Detection of Helicobacter pylori in stool specimens by non-invasive antigen enzyme immunoassay in children: multicentre Italian study

Oderda, Giuseppina; Rapa, Anna; Ronchi, Barbara; Lerro, Piero; Pastore, M.; Staiano, Annamaria; Angelis, G.L. De; Strisciuglio, Pietro

doi:10.1136/bmj.320.7231.347

Cited by 137 publications

(114 citation statements)

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“…Although the detection of H. pylori antigen in stool has a sensitivity and specificity of 90−95%, other tests (such as urease test and culture of the organism from gastric biopsies) or combination tests may have improved our detection rate (6,9). Nevertheless, this study is the first in Khuzestan province, south-west of Iran and provide insights into the epidemiology of H. pylori in this area.…”

Section: Discussionmentioning

confidence: 88%

“…pylori infection is thought to play an etiologic role in several gastro-duodenal diseases. In epidemiological studies, H. pylori antigens (HpSA) in stool could offer high sensitivity and specificity (9). HpSA is a noninvasive method for determining the presence of H. pylori organisms.…”

Section: Discussionmentioning

confidence: 99%

“…Many studies support the diagnostic value of H. pylori antigens in stool samples by enzyme immunoassay (EIA) (8).This test was approved in 1998 for both diagnosis and monitoring the response to treatment of H. pylori infection in adult patients (9). Recently, a study performed by Falsafi T et al using stool test for detection of H. pylori antigen in children and adolescents from Tehran demonstrated a high prevalence of infection (70%) in cases with a family history of H. pylori infection or peptic ulcer disease (10).…”

Section: Introductionmentioning

confidence: 99%

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Prevalence of Helicobacter pylori Infection evaluated by Stool antigen test in Khuzestan Province since September to October 2009, south-west of Iran: a population based study

Talaiezadeh

Borhani

Moosavian

et al. 2013

Jundishapur J Microbiol

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Background: Although infection with Helicobacter pylori is a global health problem, its prevalence is different among countries. Serologic tests for the diagnosis of H. pylori infection are limited by low accuracy rates and lack of validation. Recent studies indicate that the stool antigen test has an acceptable level of accuracy. Objectives: Our objective was to investigate the prevalence of H. pylori infection in Khuzestan province, south-west of Iran by stool antigen test and to evaluate possible risk factors for the H. pylori infection. Patients and Methods: In this study, from September to October 2009, 861 healthy individuals aged 0-80 years were sampled by cluster sampling from 4.5 million inhabitants of the province. Infection with H. pylori was evaluated by detection of H. pylori antigens in stool (HpSA). Epidemiologic data of each subject were determined by filling up a questionnaire. Results: The overall HpSA positivity was (53.5%, CI 95% = 50.2-56.84%) and the mean age of infected cases was greater than that of non-infected ones (29.2 yrs vs. 24.5 yrs) (P < 0.001). HpSA positivity increased with age. The peak prevalence was reached in the 41-50 years age group (66%). No association was detected between H. pylori positivity and gender. Additionally, there was no significant relationship between H. pylori infection and previous hospital admission, gastrointestinal symptoms, and living area (P > 0.05). Conclusions: H. pylori infection is highly prevalent (57%) in Khuzestan province, south-west of Iran. Low educational levels, low socioeconomic jobs, and increasing age were related to high prevalence of H. pylori infection in this area.

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Section: Discussionmentioning

confidence: 88%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Prevalence of Helicobacter pylori Infection evaluated by Stool antigen test in Khuzestan Province since September to October 2009, south-west of Iran: a population based study

Talaiezadeh

Borhani

Moosavian

et al. 2013

Jundishapur J Microbiol

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“…The stool antigen test has been presented as a cheaper examination which is easier to perform than UBT, and has also been shown to be successful in children. 19,20 Its diagnostic yield is higher than 90%, on average, in many studies performed in the pre-treatment setting, 21,22 and this renders HpSA a valid alternative to UBT as a non-invasive method for the diagnosis of H. pylori infection in epidemiological investigations or in those clinical situations in which endoscopy can be avoided.…”

Section: Discussionmentioning

confidence: 99%

Stool antigen assay (HpSA) is less reliable than urea breath test for post‐treatment diagnosis of Helicobacter pylori infection

Bilardi¹,

Biagini²,

Dulbecco³

et al. 2002

Aliment Pharmacol Ther

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“…In addition, the accuracy of the test has been confirmed in children. Moreover, this test may be the optimum test to screen and confirm the success of eradication therapy even as early as 2 weeks after the end of therapy (Ni et al 2000;Oderda et al 2000).…”

Section: Patientsmentioning

confidence: 99%

Quantitative Correlation of Helicobacter pylori Stool Antigen (HpSA) Test with the Severity of H. pylori-Related Gastritis

Adiloğlu

Işler

Goren

et al. 2007

Tohoku J. Exp. Med.

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The Helicobacter pylori (H. pylori) load in both stomach and stool and the resulting severity of gastritis are important criteria in validating the status of H. pylori infection. We aimed to assess the reliability of the H. pylori stool antigen (HpSA) test for the primary diagnosis of H. pylori infection by calculating the best cut-off value to obtain the highest sensitivity and specificity in dyspeptic patients. We also investigated the correlation of HpSA test with the severity of gastritis and H. pylori load. The H. pylori statuses of 95 patients were evaluated by the positivity of both rapid urease test and microscopic detection of H. pylori in biopsy specimens, 88 subjects of whom were H. pylori positive. The sensitivity and specificity of the HpSA test were 51.1% (45/88) and 100% (7/7), respectively, according to the manufacturer's recommended cut-off value of 0.16. However, with the best cut-off value of 0.048, calculated by receiver operator characteristics analysis, the sensitivity of the test increased to 92.0% (81/88) with the same specificity. High values of the HpSA test were correlated with high scores of corpus H. pylori load and the severity of antrum and corpus inflammation ( p < 0.05). With the best cut-off value of the HpSA test, the primary diagnosis of H. pylori infection can be made with higher sensitivity and specificity. The HpSA test is a helpful tool that evaluates the severity of H. pylori infection and the degree of gastric inflammatory activity and gastric H. pylori load.

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Detection of Helicobacter pylori in stool specimens by non-invasive antigen enzyme immunoassay in children: multicentre Italian study

Cited by 137 publications

References 2 publications

Prevalence of Helicobacter pylori Infection evaluated by Stool antigen test in Khuzestan Province since September to October 2009, south-west of Iran: a population based study

Prevalence of Helicobacter pylori Infection evaluated by Stool antigen test in Khuzestan Province since September to October 2009, south-west of Iran: a population based study

Stool antigen assay (HpSA) is less reliable than urea breath test for post‐treatment diagnosis of Helicobacter pylori infection

Quantitative Correlation of Helicobacter pylori Stool Antigen (HpSA) Test with the Severity of H. pylori-Related Gastritis

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