Detection of HCV and GBV-C/HGV infection by multiplex PCR in plasma samples of transfused subjects

Caudai, Cinzia; Padula, M. G.; Bettini, Vanessa; Valensin, Pier Egisto

doi:10.1016/s0166-0934(97)00171-7

Cited by 11 publications

(6 citation statements)

References 17 publications

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“…69-88) and CC2 antisense (5′-GAGGTTTAGGATTCGTGCTC nt. 344-362) [6] and the "inner" primers were CC3 sense (5′-GAGTACACCGGAATTGCCAGG nt. 161-181) and CC4 antisense (5′-GCAAGCACCCTATCAG-GCAGT nt.…”

Section: Pcr Studiesmentioning

confidence: 99%

Hepatitis C virus infection and myositis: a polymerase chain reaction study

et al. 2000

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Muscle biopsy tissue from a patient with chronic hepatitis, who was hepatitis C virus (HCV) positive and showed slight weakness of the right arm and leg associated with increased serum creatine kinase levels, was studied using immunocytochemical and polymerase chain reaction (PCR) techniques. Muscle biopsy showed changes compatible with an inflammatory myopathy. Immunohistochemical studies included the use of monoclonal antibodies against human T lymphocytes, macrophages, immunoglobulins, major histocompatibility complex class I molecules (MHC-I), and the neoantigens of the terminal C5b-9 complement membrane attack complex (MAC). In addition to confirming the potential importance of cytotoxic T cells and MHC-I antigen expression in inducing muscle pathology, we demonstrated MAC deposition and the presence of HCV-RNA in the muscle of our patient, suggesting that direct involvement of the virus leading to complement activation might be important in inducing muscle damage.

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Section: Pcr Studiesmentioning

confidence: 99%

Hepatitis C virus infection and myositis: a polymerase chain reaction study

et al. 2000

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“…In so doing, the amount of information generated per assay can be increased, and cost of consumables and labor can be reduced (Henegariu et al 1997). Presently, PCR is the most commonly used technique especially for marker assisted selection (MAS) (Katula-Debreceni et al 2010), genetic purity testing (Sundaram et al 2008), genetic disease diagnosis (Caudai et al 1998), and detection of GMO crops (Zhang et al 2010;Onishi et al 2005;Demeke and Ratnayaka 2008). Considering these points, we evaluated the direct-PCR protocol for amplification of multiplexed SSRs in cotton, moth bean, and pearl millet, and the results were found consistent and reproducible compared to those data collected for control DNA (Fig.…”

Section: Multiplex-pcrmentioning

confidence: 99%

A Simple and Non-destructive Method of Direct-PCR for Plant Systems

et al. 2011

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To date, PCR is a fundamental tool for most of the research concerning plant diversity analysis, marker-assisted selection, genetic purity testing, disease diagnostics, and transgene analysis. In all of these analyses, good-quality DNA serves as a template for amplification of target sequences. Extraction of good-quality DNA requires many steps, making the whole process time consuming, tedious, labor intensive, and expensive due to costlier and toxic chemicals. To overcome these preparatory steps from PCRbased DNA amplification, we have developed a direct-PCR amplification method for plants without isolating DNA. The method is unique and beneficial over some previously described methods of direct-PCR which fail due to inefficient amplification of target DNA in the presence of PCR inhibitors and crop specificity. Moreover, such methods are non-specific and, being destructive, cannot be replicated; one cannot completely rely on them due to lack of reproducibility. This method was streamlined from our earlier observation that alcohol-desiccated tissues maintain intact DNA for a long time. This method is specific, rapid, and, being nondestructive, allows replication, giving advantages over existing methods. The method was tested over a wide range of plant species and found very effective and quick in generating data. The method was successfully used to test the genetic purity of pearl millet hybrid (RHB-127) and its restorer (RIB 3135-18) and CMS line (ICMA 93333A). Our method is especially important for developing inexpensive and high-throughput non-invasive genetic analyses.

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“…HCV and GB virus type C (GBV-C)/HGV genomes in plasma samples from transfused subjects have also been amplified by a multiplex PCR (16). The test was evaluated retrospectively in 50 plasma samples in comparison with the results of serology.…”

Section: Other Applicationsmentioning

confidence: 99%