Detection of grape phylloxera (<i>Daktulosphaira vitifoliae</i>
 Fitch) by real-time quantitative PCR: development of a soil sampling protocol

Giblot‐Ducray, D.; Correll, Ray; Collins, Cassandra; Nankivell, Alan; Downs, Alex M.; Pearce, Inca; McKay, Alan; Ophel-Keller, K.

doi:10.1111/ajgw.12237

Cited by 7 publications

(6 citation statements)

References 11 publications

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“…Vineyard managers are already encouraged to collect EM38 data for other aspects of grapevine production, so there should be little increased cost . Several new methods for early phylloxera detection are currently being developed and trialled, including chemical and pigment fingerprinting of metabolites as biomarkers in leaves, a phylloxera‐specific DNA probe, emergence traps and, more recently, the use of unmanned aerial vehicles and sniffer dogs . The efficiency of all these new methods has the potential to be improved by spatially targeted surveillance designs like those considered in this study.…”

Section: Discussionmentioning

confidence: 99%

Accounting for spatially heterogeneous conditions in local‐scale surveillance strategies: case study of the biosecurity insect pest, grape phylloxera (Daktulosphaira vitifoliae (Fitch))

Triska

Powell

Collins

et al. 2018

Pest Management Science

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Targeting surveillance strategies based on local-scale spatial heterogeneity can decrease the time to detection without increasing the survey cost, and surveillance that targets highly suitable soil is the most efficient strategy for detecting new incursions. In addition, combining targeted surveillance strategies with buffer zones and hygiene procedures, and updating surveillance strategies as additional species information becomes available, will further decrease the risk of pest spread. © 2018 Society of Chemical Industry.

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Section: Discussionmentioning

confidence: 99%

Accounting for spatially heterogeneous conditions in local‐scale surveillance strategies: case study of the biosecurity insect pest, grape phylloxera (Daktulosphaira vitifoliae (Fitch))

Triska

Powell

Collins

et al. 2018

Pest Management Science

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“…Six commercial cultivars were represented among the 35 samples, all with unknown halo blight disease status. DNA was extracted from 200 g of seed from each sample using the SARDI proprietary protocol that has been developed for soil DNA extraction and has been successfully applied to a range of environmental samples, including grain (D. Giblot-Ducray unpublished data, Giblot-Ducray et al 2016;Haling et al 2011). Seeds were not washed prior to extraction.…”

Section: Sample Collectionmentioning

confidence: 99%

Evaluating molecular diagnostic techniques for seed detection of Pseudomonas savastanoi pv. phaseolicola, causal agent of halo blight disease in mungbean (Vigna radiata)

Noble

Williams

Douglas³

et al. 2022

Australasian Plant Pathol.

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Halo blight of mungbean (Vigna radiata var. radiata) is caused by the bacterium Pseudomonas savastanoi pv. phaseolicola. This pathogen is transmitted via infected seed, facilitating the spread of the disease into new cultivated areas. Prospective mungbean seed crops are currently subjected to visual inspection as a means of determining disease status, however, this is a poor method that relies on visible symptoms and does not account for latent infections. A range of molecular diagnostics targeting P. savastanoi pv. phaseolicola have been developed, but these have not been deployed on seeds. Quantitative PCR (qPCR) SYBR assay, hydrolysis probe, and conventional PCR, using the same primers were optimised against a plate-truthed dilution series of P. savastanoi pv. phaseolicola. The detection limit of the conventional PCR assay was approximately 9,000 CFU µl-1, while both qPCR assays could detect 9 CFU µl-1. These tests were then used to screen DNA extracted from 200 g allotments of 38 seed lots comprising six mungbean cultivars representing the primary Australian production area, and two seed lots of known infection status. Of these, the pathogen was detected in six seed lots by conventional PCR. The SYBR assay and hydrolysis probe methods detected 20 and 24 infected seed lots respectively. This shows that the hydrolysis probe method was the most effective at diagnosing the presence of P. savastanoi pv. phaseolicola in mungbean seed, providing a valuable molecular diagnostic to aid in integrated disease management and seed certification, substantially mitigating losses to halo blight disease.

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“…At this point, it is also worth mentioning the approaches implemented in grape phylloxera management strategies for the early detection of the insect in vineyards via DNA-based methods [44,45,46]. The development of a molecular method for grape phylloxera detection is especially relevant in grape-growing regions, where surveillance and prevention (through quarantine regulations) are the main factors for pest management [47].…”

Section: Relevance Of Studying Population Structure In Grape Phyllmentioning

confidence: 99%

Use of DNA Markers for Grape Phylloxera Population and Evolutionary Genetics: From RAPDs to SSRs and Beyond

Tello

Forneck

2019

Insects

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Grape phylloxera (Daktulosphaira vitifoliae Fitch) is a major pest of cultivated grapevines (Vitis spp.), occurring in virtually all viticultural regions around the world. Different grape phylloxera strains can be found at varying levels on leaves and roots on both own-rooted plants and in plants grafted onto partially resistant rootstocks. Considering its relevance for the adequate management of the pest in infested vineyards, the analysis of its genetic diversity has received considerable attention from the scientific community in the last decades. Here, we review 25 years of DNA-based molecular markers applied to the analysis of the genetic structure and the reproductive mode of grape phylloxera in its native range and in different introduced regions. The use given to RAPD, AFLP, mtDNA sequencing and microsatellite (SSR) genetic markers for the analysis of grape phylloxera diversity is discussed, and an overview of the main findings obtained after their application to different populations collected in diverse regions all around the world is shown. Lastly, we explore how recent advancements in molecular biology and in modern high throughput genotyping technologies may be applied to better understand grape phylloxera natural diversity at a molecular level.

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Detection of grape phylloxera (Daktulosphaira vitifoliae Fitch) by real-time quantitative PCR: development of a soil sampling protocol

Cited by 7 publications

References 11 publications

Accounting for spatially heterogeneous conditions in local‐scale surveillance strategies: case study of the biosecurity insect pest, grape phylloxera (Daktulosphaira vitifoliae (Fitch))

Accounting for spatially heterogeneous conditions in local‐scale surveillance strategies: case study of the biosecurity insect pest, grape phylloxera (Daktulosphaira vitifoliae (Fitch))

Evaluating molecular diagnostic techniques for seed detection of Pseudomonas savastanoi pv. phaseolicola, causal agent of halo blight disease in mungbean (Vigna radiata)

Use of DNA Markers for Grape Phylloxera Population and Evolutionary Genetics: From RAPDs to SSRs and Beyond

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