Detection of H-Y (Male) Antigen on Mouse Lymph Node Cells by the Cell to Cell Cytotoxicity Test

Antibody response to DBY minor histocompatibility antigen is induced after allogeneic stem cell transplantation and in healthy female donors

“…24,25 Subsequent studies demonstrated the presence of MHC-restricted T-cell responses to these antigens. [26][27][28] These results were extended to humans when H-Y-specific cell-mediated cytotoxicity was identified in a female patient with aplastic anemia who rejected a bone marrow graft from her HLA-identical brother. 29 In this patient, graft rejection was also associated with the development of IgM antibodies that were selectively cytolytic for male cells.…”

Immune Surveillance by Natural IgM Is Required for Early Neoantigen Recognition and Initiation of Adaptive Immunity

“…Although not a cancer model per se, this neoantigen-expressing cell model does not require PAMPs for its recognition and elimination, as new non-MHC-associated proteins are introduced into female mice. It is well established that adoptively transferred male cells are completely rejected (i.e., eliminated) in syngeneic female mice (53)(54)(55)(56)(57). This is due to the development of a CTL response against the Y chromosome-associated antigens, which are absent in female mice (27,50).…”

Role of the Newborn’s Sex in Mixed Maternal-Newborn Lymphocyte Culture Reactivity

“…Evidence for the presence and immunological involvement of H-Y minor transplantation antigens were obtained by sero logical and cell-mediated reactions in experiments on animal and human cells [18][19][20][21][22], In histocompatibility reactions the immunological nature of H-Y incompatible skin graft rejection was observed from the accelerated retrogression of second set male skin grafts in male sen sitized adult female mice [23], The immunological in volvement of the weak H-Y histocompatibility antigens in the fetomaternal relationship based on the mothers' ability to recognize male specific fetal antigens is indi cated by the following: (a) the ratio of male to female live births decreased with the increasing parity, whereas this ratio increased in stillbirths; (b) the number of preceding boys can influence the sex ratio of subsequent children and the intervals between births, and (c) fetal sex can influence maternal HLA antibody formation [9,20]. Recently a significantly increased male to female sex ratio was found in HLA-DR identical parents and the authors suggest that H-Y male minor histocompatibility antigens may provide themselves with the antigenic stimulus capable of eliciting the maternal immunological response [21,22], Although MLC reactivity usually measures cellular responses to class II antigens, in an investigation now in progress in our laboratory a significantly increased reactivity in MLC could be observed with maternal cells and male as compared to female offspring cells in families where male and female siblings were sharing DR identical antigens as well as in families where male and female siblings were not sharing these antigens.…”