Detection of Helicobacter pylori in gastric biopsy and resection specimens

Babić, Tatjana; Basic, Hakija; Selimović-Miljković, Biljana; Kocić, Branislava; Tasić, Gordana

doi:10.2298/vsp0501039b

Cited by 3 publications

(1 citation statement)

References 10 publications

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“…In relevance with this, in the present study, the low socio economic group (93%) was observed to be more susceptible to Helicobacter pylori infection. Javed et al [14] also states that the rate of Helicobacter pylori infection is directly propotional to age with a maximum number seen in the age group of 46-55yrs. In the age group of 41-50 years, the maximum occurrence of Helicobacter pylori associated gastritis was noted (Adisa et al [15]).…”

Section: Discussionmentioning

confidence: 99%

Role of Immunohistochemistry Versus he Stain in Helicobacter pylori Detection in Gastric Lesions

Narain

Karkuzhali

Kumar

et al. 2021

JPRI

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Helicobacter pylori are a spiral campylobacter like bacteria which infects the stomach causing chronic active gastritis. This can result in peptic ulcer disease, gastric adenocarcinoma, and mucosa-associated lymphoid tissue lymphoma. In 1989, studies involving 16S ribosomal RNA gene sequencing and others revealed that the bacterium does not come under the genus Campylobacter. Hence the bacterium was classified under a unique genus named Helicobacter. This term is taken from the Greek language with helix meaning - "spiral" or "coil" and pylori meaning- gatekeeper (pylorus of stomach). This bacterium is said to penetrate the pylori or mucoid lining of the stomach. When infected during the early stages of life, people develop intense inflammation that may be followed by atrophic gastritis which serves as a risk factor for gastric ulcer, gastric cancer or both. Being infected during later stages of life changes the gastric system leading to duodenal ulcer. The present study analyzes the role of Immunohistochemistry versus Hematoxylin and Eosin and special stains in detecting Helicobacter pylori in gastric lesions.

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Section: Discussionmentioning

confidence: 99%

Role of Immunohistochemistry Versus he Stain in Helicobacter pylori Detection in Gastric Lesions

Narain

Karkuzhali

Kumar

et al. 2021

JPRI

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Histopathological Changes in Helicobacter pylori Associated Gastritis and Scope of Special Stain and Immunohistochemistry as Diagnostic Aids

Elias¹,

Gisuthan²,

A.S³

2020

jebmh

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BACKGROUND Helicobacter pylori associated chronic gastritis plays a vital role in the development of majority of gastric adenocarcinomas and most gastric MALT (Mucosa Associated Lymphoid Tissue) lymphomas. Many diagnostic methods are available for the identification of this organism. However, in gastroenterology practice, histopathological examination of biopsy samples provides visual identification of the pathogen and the associated mucosal changes with special stains like Giemsa. The aim of this study was to evaluate the efficacy of three stains H & E- (Haematoxylin and Eosin), Giemsa and IHC (Immunohistochemistry) in the identification of H. pylori. Associated histologic changes were noted and the relationship between the degree of colonisation and the activity and chronicity of gastritis were analysed. METHODS 585 gastric biopsies taken from dyspeptic patients were evaluated for gastritis, based on updated Sydney System. In 250 randomly selected cases, three staining methods were used. RESULTS Out of 585 cases, 413 (70.60 %) had features of chronic gastritis. Mild chronic gastritis was the commonest finding and is seen in most cases of mild H. pylori colonisation. When activity was monitored, mild activity was the most frequent finding [225 (38.46 %)]. Majority of the severe activity cases showed severe H. pylori colonisation. 13.16 %, 4.79 % and 7.35 % showed intestinal metaplasia, atrophy and dysplastic changes respectively. Out of 250 cases, H & E and Giemsa stains showed 45.6 % and 57.2 % positivity while IHC demonstrated maximum number of positivity (156 cases - 62.4 %). Sensitivity and specificity of H & E was found to be 77.90 % and 98.95 %, positive predictive value was 99.13 % and negative predictive value was 69.18 %. For Giemsa stain, sensitivity was 91.67 %, specificity was 100 %, positive predictive value was 100 % and negative predictive value was 87.85 %. DISCUSSION H. pylori gastritis was a frequent finding in dyspeptic patients in southern part of India. When chi-square test was done, a significant statistical relationship between the severity of H. pylori colonisation, activity and chronicity of gastritis was noted. P value was < 0.001. With the use of special stain, Giemsa and ancillary techniques like IHC, the detection rate of H. pylori was enhanced considerably. CONCLUSIONS With increasing number of H. pylori in the mucosa, there was increase in the chronicity and activity of gastritis. Although immunohistochemistry revealed more cases of H. pylori, Giemsa can be a cost-effective substitute, because of its high specificity and positive predictive value. KEYWORDS H. pylori Gastritis, Giemsa, Haematoxylin and Eosin Stain, Immunohistochemistry

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A comparative study between immunohistochemistry, hematoxylin & eosin and geimsa stain for helicobacter pylori detection in chronic gastritis

Dharshini.M¹,

Du.V²

2017

Int J Pharm Bio Sci

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Detection of Helicobacter pylori in gastric biopsy and resection specimens

Abstract: Immunohistochemical identification of H. pylori was better than Giemsa stain for detecting that organism.

Cited by 3 publications

References 10 publications

Role of Immunohistochemistry Versus he Stain in Helicobacter pylori Detection in Gastric Lesions

Role of Immunohistochemistry Versus he Stain in Helicobacter pylori Detection in Gastric Lesions

Histopathological Changes in Helicobacter pylori Associated Gastritis and Scope of Special Stain and Immunohistochemistry as Diagnostic Aids

A comparative study between immunohistochemistry, hematoxylin & eosin and geimsa stain for helicobacter pylori detection in chronic gastritis

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