Detection of hepatitis a virus in wild shellfish

Crance, J.M.; Apaire-Marchais, Véronique; Lévêque, F.; Beril, C.; Guyader, F. Le; Jouan, A.; Schwartzbrod, L.; Billaudel, S.

doi:10.1016/0025-326x(94)00193-d

Cited by 4 publications

(1 citation statement)

References 33 publications

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“…As an outbreak (about 660 cases presumably linked to shellfish consumption) occurred during the winter of 1992 (37), this contamination may reflect residual virus. In a study of the same area, infectious particles of HAV were found 3 months after the outbreak (9). This contamination may also be a consequence of the presence of virus in sewage despite the decline of seropositivity for HAV in France (27).…”

Section: Discussionmentioning

confidence: 99%

Detection of hepatitis A virus, rotavirus, and enterovirus in naturally contaminated shellfish and sediment by reverse transcription-seminested PCR

Guyader

Dubois

Ménard

et al. 1994

Appl Environ Microbiol

176

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A reverse transcription-PCR method was developed to detect enterovirus (EV), hepatitis A virus (HAV), and rotavirus (RV) RNAs in shellfish and sediment. The method was first tested under experimental conditions by using virus-spiked shellfish to evaluate assay sensitivity. The use of CC41 cellulose was found to be efficient for removing inhibitors of RV detection. For sediment samples, a Sephadex column was used to allow the detection of EV and HAV RNAs. The specificity of amplified products was controlled by hybridization with digoxigeninlabeled oligoprobes. The method was then applied to naturally contaminated shellfish and sediments. EV,

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Section: Discussionmentioning

confidence: 99%

Detection of hepatitis A virus, rotavirus, and enterovirus in naturally contaminated shellfish and sediment by reverse transcription-seminested PCR

Guyader

Dubois

Ménard

et al. 1994

Appl Environ Microbiol

176

View full text Add to dashboard Cite

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Eutrophication, Water Borne Pathogens and Xenobiotic Compounds: Environmental Risks and Challenges

1999

Marine Pollution Bulletin

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Direct sequencing of hepatitis A virus strains isolated during an epidemic in France

Apaire-Marchais

Robertson

Aubineau-Ferre

et al. 1995

Appl Environ Microbiol

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Direct sequencing of PCR products was used to study the VP1 region of the hepatitis A virus (HAV) genome (position 2199 to 2356) of nine strains isolated from human stools collected during a hepatitis A epidemic (western France, 1992), three strains from environmental samples (1990, 1991, and 1992), and two HAV cell culture isolates (the French strain CF53/Lyon and strain CLF). These viruses differed from CF53/Lyon (genotype I) by between 1 and 10.3%, and results indicated the existence of two groups of strains belonging to two different subgenotypes (IA and IB). With this sequencing technique it was possible to monitor the epidemiology of HAV and study its relations.

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Detection of hepatitis a virus in wild shellfish

Cited by 4 publications

References 33 publications

Detection of hepatitis A virus, rotavirus, and enterovirus in naturally contaminated shellfish and sediment by reverse transcription-seminested PCR

Detection of hepatitis A virus, rotavirus, and enterovirus in naturally contaminated shellfish and sediment by reverse transcription-seminested PCR

Eutrophication, Water Borne Pathogens and Xenobiotic Compounds: Environmental Risks and Challenges

Direct sequencing of hepatitis A virus strains isolated during an epidemic in France

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