Detection of Goose and Muscovy Duck Parvoviruses using Polymerase Chain Reaction-Restriction Enzyme Fragment Length Polymorphism Analysis

Sirivan, Phontip; Obayashi, Makiko; Nakamura, Masayuki; Tantaswasdi, Urasri; Takehara, Kazuhiko

doi:10.2307/1592585

Cited by 28 publications

(20 citation statements)

References 10 publications

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“…All PCR reactions were carried out using the PCR machine (Biometra, Goettingen, Germany). aMPV-C was detected by RT-PCR as described by Ali and Reynolds [18]; BYD virus was detected by RT-PCR as described by Yan et al [19] and Su et al [2]; avian influenza virus was detected by RT-PCR as described by Fereidouni et al [20]; Newcastle disease virus was detected by RT-PCR as described by Gohm et al [21]; duck reovirus was detected by PCR as described by Zhang et al [22]; goose parvovirus was detected by PCR as described by Limn et al [23]; Egg drop syndrome-76 virus was detected by PCR as described by Kumar et al [24]; duck parvovirus was detected by PCR as described by Sirivan et al [25].…”

Section: Viral Rna Extraction Pcr and Sequence Analysismentioning

confidence: 99%

Isolation and characterization of a subtype C avian metapneumovirus circulating in Muscovy ducks in China

Sun

Chen

Cao

et al. 2014

Vet Res

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Subtype C avian metapneumovirus (aMPV-C), is an important pathogen that can cause egg-drop and acute respiratory diseases in poultry. To date, aMPV-C infection has not been documented in Muscovy ducks in China. Here, we isolated and characterized an aMPV-C, designated S-01, which has caused severe respiratory disease and noticeable egg drop in Muscovy duck flocks in south China since 2010. Electron microscopy showed that the isolate was an enveloped virus exhibiting multiple morphologies with a diameter of 20-500 nm. The S-01 strain was able to produce a typical cytopathic effect (CPE) on Vero cells and cause death in 10-to 11-day-old Muscovy duck embryos. In vivo infection of layer Muscovy ducks with the isolate resulted in typical clinical signs and pathological lesions similar to those seen in the original infected cases. We report the first complete genomic sequence of aMPV-C from Muscovy ducks. A phylogenetic analysis strongly suggested that the S-01 virus belongs to the aMPV-C family, sharing 92.3%-94.3% of nucleotide identity with that of aMPV-C, and was most closely related to the aMPV-C strains isolated from Muscovy ducks in France. The deduced eight main proteins (N, P, M, F, M2, SH, G and L) of the novel isolate shared higher identity with hMPV than with other aMPV (subtypes A, B and D). S-01 could bind a monoclonal antibody against the F protein of hMPV. Together, our results indicate that subtype-C aMPV has been circulating in Muscovy duck flocks in South China, and it is urgent for companies to develop new vaccines to control the spread of the virus in China.

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Section: Viral Rna Extraction Pcr and Sequence Analysismentioning

confidence: 99%

Isolation and characterization of a subtype C avian metapneumovirus circulating in Muscovy ducks in China

Sun

Chen

Cao

et al. 2014

Vet Res

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“…The standard phenol Á/chloroform method was used for DNA isolation. PCR was carried out with the primers and conditions described for the amplification of GPV (Sirivan et al ., 1998), GHPV (Guerin et al ., 1999) and GCV (Ball et al ., 2004). The products were analysed on 2% agarose gel and visualized under ultraviolet light after staining with 0.1% ethidium bromide.…”

Section: Methodsmentioning

confidence: 99%

Pathology of goose haemorrhagic polyomavirus infection in goose embryos

et al. 2006

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Goose embryos were infected with goose haemorrhagic polyomavirus (GHPV) onto the chorioallantoic membrane (CAM) in order to examine the effect of GHPV on the embryos and to obtain data on whether embryos could develop into infected, virus-shedding goslings, as well as to present an accurate biological method for virus titration. The reported method of infection could offer a possibility to express the virus titre as the median embryo infective dose (EID 50 ). As a special pathological feature of the disease, extensive cerebral haemorrhages were observed, which protruded the skullcap in many cases. Some embryos infected with 101.25 or 10 0.25 EID 50 /0.2 ml were able to hatch; however, they were in poor physical condition and died by post-hatching day 4 showing haemorrhagic nephritis and enteritis of geese. Virus shedding was revealed by polymerase chain reaction. The ability of some of the infected goose embryos to hatch may indicate the potency of GHPV to spread vertically, although this needs further study for confirmation.

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“…In order to screen for GPV PCR using primers and reaction conditions published by Sirivan et al (1998) was used.…”

Section: Pcrmentioning

confidence: 99%

Case report of goose haemorrhagic polyomavirus in 4-day-old goslings indicating vertical transmissibility

2011

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Haemorrhagic nephritis and enteritis of geese (HNEG) is a fatal disease caused by goose haemorrhagic polyomavirus (GHPV). The aim of our study was to investigate a field outbreak of HNEG by pathological methods and real-time PCR assay using light upon extension (LUX PCR) with special regard to the possibility of vertical transmission. This is the first time that presence of GHPV was confirmed in goslings that died within 4 days after hatching showing typical symptoms of HNEG, which indicates vertical transmissibility as the shortest incubation period of HNEG is 6 days. The way of viral transmission is a key issue and thus the disease control measurements and HNEG epizootiology may be revised based on the findings of this study.

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Detection of Goose and Muscovy Duck Parvoviruses using Polymerase Chain Reaction-Restriction Enzyme Fragment Length Polymorphism Analysis

Cited by 28 publications

References 10 publications

Isolation and characterization of a subtype C avian metapneumovirus circulating in Muscovy ducks in China

Isolation and characterization of a subtype C avian metapneumovirus circulating in Muscovy ducks in China

Pathology of goose haemorrhagic polyomavirus infection in goose embryos

Case report of goose haemorrhagic polyomavirus in 4-day-old goslings indicating vertical transmissibility

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