Detection of H. pyhi Infection by Biopsy Urease, Histology, and Culture

Abstract

Detection of Helicobacter pylori in specimens obtained by endoscopy requires the gastroenterologist to select suitable patients for endoscopy, and to take an adequate number of biopsy specimens; there must also be correct cleaning of the biopsy forceps.

Effects of cagA+ and cagA– strains of Helicobacter pylori on the human gastric mucus layer thickness

“…Helicobacter pylori culture was performed using the methods described by Goodwin 24 and Glupczunski 25 . The bacterial viable count (colonization density) was assessed by direct counting of the bacterial colonies on the culture plates that were inoculated with the serial dilutions prepared from the bacterial homogenates using the method described by Collins and Lyne 26 .…”

Combined Activity of Azithromycin and Lansoprazole Against Helicobacter pylori

“…For primary isolation of H. pylori , biopsies were streaked out on Pylori agar® (Biomérieux, Nürtingen, Germany) which contains 10% horse plasma and an antibiotic mixture. The plates were incubated in a microaerophilic atmosphere (5–7% O 2 , 8–10% CO 2 ; Anaerocult C® gas generating kit, Merck, Darmstadt, Germany) at 37°C for up to 5 days, and H. pylori identified as described previously [13]. For subcultures, Columbia agar supplemented with 7% defibrinated horse blood (Oxoid, Wesel, Germany) without antibiotics was used.…”

Is <i>Helicobacter pylori</i> a Pathogenic Agent of the Cervix Uteri?

“…Biopsy samples (100 l) were cultured on Columbia agar containing 7% laked horse blood (Invitrogen, Auckland, New Zealand) with Dent H. pylori antibiotic supplement (Oxoid) and incubated at 37 ° C for up to 10 days in anaerobic jars (no catalyst) with CampyGen packs (Oxoid) [9] . Colonies were identifi ed as H. pylori by Gram staining, oxidase, catalase, and urease tests as previously described [9] .…”

“…Colonies were identifi ed as H. pylori by Gram staining, oxidase, catalase, and urease tests as previously described [9] . NCTC 11637 strain of H. pylori was used as a positive control.…”