Detection of Helicobacterpylori Antigens among Patients with Gastroenteritis in Erbil City, Iraq

Abstract

Helicobacter pylori is a significant pathogen of the gastrointestinal tract infection connected with gastritis, peptic ulcers, and gastric carcinoma. Its infection influence more than half of the world’s inhabitants. This study aims to reveal the prevalence rate of H. pylori infection among patients with gastroenteritis and to investigate the risk factors such as age, gender, residency, blood group and rhesus factors related to this infection in Erbil city. Of 300 stool specimens from patients presenting with gastroenteritis who were admitted to Rizgary Teaching Hospital, Erbil, Iraq, from September 2018 to February 2019 were collected and screened for the presence of H. pylori antigens using rapid immunochromatographic assay (Camp Medica Group, Bucharest, Romania). A questionnaire sheet was prepared and used for each study subject. Of 300 samples examined, H. pylori were detected in 79 samples (26.33%). The highest rate of H. pylori infection was founded among the age group 21–30 years, but there were no significant differences between them (P > 0.05). A significant relationship between H. pylori infection and gender (male 19.05% and female 33.33%) was recorded (P < 0.05). There was a significant association between H. pylori infection and ABO blood group among patients (P < 0.05), but there is no significant association between H. pylori infection and the type of rhesus factor (P > 0.05) that H. pylori infection was higher in rhesus factor negative compared to rhesus factor positive. The prevalence was significantly higher among rural area (55.56%) than urban area (23.44%) (P < 0.05). We concluded that the spread of H. pylori positive rate was high among patients with gastroenteritis in Erbil city. The great prevalence of H. pylori was founded in the patients with O blood group, urban area, and females. There was no significant association between H. pylori infection and age groups and rhesus factor.

Serological Diagnosis and Epidemiological impact of Helicobacter pylori infection on human health in Diyala Governorate, Iraq

“…Laboratory detection of H. pylori antibodies in blood samples was carried out using H. pylori antibody test card H. pylori Ab combo rapid test (device kit). The test was performed according to the previously published protocol [16].…”

Identifying and Isolating of H. pylori in patients who are experiencing Inflammation in their Gastrointestinal tract

“…The invasive technique uses polymerase chain reaction, histological testing, culture, endoscopy, rapid urease test and biopsy. The stool antigen test, serological testing, urinary antibody test and the urea breath test are examples of non-invasive techniques (Majeed and Abdullah, 2019). Immunochromatographic testing is one of the serological methods that is frequently employed to diagnose H. pylori due to low cost and easy availability at any laboratories (Rishma et al, 2018).…”

“…In context of age-specific frequency of H. pylori, the age group(31)(32)(33)(34)(35)(36)(37)(38)(39)(40) had greatest percentage with (14.7%), followed by the age group (41-50) with (13.2%), and then the age group(21)(22)(23)(24)(25)(26)(27)(28)(29)(30) with (11.7%) while lowest percentages in age groups < 10 and ˃ 60 years old. This is consistent with findings published by Udoh and Obaseki (2012), who discovered that individuals with H. pylori have a greater rate of infectivity in the adult or younger age group.…”