable for any other application of complex analytical methods.

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Section: Analytical Methods Assessed In This Studymentioning

confidence: 99%

Decision Support for the Comparative Evaluation and Selection of Analytical Methods: Detection of Genetically Modified Organisms as an Example

et al. 2018

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“…Protein strip tests represent a useful tool to trace proteins in raw materials such as seeds and leaves from crop plants. In food/feed products, the protein strip test applicability in GM tracing is restricted to samples containing suffi cient GM material derived from plant tissues where the recombinant protein is expressed and limited by the inherent physicochemical properties of the proteins themselves (thermostability, quenching interference; Van den Bulcke et al 2007 ) .…”

Section: Dna-based and Immunological Methodsmentioning

confidence: 99%

How to Reliably Test for GMOs

Žel

Milavec

Morisset

et al. 2011

How to Reliably Test for GMOs

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Fig. 1 Comparison of GM insect resistant and non-GM maize. The recombinant DNA sequence present in GM maize, target for the DNA-based detection of GM, is enlarged How to Reliably Test for GMOsTable 1 List of websites useful for detection of GMOs Short description Website Sources of Information on GMOs The Biosafety Clearing-House (BCH) is a mechanism setup by the Cartagena Protocol on Biosafety to facilitate the exchange of information on Living Modifi ed Organisms (LMOs) and assist the parties to better comply with their obligations under the Protocol. It provides global access to a variety of scientifi c, technical, environmental, legal, and capacity-building information and links to National BCH. http://bch.cbd.int/ BCH also includes LMO Registry. http://bch.cbd.int/database/organisms/ uniqueidentifi ers/ Centre for Environmental Risk Assessment (CERA) 's database of safety information (formerly hosted by AGBIOS) includes not only plants produced using recombinant DNA technologies, but also plants with novel traits that may have been produced using more traditional methods, such as accelerated mutagenesis or plant breeding. http://www.cera-gmc.org/?action =gm_crop_database OECD Biosafety including BioTrack Product Database. http://www2.oecd.org/biotech/ International Service for the Acquisition of Agri-biotech Applications , publishes Global Status of Commercialized Biotech Crops annually. http://www.isaaa.org/ United States Regulatory Agencies Unifi ed Biotechnology Website. http://usbiotechreg.nbii.gov/ Information Systems for Biotechnology (ISB) provide information resources to support the environmentally responsible use of agricultural biotechnology products. They include documents and searchable databases pertaining to the development, testing, and regulatory review of genetically engineered plants, animals, and microorganisms within the United States and abroad (supported by USDA). http://www.nbiap.vt.edu/ Biotechnology Industry Organization http://www.bio.org/ European Union (EU) GM food and feed, legislation, authorization, labeling, notifi cation of existing products. http://ec.europa.eu/food/food/ biotechnology/ gmfood/index_en.htm European Food Safety Authority (EFSA) , GMO panel. http://www.efsa.europa.eu/en/panels/ gmo.htm European Union Reference Laboratory for GM Food and Feed (EU-RL GMFF). http://gmo-crl.jrc.ec.europa.eu/ GMO register : deliberate release and placing GMOs on the EU market. http://gmoinfo.jrc.ec.europa.eu/ GMO compass is the work of independent science journalists, including the status of GMO applications/ approvals in the European Union, and other news from plant biotechnology, presented to the public in a way that is easy to understand and readily accessible.

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“…between tissues and varieties (Grothaus et al, 2006;SeedQuest, 2011;Van den Bulcke et al, 2007). In some cultivars, the protein content may be even lower than the quantification limit.…”

Section: Other Gmo Detection Targetsmentioning

confidence: 99%

Advances in identifying GM plants: current frame of the detection of transgenic GMOs

Bertheau¹

2021

Burleigh Dodds Series in Agricultural Science

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Transgenic GMOs were welcomed in the 1990s due to the difficulties distinguishing genetic and epigenetic modifications from random mutagenesis and their ability to insert new nucleic sequences more rapidly but still randomly. Their marketing in Europe has been accompanied by health and environmental risk assessments, specific monitoring and traceability procedures to preserve the free choice of consumers and allow the coexistence of different supply chains. This chapter reviews the regulations, detection techniques, strategies and standards that have been put in place in the European Union since 1996 to ensure the analytical traceability of these GMOs. The capacity of the matrix approach, initially targeted at transgenic GMOs, to trace other types of GMOs is discussed in an accompanying chapter.

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Detection of genetically modified plant products by protein strip testing: an evaluation of real-life samples

Cited by 34 publications

References 12 publications

Decision Support for the Comparative Evaluation and Selection of Analytical Methods: Detection of Genetically Modified Organisms as an Example

Decision Support for the Comparative Evaluation and Selection of Analytical Methods: Detection of Genetically Modified Organisms as an Example

How to Reliably Test for GMOs

Advances in identifying GM plants: current frame of the detection of transgenic GMOs

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